“…The 5′-nucleotides(GMP, IMP, and AMP) were extracted and analyzed on a HPLC system (UltiMate 3000, Thermo Fisher Scientific Co., Ltd., Massachusetts, USA), according to the method modified from Wen et al (2020) . A chromatographic column (Acclaim PolarAdvantage II C18, 50 mm × 4.6 mm, 3 μm) with mobile phase A of methanol and mobile phase B of 20 mM KH 2 PO 4 -K 2 HPO 4 buffer solution (v/v = 1:1, pH = 5.8).…”