Comparative evaluation of polymerase chain reaction assay with microscopy for detection of asymptomatic carrier state of theileriosis in a herd of crossbred cattle

Charaya, Gaurav; Rakha, N. K.; Maan, Sushila; Kumar, Aman; Kumar, Tarun; Jhambh, Ricky

doi:10.14202/vetworld.2016.1039-1042

Cited by 18 publications

(8 citation statements)

References 11 publications

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“…Our result recorder 88% rate by PCR and 39.33% microscopically, these agreed with many researchers, (28) in Turkey and (29) in India, the differences between microscopic and molecular methods attributed to several reasons as chronic and carrier cases with low parasitemia and parasite misdiagnosis with staining artifacts (30). Phylogenetic analysis confirmed low differences between Iraqi strains of Theileria annulata and other countries .…”

Section: Resultssupporting

confidence: 91%

Molecular surveillance and phylogenetic analysis of Theileria annulata in bovine at Baghdad city/ Iraq

Kawan

2019

Iraqi J. Vet. Med.

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This study is conducted to investigate Theileria spp. by traditional and molecular methods. A total of 150 blood and 50 lymph samples were collected from local breed symptomatically and asymptomatically cattle of both sexes with age ranging from less than 6 months to more than 1 year during the four seasons of 2018, in different parts at Baghdad city / Iraq. Microscopic examination of Giemsa stained blood smears revealed 39.33 %( 59/150) rate of infection with bovine theileriosis and 34 %( 17/50) positive lymph smears. Statistically no significant difference recorded between female and male: 42.04 % (37/88) and 35.48 % (22/62) respectively. Higher rate of infection 57.97 % (40/69) were recorded in more than 1 year age and 0 % in less than 6 months. 48.93 % (23/47) rate of bovine theileriosis was recorded during summer and 39.53 % (17/43) ; 37.5 % (15/40) rates were recorded during spring and autumn respectively, while the lower rate recorded in winter 20 % (4/20). DNA extraction and polymerase chain reaction (conventional PCR) were done on all cattle blood samples the result recorded that 22 out of 25 samples were positive for Theileria spp and Theileria annulata with percentage of 88 %. Also DNA sequencing analysis and genetic relationship were conducted by phylogenetic analysis.

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Section: Resultssupporting

confidence: 91%

Molecular surveillance and phylogenetic analysis of Theileria annulata in bovine at Baghdad city/ Iraq

Kawan

2019

Iraqi J. Vet. Med.

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“…As well as, PCR is much more sensitive in clinically infected, apparently healthy animals (carriers) and in the early phase of infection. This agree with Charaya et al (2016) reported that PCR assay showed no cross-reactivity with theileria sp. and can be used specifically to diagnose theileriosis in herd and differentiate it from other hemoprotozoan diseases.…”

Section: Discussionsupporting

confidence: 93%

Detection And Molecular Characterization of Theileria Ovis in Sheep And Goats with Clinical The Ileriosis in Kurdistan, Iraq

Hassen

Meerkhan

2020

UOD

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This study was carried out to detect Theileria infection in sheep and goats in Kurdistan region, Iraq from June 2019 to April 2020. Molecular method was used to identify Theileria species. Sixty- seven blood samples were taken from 45 sheep and 22 goats based on clinical signs of theileriosis during tick activating season. The 67 samples were PCR edm and as a result, 20 species-specific PCR were positives (26.67% (12/20) were Theileria ovis in sheep and 36.36% (8/20) were from goats). The results of the gene analysis in the current study were registered in NCBI under four accession numbers (MN889986, MN889987, MN889988 and MN889989), which shows that sheep and goats can be infected with Theileria ovis. This is the first report of Theileria species in goats with clinical theileriosis in Kurdistan, so the gene flow and disease transmission between sheep and goats is most expected. PCR is a useful diagnostic tool to detect ovine theileriosis with a single test and suggested that T. ovis is the dominant piroplasmid agent in Erbil. In addition, it revealed that sheep is very susceptible to theileriosis than goats

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“…As shown in Table 2, DreamTaq Green PCR master mix (2x) was cheapest of Taq polymerase. This Taq polymerase further has been reported to also be applied for some PCR routine check application (Charaya et al 2016). However, as one of the commercial Taq polymerases, DreamTaq Green PCR master mix (2x) is susceptible to DNA contamination.…”

Section: Discussionmentioning

confidence: 99%

Low cost and comprehensive pork detection in processed food products with a different food matrix

Sugiana

Widyowati

Warisman

et al. 2018

Indones. J. Biotechnol.

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The adultera on of processed beef-based meat products with pork is a sensi ve issue in Indonesia. Therefore a simple, low cost, and accurate method is required for the detec on of pork, so as to protect consumers from accidental consump on of adulterated meat. In this study, we developed a detec on method for the low cost iden fica on of pork in processed meat products. We used the cost-efficient Taq DNA polymerase, DreamTaq Green PCR master mix (2x), and duplex PCR method to recognize pork simultaneously with 18S rRNA detec on. A posi ve control containing a pork gene inserted into pGEM ® -T easy was prepared, along with a nega ve control. The results of the duplex PCR were used to assess its specificity, detec on limit, and its ability to recognize pork in processed meat products with a different food matrix. 18S rRNA detec on was for confirming DNA integrity of DNA extracted from the processed food, while the posi ve control confirmed that the reagents were working well and the nega ve control confirmed a non-contamina on problem. Following this, the duplex PCR was op mized and the op mum concentra on primer for duplex PCR detec on was found to be 3 µM for pork and 0.2 µM for 18S rRNA. As li le as 3.125 ng of the DNA template could be used to detect whether a sample contained pork. Of the nine commercial processed meat products tested, five were found to contain pork while four halal products showed no signs of pork. It can be concluded that duplex PCR is a simple, fast, sensi ve, specific, and low cost method of detec ng pork in processed meat products.

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Comparative evaluation of polymerase chain reaction assay with microscopy for detection of asymptomatic carrier state of theileriosis in a herd of crossbred cattle

Cited by 18 publications

References 11 publications

Molecular surveillance and phylogenetic analysis of Theileria annulata in bovine at Baghdad city/ Iraq

Molecular surveillance and phylogenetic analysis of Theileria annulata in bovine at Baghdad city/ Iraq

Detection And Molecular Characterization of Theileria Ovis in Sheep And Goats with Clinical The Ileriosis in Kurdistan, Iraq

Low cost and comprehensive pork detection in processed food products with a different food matrix

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