Comparative evaluation of proliferative potential and replicative senescence associated changes in mesenchymal stem cells derived from dental pulp and umbilical cord

Das, Monalisa; Das, Ankita; Barui, Ananya; Paul, Ranjan Rashmi

doi:10.1007/s10561-021-09926-8

Cited by 7 publications

(3 citation statements)

References 50 publications

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“…This is consistent with a previous study that compared senescence in dental pulp-derived MSCs (DPSCs) and UCSCs at passage 6 and found significantly higher SA-β-gal expression in UCSCs. 30 Interestingly, despite UCSCs being assumed to be the youngest MSCs, our study showed that the ability of infant ADSCs to delay senescence was stronger than that of UCSCs.…”

Section: Discussionmentioning

confidence: 61%

Multilineage differentiation potential in the infant adipose-and umbilical cord-derived mesenchymal stem cells

Huang,

Hsueh,

Liao

et al. 2023

Journal of the Chinese Medical Association

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This study aims to compare the biological properties of infant adipose-derived mesenchymal stem cells (infant ADSCs) from excised polydactyly fat tissue and umbilical cord-derived mesenchymal stem cells (UCSCs) in terms of proliferation and differentiation capabilities. The proliferation of infant ADSCs and UCSCs was analyzed by determining the fold changes of cell numbers and doubling time periods. The state of senescence and replicative stress was compared by analyzing the expression of age-related genes, senescence associated β-galactosidase (SA-β-gal) staining, and phosphorylated histone variant H2AX (γH2AX) immunofluorescence staining. The expression levels of superoxide dismutase (SODs) and genes related to multilineage differentiation were analyzed using quantitative reverse transcription polymerase chain reaction (RT-qPCR). Differentiation levels were determined using histochemical staining, immunohistochemical staining, and immunofluorescence staining. Infant ADSCs exhibited higher proliferation rates and expression levels of SOD1, SOD2 and SOD3 at passages 3-5 compared with UCSCs. Senescence related genes (p16, p21 and p53), SA-β-gal staining, and replicative stress analysis were reduced in infant ADSCs. The expression levels of chondrogenic genes (COL2 and COL10), osteogenic genes (RUNX2 and ALP), adipogenic genes (LPL), and hepatogenic genes (ALB and TAT) in infant ADSC-differentiated cells were significantly higher than those in UCSCs. Histochemical and immunofluorescence staining confirmed these results. Only the expression levels of tenogenic genes (MMP3, DCN, and COL3) in infant ADSC-differentiated cells were lower than those in UCSCs. Infant ADSCs exhibit higher proliferation rates, reduced cellular senescence and replicative stress, better antioxidative activity, and higher differentiation potential toward chondrogenic, osteogenic, adipogenic and hepatogenic lineages than UCSCs.

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Section: Discussionmentioning

confidence: 61%

Multilineage differentiation potential in the infant adipose-and umbilical cord-derived mesenchymal stem cells

Huang,

Hsueh,

Liao

et al. 2023

Journal of the Chinese Medical Association

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“…According to the findings, the source of stem cells determines both MSCs proliferative capacity and replicative senescence. As a result, this work will pave the way for future investigations into choosing acceptable stem cell sources for regenerative therapeutic applications [ 217 ].…”

Section: Umbilical Cord Stem Cellsmentioning

confidence: 99%

Human Umbilical Cord Mesenchymal Stem Cells: Current Literature and Role in Periodontal Regeneration

Shaikh

Shahzad

Tash

et al. 2022

Cells

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Periodontal disease can cause irreversible damage to tooth-supporting tissues such as the root cementum, periodontal ligament, and alveolar bone, eventually leading to tooth loss. While standard periodontal treatments are usually helpful in reducing disease progression, they cannot repair or replace lost periodontal tissue. Periodontal regeneration has been demonstrated to be beneficial in treating intraosseous and furcation defects to varied degrees. Cell-based treatment for periodontal regeneration will become more efficient and predictable as tissue engineering and progenitor cell biology advance, surpassing the limitations of present therapeutic techniques. Stem cells are undifferentiated cells with the ability to self-renew and differentiate into several cell types when stimulated. Mesenchymal stem cells (MSCs) have been tested for periodontal regeneration in vitro and in humans, with promising results. Human umbilical cord mesenchymal stem cells (UC-MSCs) possess a great regenerative and therapeutic potential. Their added benefits comprise ease of collection, endless source of stem cells, less immunorejection, and affordability. Further, their collection does not include the concerns associated with human embryonic stem cells. The purpose of this review is to address the most recent findings about periodontal regenerative mechanisms, different stem cells accessible for periodontal regeneration, and UC-MSCs and their involvement in periodontal regeneration.

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“…The current method for collecting and preserving DPSCs is to collect pulp tissue from extracted teeth, culture the growing cells until they reach subconfluency, and cryopreserve them [23,[27][28][29]. This method has a variety of problems, however, including the need for a long culture period before cell collection [24,30], a high risk of contamination [31][32][33][34], high cost [34], and the need to secure storage space for cryopreservation [23,35]. Therefore, it is necessary to establish a low-cost, simple, and reliable cell collection system to procure and stably secure more DPSCs for the efficient implementation of regenerative medicine [27,29].…”

Section: Introductionmentioning

confidence: 99%

Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

Takeshita-Umehara,

Tokuyama-Toda,

Takebe

et al. 2023

Cells

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Dental pulp stem cells (DPSCs) are considered a valuable cell source for regenerative medicine because of their high proliferative potential, multipotency, and availability. We established a new cryopreservation method (NCM) for collecting DPSCs, in which the tissue itself is cryopreserved and DPSCs are collected after thawing. We improved the NCM and developed a new method for collecting and preserving DPSCs more efficiently. Dental pulp tissue was collected from an extracted tooth, divided into two pieces, sandwiched from above and below using cell culture inserts, and cultured. As a result, the cells in the pulp tissue migrated vertically over time and localized near the upper and lower membranes over 2–3 days. With regard to the underlying molecular mechanism, SDF1 was predominantly involved in cell migration. This improved method is valuable and enables the more efficient collection and reliable preservation of DPSCs. It has the potential to procure a large number of DPSCs stably.

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Comparative evaluation of proliferative potential and replicative senescence associated changes in mesenchymal stem cells derived from dental pulp and umbilical cord

Cited by 7 publications

References 50 publications

Multilineage differentiation potential in the infant adipose-and umbilical cord-derived mesenchymal stem cells

Multilineage differentiation potential in the infant adipose-and umbilical cord-derived mesenchymal stem cells

Human Umbilical Cord Mesenchymal Stem Cells: Current Literature and Role in Periodontal Regeneration

Improved Method for Dental Pulp Stem Cell Preservation and Its Underlying Cell Biological Mechanism

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