Comparative evaluation of the MGITTM and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk

Grant, Irene R.; Kirk, R.B.; Hitchings, Edward; Rowe, M.T.

doi:10.1046/j.1365-2672.2003.02003.x

Cited by 48 publications

(29 citation statements)

References 17 publications

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“…Although the purpose of this study was not to assess if this system can be used for M. avium subsp. paratuberculosis identification in fecal samples, it is worth noting that a nonradiometric broth-based system (20,41) provides an easier, safer, and more cost-effective method for detection of mycobacteria than conventional culturing on solid media.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection and Typing of Strains ofMycobacterium aviumsubsp.paratuberculosisfrom Broth Cultures

et al. 2005

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A liquid culture followed by molecular confirmation was evaluated for potential to improve sensitivity and reduce time to diagnosis of Mycobacterium avium subsp. paratuberculosis infection. Fecal samples from 240 animals from Ohio farms were assessed for presence of M. avium subsp. paratuberculosis using four different protocols: (i) sedimentation processing followed by inoculation on Herrold's Egg Yolk media (HEYM) slants (monitored biweekly up to 16 weeks), (ii) double centrifugation processing followed by inoculation on HEYM slants (monitored biweekly up to 16 weeks), (iii) liquid-solid double culture method using modified 7H9 broth (8 weeks) followed by subculture on HEYM slants (monitored up to 8 weeks), and (iv) liquid culture using modified 7H9 broth (8 weeks) followed by molecular assays for the presence of two M. avium subsp. paratuberculosis-specific targets. The number of positive samples detected by each protocol was 37, 53, 65, and 76, respectively. Twenty-seven samples were positive by all four methods. Based on samples positive by at least one method (n ‫؍‬ 81), the sensitivities for sedimentation processing, double centrifugation processing, liquid-solid double culture, and liquid culture followed by molecular confirmation were 46%, 65%, 80%, and 94%, respectively. Fingerprinting of the positive samples using two polymorphic (G and GGT) short sequence repeat regions identified varying levels of within-farm and between-farm diversity. Our data indicate that liquid culture followed by molecular confirmation can significantly improve sensitivity and reduce time-to-diagnosis (from 16 to 8 weeks) of M. avium subsp. paratuberculosis infection and can also be efficiently employed for the systematic differentiation of M. avium subsp. paratuberculosis strains to understand the epidemiology of Johne's disease.

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Section: Discussionmentioning

confidence: 99%

Rapid Detection and Typing of Strains ofMycobacterium aviumsubsp.paratuberculosisfrom Broth Cultures

et al. 2005

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“…In other studies, both types of culture media were used for the detection of MAP and the obtained results were compared (Tables 6 and 7). Grant et al (2003) compared the detection capabilities of the non-radiometric MGIT System and radiometric BACTEC Culture System with UHT milk samples spiked with different levels of MAP (from 10 1 to 10 7 cells/ml) which were inoculated into MGIT and BACTEC media with or without chemical decontamination before culture. The MGIT system was found to be very effective and could be used as a replacement for the radiometric system to detect MAP in pasteurized milk.…”

Section: Culture Examination On Solid and In Liquid Mediamentioning

confidence: 99%

Detection methods for Mycobacterium avium subsp paratuberculosis in milk and milk products: a review

Slaná¹,

Paolicchi²,

Janštová³

et al. 2008

Vet. Med. - Czech

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Mycobacterium avium subsp. paratuberculosis (MAP) is the etiologic agent of paratuberculosis, a disease with considerable economic impact, principally on dairy cattle herds. Animals with paratuberculosis shed viable MAP especially in their milk, faeces and semen. MAP may have a role in the development of Crohn's disease in humans via the consumption of contaminated milk and milk products. The current methods of milk pasteurization are not sufficient to kill all MAP cells present in milk and MAP has been cultured from raw or pasteurized milk and isolated from cheese. The purpose of the present study was to review the different methods used for detection of MAP in milk and milk products. We analyze the current methods for direct or non direct identification of MAP and culture and molecular biology methods that can be applied to milk and milk products.

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“…MGITs have been successfully used for the detection and culture of M. avium subsp. paratuberculosis from animal tissues (8,11), milk (15), and breast milk and gut tissues of Crohn's disease patients (30,34). The quantitative capabilities of the Bactec MGIT 960 system have been previously explored only for C-type M. avium subsp.…”

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confidence: 99%

Quantification of Mycobacterium avium subsp. paratuberculosis Strains Representing Distinct Genotypes and Isolated from Domestic and Wildlife Animal Species by Use of an Automatic Liquid Culture System

et al. 2012

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bQuantification of 11 clinical strains of Mycobacterium avium subsp. paratuberculosis isolated from domestic (cattle, sheep, and goat) and wildlife (fallow deer, deer, wild boar, and bison) animal species in an automatic liquid culture system (Bactec MGIT 960) was accomplished. The strains were previously isolated and typed using IS1311 PCR followed by restriction endonuclease analysis (PCR-REA) into type C, S, or B. A strain-specific quantification curve was generated for each M. avium subsp. paratuberculosis strain by relating the time to detection in the liquid culture system to the estimated log 10 CFU in each inoculum. According to their growth curves, the tested M. avium subsp. paratuberculosis strains were classified into two distinct groups. The first group included the S-type strain isolated from goat and all the sheep strains with C, S, and B genotypes. A second group contained the C-and B-type strains isolated from cattle, goat, and wildlife animals with the exception of the fallow deer strain. The strains isolated from cattle or sheep showed similar strain-specific standard curves irrespective of their genotype. In contrast, the strains isolated from goat or from wildlife animal species varied in their rates of growth in liquid culture. Universal-standard curves and algorithms for the quantification of each group of strains were generated. In addition, the liquid culture system was compared with a real-time quantitative PCR system for the quantification of the 11 M. avium subsp. paratuberculosis strains. Correlations between the estimated log 10 CFU and M. avium subsp. paratuberculosis DNA copy numbers were very high for all the tested strains (R > 0.9).

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Comparative evaluation of the MGITTM and BACTEC culture systems for the recovery of Mycobacterium avium subsp. paratuberculosis from milk

Cited by 48 publications

References 17 publications

Rapid Detection and Typing of Strains ofMycobacterium aviumsubsp.paratuberculosisfrom Broth Cultures

Rapid Detection and Typing of Strains ofMycobacterium aviumsubsp.paratuberculosisfrom Broth Cultures

Detection methods for Mycobacterium avium subsp paratuberculosis in milk and milk products: a review

Quantification of Mycobacterium avium subsp. paratuberculosis Strains Representing Distinct Genotypes and Isolated from Domestic and Wildlife Animal Species by Use of an Automatic Liquid Culture System

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