Comparative evaluation of the diagnostic performance of autofluorescence and diffuse reflectance in oral cancer detection: a clinical study

Jayanthi, J.L.; Subhash, Narayanan; Stephen, Manju; Philip, Emmanuel K.; Beena, VT

doi:10.1002/jbio.201100037

Cited by 34 publications

(26 citation statements)

References 26 publications

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“…It has been reported that by analyzing the ratio of diffuse reflectance intensity in the oral mucosa at 545 nm and 575 nm, it is possible to distinguish between healthy and cancerous oral lesions due to changes in the spectra at these wavelengths, which are associated with oxygenated hemoglobin in the tissue [34][35][36][37]. While a standard RGB camera is unable to differentiate between these two wavelengths, the IMS allows for a clear distinction.…”

Section: Imaging Of Ex Vivo Oral Tissuementioning

confidence: 99%

Development of a multimodal foveated endomicroscope for the detection of oral cancer

Shadfan

Darwiche

Blanco

et al. 2017

Biomed. Opt. Express

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A multimodal endomicroscope was developed for cancer detection that combines hyperspectral and confocal imaging through a single foveated objective and a vibrating optical fiber bundle. Standard clinical examination has a limited ability to identify early stage oral cancer. Optical detection methods are typically restricted by either achievable resolution or a small field-of-view. By combining high resolution and widefield spectral imaging into a single probe, a device was developed that provides spectral and spatial information over a 5 mm field to locate suspicious lesions that can then be inspected in high resolution mode. The device was evaluated on ex vivo biopsies of human oral tumors. M. Gillenwater, "Accuracy of in vivo multimodal optical imaging for detection of oral neoplasia," Cancer Prev.

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Section: Imaging Of Ex Vivo Oral Tissuementioning

confidence: 99%

Development of a multimodal foveated endomicroscope for the detection of oral cancer

Shadfan

Darwiche

Blanco

et al. 2017

Biomed. Opt. Express

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“…Considerable work has been carried out by many groups during the last several years to develop the technique of tissue fluorescence spectroscopy as a suitable method for early diagnosis of oral cancer [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. Almost all these studies were done on buccal mucosa, though other sites in the oral cavity, like tongue, lips, and upper and lower palates, also are often found to be sites of malignancy.…”

Section: Introductionmentioning

confidence: 99%

“…Proper interpretation of the observed fluorescence spectra of these different sites is thus necessary for, not only to develop suitable optical methods for diagnosis, but also to understand the complex biological processes that take place during induction, progression, and regression of oral malignancy, Non-Invasive in vivo Screening of Oral Malignancy Using Laser-Induced Fluorescence Based System and follow up in therapy. In earlier studies on fluorescence spectra of different sites in the oral cavity mostly spectra above 450 nm have only been recorded, and excitation wavelengths have also been limited to 337 nm and above [6,9,16,20].…”

Section: Introductionmentioning

confidence: 99%

“…The problem is more acute when pulsed lasers are used for excitation, and spectra are recorded after some delay from the laser pulse, since a few nanoseconds jitter in the laser or intensified charge-coupled device (ICCD) trigger and gate, can give spectra which may vary from time to time, due to fast decay of the fluorescence of some of the species [17]. Some workers have used excitation wavelengths around 400 nm and have shown that fluorescence in the 500-700 nm region, from porphyrins, is capable of discrimination between normal and malignant conditions [6,20]. However, this difference presumably from increased angiogenesis in malignancy, may lead to misleading results, since conditions like inflammation of tissue, wound healing, and bacterial invasion produce similar situations.…”

Section: Introductionmentioning

confidence: 99%

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Non-Invasive in vivo Screening of Oral Malignancy Using Laser-Induced Fluorescence Based System

