Comparative expression analysis of let-7 microRNAs during ovary development in Megalobrama amblycephala

Liang, Tian; Chen, Yulong; Gul, Yasmeen; Zhao, Bowen; Gao, Zexia

doi:10.1007/s10695-019-00624-7

Cited by 18 publications

(11 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Metastases are the main reason for the high mortality in these patients [ 70 ]. Studies in model organisms have revealed a gradual increase in ovarian and pituitary let-7 expression, including let-7d, during ovarian development, suggesting a potential role of this microRNA in ovarian function [ 71 ].…”

Section: Let-7d and Ovarian Cancermentioning

confidence: 99%

The Role of microRNA Let-7d in Female Malignancies and Diseases of the Female Reproductive Tract

Santis¹,

Götte²

2021

IJMS

View full text Add to dashboard Cite

microRNAs are small noncoding RNAs that regulate gene expression at the posttranscriptional level. Let-7d is a microRNA of the conserved let-7 family that is dysregulated in female malignancies including breast cancer, ovarian cancer, endometrial cancer, and cervical cancer. Moreover, a dysregulation is observed in endometriosis and pregnancy-associated diseases such as preeclampsia and fetal growth restriction. Let-7d expression is regulated by cytokines and steroids, involving transcriptional regulation by OCT4, MYC and p53, as well as posttranscriptional regulation via LIN28 and ADAR. By downregulating a wide range of relevant mRNA targets, let-7d affects cellular processes that drive disease progression such as cell proliferation, apoptosis (resistance), angiogenesis and immune cell function. In an oncological context, let-7d has a tumor-suppressive function, although some of its functions are context-dependent. Notably, its expression is associated with improved therapeutic responses to chemotherapy in breast and ovarian cancer. Studies in mouse models have furthermore revealed important roles in uterine development and function, with implications for obstetric diseases. Apart from a possible utility as a diagnostic blood-based biomarker, pharmacological modulation of let-7d emerges as a promising therapeutic concept in a variety of female disease conditions.

show abstract

Section: Let-7d and Ovarian Cancermentioning

confidence: 99%

The Role of microRNA Let-7d in Female Malignancies and Diseases of the Female Reproductive Tract

Santis¹,

Götte²

2021

IJMS

View full text Add to dashboard Cite

show abstract

“…The Let-7 family of miRNAs plays significant roles in regulating gene expression, participating in a large variety of physiological activities [9]. Previous reports have indicated that miRNAs including let-7 possess the ability to suppress certain diseases such as HNSCC [10].…”

Section: Introductionmentioning

confidence: 99%

The let-7 family of microRNAs suppresses immune evasion in head and neck squamous cell carcinoma by promoting PD-L1 degradation

Liu

Han

et al. 2019

Cell Commun Signal

View full text Add to dashboard Cite

Background: Accumulation of immunosuppressive protein programmed death-ligand 1 (PD-L1) has been documented in several cancers and contributes to the evasion of the host immune system. However, cancer cellintrinsic signaling-dependent control of PD-L1 expression remains to be elucidated. Herein, we aimed to identify the let-7 family of microRNAs as candidates that up-regulate tumor cell PD-L1 expression and mediates immune evasion of head and neck squamous cell carcinoma (HNSCC). Methods: The expression of let-7 family and PD-L1 was quantified in HNSCC tissues and adjacent normal tissues. PD-L1 degradation was evaluated in HNSCC cells in response to elevated expressions of let-7a or let-7b. The regulation of let-7 family on PD-L1 degradation through a mechanism involving T-cell factor-4 (TCF-4) control of βcatenin/STT3 pathway was evaluated. Immune recognition of HNSCC in vivo was examined in subcutaneous tumorbearing C3H mice in the presence of let-7a/b and/or CTLA-4 antibody. Results: The let-7 family were significantly down-regulated in the context of HNSCC, sharing a negative correlation with PD-L1 expression. Glycosylated PD-L1 was detected in HNSCC cells, which was reduced by let-7a/b overexpression. TCF-4, the target of let-7a/b, activated the β-catenin/STT3 pathway and promoted PD-L1 degradation. In vivo analysis demonstrated that let-7a/b over-expression potentiated anticancer immunotherapy by CTLA-4 blockade. Conclusions: Taken together, our findings highlight targeting let-7 family as a potential strategy to enhance immune checkpoint therapy for HNSCC.

show abstract

“…Similarly, the miR‐184 and miR‐100 were also predominantly expressed in testis of the Chinese mitten crab E. sinensis like the swimming crab P. trituberculatus and the mud crab S. paramamosain (Figure 3; Jia et al, 2018; Meng et al, 2018), suggesting that miR‐184 and miR‐100 could have conserved roles in testicular development. Additionally, several let‐7 family members exhibited high expression in gonads and act important roles during gonadal development in fishes, birds, and mammals (Bizuayehu & Babiak, 2014; Fagegaltier et al, 2014; Gu et al, 2014; Huang et al, 2011; Juanchich et al, 2013; Kang et al, 2013; Lan et al, 2019; McBride et al, 2012). In the present study, high‐level expression of several let‐7 family members was also found in E. sinensis like the swimming crab (Meng et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Identification and characterization of sex‐biased and differentially expressed miRNAs in gonadal developments of the Chinese mitten crab, Eriocheir sinensis

Luo

Xiong

et al. 2021

Molecular Reproduction Devel

View full text Add to dashboard Cite

MicroRNA (miRNA) is a posttranscriptional downregulator that plays a vital role in a wide variety of biological processes. In this study, we constructed five ovarian and testicular small RNA libraries using two somatic libraries as reference controls for the identification of sex‐biased miRNAs and gonadal differentially expressed miRNAs (DEMs) of the Chinese mitten crab, Eriocheir sinensis. A total of 535 known and 243 novel miRNAs were identified, including 312 sex‐biased miRNAs and 402 gonadal DEMs. KEGG pathway analysis showed that DEM target genes were statistically enriched in MAPK, Wnt, and GnRH signaling pathway, and so on. A number of the sex‐biased miRNAs target genes associated with sex determination/differentiation, such as IAG, Dsx, Dmrt1, and Fem1, while others target the genes related to gonadal development, such as P450s, Wnt, Ef1, and Tra‐2c. Dual‐luciferase reporter assay in vitro further confirmed that miR‐34 and let‐7b can downregulate IAG expression, miR‐9‐5p, let‐7d, let‐7b, and miR‐8915 can downregulate Dsx. Taken together, these data strongly suggest a potential role for the sex‐biased miRNAs in sex determination/differentiation and gonadal development in the crab.

show abstract

Comparative expression analysis of let-7 microRNAs during ovary development in Megalobrama amblycephala

Cited by 18 publications

References 36 publications

The Role of microRNA Let-7d in Female Malignancies and Diseases of the Female Reproductive Tract

The Role of microRNA Let-7d in Female Malignancies and Diseases of the Female Reproductive Tract

The let-7 family of microRNAs suppresses immune evasion in head and neck squamous cell carcinoma by promoting PD-L1 degradation

Identification and characterization of sex‐biased and differentially expressed miRNAs in gonadal developments of the Chinese mitten crab, Eriocheir sinensis

Contact Info

Product

Resources

About