Comparative Evaluation of the Cytological, Histopathological and Immunohistochemical Findings of Canine Cutaneous and Subcutaneous Masses

Abstract

In this study, we compared the cytological, histopathological, and immunohistochemical diagnoses of 71 canine cutaneous and subcutaneous masses. Cytological diagnoses included 56 tumors (21 mesenchymal, 15 epithelial, 16 round cell, four melanocytic), 13 inflammatory reactions, and two cysts. Of the 21 cytologically diagnosed mesenchymal tumors, three were later confirmed non-tumoral (hematoma, granulation tissue, fibroepithelial polyp). Thirteen out of 15 epithelial tumors were correctly diagnosed cytologically, whereas two cases were confirmed to be non-tumoral (fibroepithelial polyp, granulation tissue) after histopathological examination. One mast cell tumor was later confirmed as fibrous hyperplasia; diagnoses were correct in other round cell tumors. Cytological diagnoses were correct for all melanocytic tumors and cystic lesions. Five cases which had been cytologically diagnosed as inflammatory reactions were diagnosed as tumors (lymphoma, papilloma, sebaceous adenoma, and squamous cell carcinoma) after histopathological examination. Immunohistochemistry confirmed the histopathological diagnoses of all epithelial and round cell tumors, while the diagnoses of six mesenchymal tumors were changed after the immunohistochemical examination. The total accuracy of cytology in the diagnosis of tumoral/non-tumoral masses was 84.5%, and the accuracy in the determination of benign/malignant behavior was 83%. Diagnostic accordance between histopathology and immunohistochemistry was 86.6%. High success rates obtained with cytological diagnoses prove that cytology is a reliable diagnostic tool. The main diagnostic challenge remains with mesenchymal tumors and tumors accompanied by inflammatory reactions. The results suggest that immunohistochemistry is fundamental for diagnoses of most mesenchymal tumors.

Spontaneous Lymphoma in a SMP30 Knock-Out C57BL/6 Mouse

“…Following immunohistochemistry, analysis using Histostain® Plus Broad-Spectrum Kit (Cat#859043, Life Technologies, Carlsbad, CA, USA) revealed complete loss of SMP30 (COSMOBIO CO., LTD., SML-RO1001-EX, 1:500, Tokyo, Japan) in SMP30 KO mouse (Figure 3 A and B). Additionally, the neoplastic round cells exhibited strong positive responses for CD3 (Agilent, Santa Clara, A0452, 1:200, Santa Clara, USA) which is a major marker of T-lymphocytes (Figure 3 C and D) [17]. The differential diagnosis of the present case included mast cell tumor, histiocytic sarcoma, and plasma cell tumor.…”

Effects of dietary supplementation with benzoic acid and chelated copper, zinc and manganese sources on production performance in piglets

“…After fi xation the samples were embedded in paraffi n wax and then were cut into 5 µm transverse sections for staining. The tissue sections were stained by hematoxylin and eosin (HE) and periodic shiff acid (PAS) stain [31]. Intestinal mucosal morphology including villus height, villus width and crypt depth was determined with an Olympus BX-51 microscope.…”