Comparative evaluation of platelet augmentation activity of Carica papaya leaf juice and hydrocortisone in thrombocytopenic rats

Abstract

AimThe study aims at determining the possible effects of Carica papaya leaf juice and hydrocortisone in increasing the platelet count in thrombocytopenia similar to dengue fever. MethodologyThe study was carried out on 24 Long Evans Norwegian strain healthy rats to investigate the comparative platelet increasing effect of Carica papaya leaf juice and hydrocortisone in experimentally induced thrombocytopenic rats. Thrombocytopenia was induced by 3 doses of cyclophosphamide (100mg/kg body weight) given subcutaneously. The rats were divided into 4 groups comprising of six animals in each group. These groups included a normal control and a cyclophosphamide induced thrombocytopenic control and the remaining two cyclophosphamide induced thrombocytopenic groups were administered Carica papaya leaf juice and hydrocortisone respectively.The duration of study was 15 days. The clotting time was determined on the 15th day although blood samples were obtained from the rat tails at various time intervals to determine the platelet count.Collected data was tabulated and Statistical analysis was performed by using appropriate significant test. A probability value P < 0.05 was considered to be statistically significant. ResultAverage platelet counts of the test and control groups were (6.89)×10 5 /µL and (6.75)×10 5 /µL respectively before the experiment. In the experiment there was no significant difference in the platelet counts during the first 3 days in either group. However, platelet counts within the test group started to fall after Day 3 and remarkable thrombocytopenia developed after 7days and the average value was (2.68)×10 5 /µL. From day 11 Carica papaya leaf juice and Hydrocortisone was given in two test groups following cyclophosphamide and platelet counts observed to reach a peak level at Day 14 (7.83)×10 5 /µL in case of following Carica papaya leaf juice, and (4.05)×10 5 /µL in case of following Hydrocortisone. The clotting time was determined on day 15 and it was found to be considerably lower (95.5 ± 2.762 sec) in case of Carica papaya leaf juice than (129±3.124 sec) in Hydrocortisone. ConclusionCarica papaya leaf juice has more platelet increasing effect than hydrocortisone and this study also reflects that Carica papaya leaf juice may be a potential candidate for further research leading to the development of a herbal therapeutic agent for thrombocytopenia manifested in diseases such as dengue.

The Potential Role of Thrombopoietin and Interleukin-6 in the Thrombocytosis Effect of Carica papaya Leaves

“…We irst validated the thrombocytosis effect of CPL in vivo using chemotherapy induced rat model. This is consistent with others who demonstrated similar effect either using busulfan (Zunjar et al, 2016) or different bone marrow suppressant like cyclophosphamide (Patil et al, 2013;Akhter et al, 2015) and carboplatin (Tahir et al, 2014). Both aqueous and methanol extracts were signi icantly increased compared to controls with CPL aqueous with better ef icacy.…”

Effect of Carica papaya Leaf Juice on Bone Marrow Aspirate of Busulfan-induced Chronic Bone Marrow Aplasia in Mice

“…This study was performed to observe Carica papaya leaf juice effect on prevention of busulfan induced bone marrow aplasia in mice. Previous literature shows a range of factors that have been used for induction of bone marrow aplasia, among which are: benzene & chloramphenicol 14 , busulfan 10,15 and cyclophosphamide 16 . Busulfan was selected for this study as it causes irreversible damage to the bone marrow.…”

Effect of Carica papaya Leaf Juice on Blood Cell Count of Busulfan-Induced Chronic Bone Marrow Aplasia in Mice

“…7 Many fruitful researches on role of papaya leaf extract/juice in boosting up the platelet count, white blood cell (WBC) count and erythrocyte (RBC) count have been carried out. 8,9,10 These all studies are performed on normal animals, dengue patients or reversible models of myelosuppression. The current study was planned to explore the effect of Carica papaya leaf juice on prevention of irreversible myelosuppression induced by busulfan.…”