In this study, antibacterial activity of ethyl acetate extracts of Agaricus bisporus fruiting bodies and ethyl acetate extracts of Albizzia lebbeck leaves were examined in-vitro with (2) two pathogenic bacteria (Escherichia coli and Proteus) and yeast (Candida albicanus), following agar well diffusion method using different concentrations (25, 50, and 100μl). The extracts of Agaricus bisporus and Albizzia lebbeck were showed potent antimicrobial activity against tested bacteria and yeast. Agaricus bisporus fruiting bodies extract was showed the highest inhibitory effect versus growth of bacteria and yeast were tested in this study. The E. coli and Proteus were found to show large sensitivity to the extracts of Agaricus bisporus with 12 and 13mm inhibition zones respectively at 25 μl concentration while Candida albicanus was more resistant to this extract with inhibition zone of 9mm at 25 μl concentration. In the same time the inhibition zone of Albizzia lebbeck against E coli, Proteus and Candida albicanus were 12, 10.8, 6mm respectively at 25 μl concentration.