Patil¹,

Unnikrishnan²,

Ongole

et al. 2018

Sovrem Tehnol Med

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About 4000 spectra from oral cavity sites have been recorded in vivo from 380 volunteers, under normal (n=133), potentially malignant (n=155), and malignant (n=92) conditions, by excitation with 325 nm CW He-Cd laser. The analyses of the spectra show that the overall spectra arise from about 7-8 bands from different molecular species. In healthy condition (from subjects with no abnormal oral conditions, including tobacco use), buccal mucosa, lip underside, and tongue bottom give spectra very similar to each other, while tongue top, tongue lateral, and palate give spectra different from these as well as from each other. Under potentially malignant and malignant conditions all sites give spectra which are noticeably different for different sites and under different conditions. It is therefore advisable to use separate reference data sets of normal, potentially malignant, or malignant conditions for different sites for optical diagnostic applications.

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“…The variations in the relative distribution of NADH and porphyrins in the emission spectra corresponding to 405 nm excitation during the tissue transformation process may be due to (i) in the early weeks of DMBA initiation, the inflammatory response is triggered in the skin, thereby increasing the metabolic activity of the inflammated cells, leading to increased level of NADH in the early tissue transformation conditions namely hyperplasia, papilloma and dysplastic lesions,28 and (ii) during the degree of differentiation increases due to chronic application of DMBA in the mouse skin; the NADH decreases leading to an increased level of porphyrin in the malignant transformation of ESCC and WDSCC lesions 29,30. However, with an increase in the degree of malignancy as in the case of both ESCC and WDSCC lesions, the NADH level decreased in intensity compared with hyperplasia, papilloma and dysplasia lesions.…”

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confidence: 99%

Native fluorescence spectroscopic characterization of DMBA induced carcinogenesis in mice skin for the early detection of tissue transformation

Ebenezar

Aruna

Ganesan

2015

Analyst

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The objective of the study is to characterize the endogenous porphyrin fluorescence in a dimethylbenz(a)anthracene (DMBA) induced mouse skin tumor model using native fluorescence emission and excitation spectroscopy. Two intensity ratio parameters I580/I635 and I420/I515 were selected to represent the key fluorophore of endogenous porphyrins from emission and excitation spectra recorded in vivo from 31 DMBA treated animals and 5 control animals. In the emission spectrum, the endogenous porphyrin was elevated at 635 nm in different transformation lesions such as hyperplasia, papilloma, dysplasia, ESCC and WDSCC. This is corroborated by the endogenous porphyrin elevation at 420, 515, 550 and 588 nm in the WDSCC lesions from the excitation spectra. The elevation of endogenous porphyrin, probably protoporphyrin IX (PpIX), is due to biochemical and metabolic alterations in epithelial cells during tissue transformation. The loss of ferrochelatase activity might be responsible for enhanced PpIX in the transformed tissues. The sensitivity and specificity were determined for different lesion pairs from the scatter plot based on the discrimination value by validation with histopathological results. The emission intensity ratio I580/I635 at 405 nm excitation was selected to discriminate normal from hyperplasia, hyperplasia from papilloma, papilloma from dysplasia, dysplasia from early squamous cell carcinoma (ESCC), and ESCC from well differentiated squamous cell carcinoma (WDSCC) with specificities of 100%, 88%, 100%, 86%, and 100% and sensitivities of 100%, 80%, 100%, 100% and 100% respectively. Similarly, the excitation intensity ratio I420/I515 for 635 nm emission used to discriminate between WDSCC lesions and normal tissue gives 100% specificity and 100% sensitivity.

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Comparative evaluation of the diagnostic performance of autofluorescence and diffuse reflectance in oral cancer detection: a clinical study

Cited by 34 publications

References 26 publications

Development of a multimodal foveated endomicroscope for the detection of oral cancer

Development of a multimodal foveated endomicroscope for the detection of oral cancer

Non-Invasive in vivo Screening of Oral Malignancy Using Laser-Induced Fluorescence Based System

Native fluorescence spectroscopic characterization of DMBA induced carcinogenesis in mice skin for the early detection of tissue transformation

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