Comparative Evaluation of Tubex T F (Inhibition Magnetic Binding Immunoassay) for Typhoid Fever in Endemic Area

Khanna, Ashish K.; Khanna, Menka; Gill, Karamjit S.

doi:10.7860/jcdr/2015/15459.6810

Cited by 8 publications

(8 citation statements)

References 13 publications

(2 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However despite our findings on Semi-quantitative slide and single-tube Widal test being quit promising, it contradicted the findings on these two Sub-Saharan countries as these countries reported poor performance. In India, TUBEX ® TF test was recommended for the diagnosis of acute stage of the disease in a clinical setting with 76% sensitivity and 96-99% specificity (Khanna et al, 2015). These results were in agreement with our findings on TUBEX ® TF.…”

Section: Discussionsupporting

confidence: 91%

“…A sera diagnostic test can be of diagnostic value if its limitations are well known. In endemic areas, the Widal test which is an easy inexpensive test with its known shortcomings has been reported to be of diagnostic value in unvaccinated individuals where blood culture cannot be obtained especially in developing countries (Khanna et al, 2015). Our study had several limitations.…”

Section: Discussionmentioning

confidence: 89%

“…Scoring system of the TUBEX ® TF test may have led to operator bias. The scoring system should be standardized and operator bias should be removed by taking the readings with the help of machine only (Khanna et al, 2015). The results were read by at least 3 independent observers (The PI and 2 qualified technical persons) to validate the results.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Performance of TUBEX® TF IgM Antibody Test Against Culture to Detect Typhoid Fever Among Hospitalized Patients in Nairobi County

Lelei¹,

Nyerere²,

Onsare³

et al. 2019

JBAH

View full text Add to dashboard Cite

Typhoid fever has remained endemic and a major health problem in developing countries like Kenya due to poor sanitation, overcrowding and limited access to diagnostic services. The main aim of this study was to determine the diagnostic value of TUBEX ® TF IGM antibody tests for early diagnosis of typhoid fever among hospitalized patients in Nairobi County. TUBEX ® TF is a competitive immunoassay which detects presence of anti-09 IgM Salmonella Typhi antibodies in a patient's serum. We studied TUBEX ® TF a rapid sera diagnostic test for its usefulness in early diagnosis of typhoid fever and we compared its sensitivity and specificity to that of Widal test. The study was conducted on 92 (Gp-I) febrile patients who had clinically suggestive signs and symptoms of typhoid fever. Two groups of controls were created; 45 (Gp-II) and 15 (Gp-III) age and sex matched febrile and healthy controls, respectively. Blood culture was performed in all cases while TUBEX ® TF and Widal tests were performed in both cases and controls. The sample size was based on convenience at the two health facilities of our study. Gp-I had 9 (9.78%) blood culture positives for S.Typhi, 11(11.99%) were positive for TUBEX ® TF while 17 (18.48%) were positive for Widal test. 3 (6.67%) Gp-II were positive on both TUBEX ® TF and Widal test while none of the two tests tested positive on Gp-III. Among 9 culture positive cases, TUBEX ® TF was positive in 8 cases same as Widal test with sensitivity, specificity,

show abstract

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Performance of TUBEX® TF IgM Antibody Test Against Culture to Detect Typhoid Fever Among Hospitalized Patients in Nairobi County

Lelei¹,

Nyerere²,

Onsare³

et al. 2019

JBAH

View full text Add to dashboard Cite

show abstract

“…There are many new serological tests but most suffer from the same poor specificity as the Widal test and are more expensive . In India, Typhidot and TubexM – both serological tests detecting antibody against 50kD outer membrane protein and LPS, respectively – have been used in some laboratories but have similar sensitivity and specificity to the Widal test .…”

Section: Problems With Diagnosismentioning

confidence: 99%

Enteric fever in India: current scenario and future directions

Divyashree

Nabarro

Veeraraghavan

et al. 2016

Tropical Med Int Health

View full text Add to dashboard Cite

SummaryEnteric fever is a major cause of morbidity and mortality in tropical areas worldwide. The Indian subcontinent bears the brunt of the disease, both in terms of absolute case numbers and drugresistant strains. Recent phylogenetic studies suggest that the multidrug-resistant clade H58 originated in India and subsequently expanded through Asia and Africa. In Africa, it caused unrecognised outbreaks in areas previously considered free of the disease. In this study, we discuss the current status of enteric fever in India, the factors preventing its control and its future directions in this rapidly developing nation.

show abstract

“…The results calculated with a visual interpretation of the standard colors found on the Tubex TF kit. Score 0-10 on the color scale (zero according to the absence of specific antibody infections in patient's serum) (Khanna, Khanna, & Gill, 2015).…”

Section: Figure 1 Widal Results Examinationmentioning

confidence: 99%

The Comparison of Widal Slide Examination Results between Tubex TF on Febrile Observation Patients Over 3 Days

Cahyariza

Sholihah

2019

Med.Lab.Tech.J.

View full text Add to dashboard Cite

Typhoid fever is systematic bacterial disease usually occurs and has a high mortality rate each year, a disease transmitted from person to person due to contamination of feces, food, and water. The cause is bacterium Salmonella enterica serovar Typhi (S. Typhi) which is a natural host and reservoir for human. The limitations of the diagnostic test led to the increasing mortality rate due to typhoid fever. Besides ensuring infection in individuals, accurate serological tests are needed to ascertain the actual burden of the disease. Serological tests which are usually carried out in Puskesmas and hospital are Widal and Tubex Tf examinations. This study aims to determine whether there are differences in Widal and Tubex TF serological examinations in febrile patients over three days non-typhoid so patients can immediately find out whether they have typhoid fever or not. This study used a laboratory exploration method by examining 24 samples using Widal TYDAL and TUBEX® TF IDL Biotech. As many as 24 samples were examined by widal with antisera O, H, AH, and BH. Twenty-four of the same samples analyzed by TUBEX® TF. Results comparison of diagnostic from both methods will be compared using Mc Nemar test with significance = 0.05. Based on the examination which had done, it showed the difference in the results of Widal slide and lg M Anti Salmonella (Tubex Tf) in patients with febrile observation over three days. So, it can conclude that Tubex Tf examinations were better that widal slide examination because Tubex Tf uses Salmonella typhi anti-O9 antigen which can distinguish these organisms from >99% other Salmonella bacteria serotypes so that Tubex Tf examination is more specific.

show abstract

Comparative Evaluation of Tubex T F (Inhibition Magnetic Binding Immunoassay) for Typhoid Fever in Endemic Area

Cited by 8 publications

References 13 publications

Performance of TUBEX® TF IgM Antibody Test Against Culture to Detect Typhoid Fever Among Hospitalized Patients in Nairobi County

Performance of TUBEX® TF IgM Antibody Test Against Culture to Detect Typhoid Fever Among Hospitalized Patients in Nairobi County

Enteric fever in India: current scenario and future directions

The Comparison of Widal Slide Examination Results between Tubex TF on Febrile Observation Patients Over 3 Days

Contact Info

Product

Resources

About