Comparative expression analysis of five WRKY genes from Tibetan hulless barley under various abiotic stresses between drought-resistant and sensitive genotype

Li, Huie; Guo, Qiqiang; Lan, Xiaozhong; Zhou, Qi; Wei, Na

doi:10.1007/s11738-013-1475-7

Cited by 14 publications

(8 citation statements)

References 33 publications

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“…WRKYs play important roles by controlling the expression of genes involved in various biological processes and biotic and abiotic stress responses [34,9,35]. There are limited HvWRKY6, HvWRKY40 and HvWRKY70 have exerted positive effects on wheat resistance to Puccinia triticina [39].…”

Section: Discussionmentioning

confidence: 99%

Fusarium culmorum ile Enfekte Edilen Arpa Çeşitlerinde WRKY Transkripsiyon Faktörlerinin Analizi

Uluhan

Keleş

Tufan

2019

International Journal of Life Sciences and Biotechnology

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One of the most critical problems of cereal breeding is Fusarium crown rot disease caused by various Fusarium species. Fusarium culmorum is one of the predominant pathogen in Turkey and causes serious product losses. In this study, the early response of barley cultivars upon F. culmorum infection were analyzed by disease severity and gene expression patterns of WRKY transcription factors. In that context, firstly, disease severities of 9 barley cultivars (Hordeum vulgare L. cvs. Epona, Escadre, Gazda, Oliver, Avcı 2002, Burakbey, Tarm 92, Manava, and Ramata) infected with F. culmorum were determined with disease index percentages. After 7 days of infection, Epona was more sensitive than the other cultivars while the lowest disease index was observed in Gazda. Expression analysis of HvWRKY6,

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Section: Discussionmentioning

confidence: 99%

Fusarium culmorum ile Enfekte Edilen Arpa Çeşitlerinde WRKY Transkripsiyon Faktörlerinin Analizi

Uluhan

Keleş

Tufan

2019

International Journal of Life Sciences and Biotechnology

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“…Peng et al ( 2014 ) showed that WRKY members' unigenes were mostly up-regulated under salt stress in cotton ( Gossypium hirsutum L.). They noted that some WRKY genes were expressed in the salt-tolerant genotype Earlistaple 7, but were repressed, weakly induced, or not induced at all in salt-sensitive Nan Dan Ba Di Da Hua, within 24 h. HvvWRKY2 was induced by salt stress in TR1 (Tolerant variety) but not in TS1 (Sensitive variety) (Li et al, 2014 ). Similarly, expression of wheat TaWRKY2 and TaWRKY19 was induced by salt, and both TaWRKY2 and TaWRKY19 enhanced salt tolerance in transgenic Arabidopsis plants compared with wild type (Niu et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of WRKY Genes Potentially Involved in Salt Stress Responses in Triticum turgidum L. ssp. durum

et al. 2017

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WRKY transcription factors are involved in multiple aspects of plant growth, development and responses to biotic stresses. Although they have been found to play roles in regulating plant responses to environmental stresses, these roles still need to be explored, especially those pertaining to crops. Durum wheat is the second most widely produced cereal in the world. Complex, large and unsequenced genomes, in addition to a lack of genomic resources, hinder the molecular characterization of tolerance mechanisms. This paper describes the isolation and characterization of five TdWRKY genes from durum wheat (Triticum turgidum L. ssp. durum). A PCR-based screening of a T. turgidum BAC genomic library using primers within the conserved region of WRKY genes resulted in the isolation of five BAC clones. Following sequencing fully the five BACs, fine annotation through Triannot pipeline revealed 74.6% of the entire sequences as transposable elements and a 3.2% gene content with genes organized as islands within oceans of TEs. Each BAC clone harbored a TdWRKY gene. The study showed a very extensive conservation of genomic structure between TdWRKYs and their orthologs from Brachypodium, barley, and T. aestivum. The structural features of TdWRKY proteins suggested that they are novel members of the WRKY family in durum wheat. TdWRKY1/2/4, TdWRKY3, and TdWRKY5 belong to the group Ia, IIa, and IIc, respectively. Enrichment of cis-regulatory elements related to stress responses in the promoters of some TdWRKY genes indicated their potential roles in mediating plant responses to a wide variety of environmental stresses. TdWRKY genes displayed different expression patterns in response to salt stress that distinguishes two durum wheat genotypes with contrasting salt stress tolerance phenotypes. TdWRKY genes tended to react earlier with a down-regulation in sensitive genotype leaves and with an up-regulation in tolerant genotype leaves. The TdWRKY transcripts levels in roots increased in tolerant genotype compared to sensitive genotype. The present results indicate that these genes might play some functional role in the salt tolerance in durum wheat.

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“…2). A previous report indicated that HvWRKY_IIc19 (HvvWRKY19) and HvWRKY_IIa4 (HvvWRKY2) are upregulated in vegetative organs of the resistant genotype but not in the sensitive genotype of barley under drought (Li et al 2014). Also, HvWRKY_IIa3 (HvWRKY1/38) is involved in response to abiotic stress including cold and drought stress at the seedling stage in barley via an ABA-independent way (Mar et al 2004).…”

Section: Resultsmentioning

confidence: 99%

Revision of the barley WRKY gene family phylogeny and expression analysis of the candidate genes in response to drought

Yazdani¹,

Sanjari²,

Zakaria³

et al. 2020

Biologia plant.

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The WRKY belongs to an important plant specific transcription factor families which are involved in response to various environmental stresses, as well as in growth and developmental processes. In the present report, a genome-wide identification and characterization of WRKY gene family in barley led to revision of HvWRKYs to 93 members. The phylogenetic tree was also reconstructed based on the full-length WRKY protein sequences in barley and Arabidopsis. HvWRKYs were classified into three major groups (I, II, and III) and group II was further divided to 5 subgroups (a to e). HvWRKYs were named after this classification. Interestingly, some specific motifs were discovered in subgroups IIa, IIb, and III. Analyzing the available microarray data revealed eight candidate WRKY genes which were up-regulated under drought and salinity stresses compared to the optimum conditions at seedling stage in barley. Expression profiles of these WRKY genes were validated by quantitative real-time PCR. All the investigated candidate genes (HvWRKY_IIc2, HvWRKY_III11, HvWRKY_ IIb2, HvWRKY_IId4, HvWRKY_III23, HvWRKY_IIa5 and HvWRKY_IIc19) except for HvWRKY_I8 were significantly upregulated by drought stress at the seedling stage in drought-tolerant genotype, indicating their role in drought tolerance. We hope the presented information would be helpful toward achieving drought tolerant cultivars through genetic engineering or molecular breeding.

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Comparative expression analysis of five WRKY genes from Tibetan hulless barley under various abiotic stresses between drought-resistant and sensitive genotype

Cited by 14 publications

References 33 publications

Fusarium culmorum ile Enfekte Edilen Arpa Çeşitlerinde WRKY Transkripsiyon Faktörlerinin Analizi

Fusarium culmorum ile Enfekte Edilen Arpa Çeşitlerinde WRKY Transkripsiyon Faktörlerinin Analizi

Comparative Analysis of WRKY Genes Potentially Involved in Salt Stress Responses in Triticum turgidum L. ssp. durum

Revision of the barley WRKY gene family phylogeny and expression analysis of the candidate genes in response to drought

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