"Comparative Experimental Studies of Few L-Type and T-Type Ca+2 Channel Blockers Against In-Ovo and In-Vitro Models of Angiogenesis"

Abstract

Angiogenesis is the development of new blood vessels from pre-existing one. The ion-channels on endothelium plays vital role in cell proliferation and related angiogenesis. We aimed to investigate the effects of L-type (Verapamil and Diltiazem) and T-type (Ethosuximide) Calcium Channel Blockers (CCBs) on neovascularization. The effects on neovascularization were investigated by in-ovo (CAM, Chick Chorioallantoic membrane) and in-vitro (aortic ring assay) methods. Each test drug was tested for at least 3 doses and the anti-angiogenic effect was compared with Suramin as standard and normal control groups. Various vital parameters were recorded during the experiment like the number of blood capillaries, sprouts formation, angiogenic score etc. The L-type Ca 2+ channel blockers Verapamil at the dose of 50µM, 110 and 220 µM/disk has shown significant (p < 0.001) reduction in the number of branching points in CAM assay. For the further confirmation, angiogenic activity was evaluated in vitro by rat aortic ring assay method; the area of sprouts was reduced by the medium and high dose of verapamil. Diltiazem has demonstrated modest anti-angiogenic activity by both the models, whereas T-type calcium channel blocker ethosuximide has not shown any effect on the neovascularization. Among all the tested drugs verapamil has shown the promising anti-angiogenic property. Thus verapamil and diltiazem may have antiangiogenic activity defines novel beneficial effects in angiogenic mediated pathological conditions in addition to their main indications in cardiovascular complications.blood capillaries supplement cancer growth by supplying nutrients, oxygen and by taking away waste products. Angiogenesis also supports Metastasis 5 . Angiogenesis is sequential and highly co-ordinated system involves several steps including basement membrane proteolysis, migration of individual endothelial cells, and endothelial cell proliferation 6 . Inhibition of angiogenesis and arresting development of blood capillaries has emerged as a promising strategy for the treatment of cancer 2 . Many researchers are

Comparative Experimental Studies of Angiotensin-Converting Enzyme Inhibitors and Angiotensin II Receptor Blockers against Two Different Models of Angiogenesis

“…In the present study, the CAM model of angiogenesis was carried out as per the procedure described in the earlier literature with some modification. 13 Briefly, fertilized eggs of white leghorn hen collected at day '0' from the local hatchery and incubated at 37°C for the next 48 hrs by keeping them vertically. After washing all the eggs with the distilled water, eggs were again incubated at 37°C for the next three days.…”