Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks

Goriya, Harshad V.; Kalia, Anil; Bhavsar, Shailesh K.; Joshi, Chaitanya; Rank, D. N.; Thaker, Aswin M.

doi:10.4142/jvs.2005.6.4.279

Cited by 16 publications

(17 citation statements)

References 16 publications

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“…However, chicken do not possess CAR or PXR, but chicken xenobiotic receptor (CXR) as an orthologous receptor to them [27]. In chicken, PB is known to induce CYP2C genes and CYP3A genes [10,11,28,29]. Our result also showed significant induction of CYP2C23a , CYP2C23b , CYP2C45 and CYP3A37 in female chicken, although only CYP2C45 was significantly induced in male chicken.…”

Section: Resultsmentioning

confidence: 65%

Avian Cytochrome P450 (CYP) 1-3 Family Genes: Isoforms, Evolutionary Relationships, and mRNA Expression in Chicken Liver

et al. 2013

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Cytochrome P450 (CYP) of chicken and other avian species have been studied primarily with microsomes or characterized by cloning and protein expression. However, the overall existing isoforms in avian CYP1-3 families or dominant isoforms in avian xenobiotic metabolism have not yet been elucidated. In this study, we aimed to clarify and classify all of the existing isoforms of CYP1-3 in avian species using available genome assemblies for chicken, zebra finch, and turkey. Furthermore, we performed qRT-PCR assay to identify dominant CYP genes in chicken liver. Our results suggested that avian xenobiotic-metabolizing CYP genes have undergone unique evolution such as CYP2C and CYP3A genes, which have undergone avian-specific gene duplications. qRT-PCR experiments showed that CYP2C45 was the most highly expressed isoform in chicken liver, while CYP2C23b was the most highly induced gene by phenobarbital. Considering together with the result of further enzymatic characterization, CYP2C45 may have a dominant role in chicken xenobiotic metabolism due to the constitutive high expression levels, while CYP2C23a and CYP2C23b can be greatly induced by chicken xenobiotic receptor (CXR) activators. These findings will provide not only novel insights into avian xenobiotic metabolism, but also a basis for the further characterization of each CYP gene.

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Section: Resultsmentioning

confidence: 65%

Avian Cytochrome P450 (CYP) 1-3 Family Genes: Isoforms, Evolutionary Relationships, and mRNA Expression in Chicken Liver

et al. 2013

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“…Induction occurs predominantly in the liver, intestine and, to a lower extent, in other extra‐hepatic tissues (e.g. kidney and lung: Denison & Whitlock, ; Kakizaki et al ., ; Goryia et al ., ). What's more, target gene up‐regulation is mostly driven by NRs (NR1I2 and NR1I3: Kodama & Negishi, ).…”

Section: Discussionmentioning

confidence: 97%

“…Thus, PB was (Dupuy et al ., ) and it is still used as a prototypical inducer useful to characterize DMEs expression (Longo et al ., ), NR‐dependant regulation phenomena (Tamasi et al ., ), as well as potential drug–drug interactions (Ballent et al ., ). By contrast, few papers investigating the in vivo effects of PB upon DMEs gene expression in veterinary species have been published so far and none in cattle (Kawalek et al ., ; Chirulli et al ., ; Goryia et al ., ; Marini et al ., ; Makino et al ., ).…”

Section: Discussionmentioning

confidence: 98%

“…Jones et al ., ; Zheng et al ., ) and, seldom, in the dog (Kawalek et al ., ; Fukunaga et al ., ). On the contrary, a single‐dose protocol was used in those in vivo studies where the effects of PB were investigated in veterinary species (Dupuy et al ., ; Chirulli et al ., ; Goryia et al ., ; Marini et al ., ). Altogether, as different PB concentrations, routes of administration and protocol duration were used, a clear‐cut comparison among species cannot be made; nevertheless, the presence of low PB concentrations in cattle tissues might hypothetically be offered as a further explanation for present conflicting results.…”

Section: Discussionmentioning

confidence: 98%

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Tissue distribution and phenobarbital induction of target SLC‐ and ABC‐ transporters in cattle

Zancanella

Giantin

Lopparelli

et al. 2012

Vet Pharm & Therapeutics

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In veterinary pharmaco-toxicological sciences, few data about uptake and efflux drug transporters (DTs) expression and regulation phenomena have been published. In this study, the tissue distribution and transcriptional modulation of solute carrier (SLC) and ATP-binding cassette (ABC) DTs were investigated in cattle orally administered with phenobarbital (PB) by using a quantitative real-time RT-PCR approach. The criterion for target gene selection was the PB-responsiveness in human and rodent model species. All target DTs were expressed in the liver. Only two of the seven PB-responsive target DTs (SLCO1B3 and SLC10A1) were not constitutively expressed in cattle extra-hepatic tissues. The greatest number of DTs (SLCO2B1, ABCB1, ABCC2, ABCG2) were expressed in intestine and testis, followed by, adrenal gland (SLCO2B1, ABCB1, ABCG2), lung (ABCB1, ABCG2), kidney, and skeletal muscle (ABCG2). PB administration never altered DTs mRNA levels, except for an increase in hepatic ABCC2 mRNA and a down-regulation of renal ABCG2. Altogether, these results confirm only to some extent data obtained in humans and laboratory species; clearly, they should be considered a preliminary step for further molecular investigations about species-differences in DT gene expression and regulation as well as in DT expression and function.

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“…Treatment with phenobarbital causes hepatic induction of transcription of a number of cytochrome P450 forms in chickens, including 2H1, 2H2 and 3A. (Walker, 1998; Goriya et al. , 2005).…”

Section: Pharmacokinetic Parameters For Phenobarbital Administered Omentioning

confidence: 99%

Pharmacokinetics of orally administered phenobarbital in African grey parrots (Psittacus erithacus erithacus)

Powers

Papich

2011

Journal of Veterinary Pharmacology and Therapeutics

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Comparative evaluation of phenobarbital-induced CYP3A and CYP2H1 gene expression by quantitative RT-PCR in Bantam, Bantamized White Leghorn and White Leghorn chicks

Cited by 16 publications

References 16 publications

Avian Cytochrome P450 (CYP) 1-3 Family Genes: Isoforms, Evolutionary Relationships, and mRNA Expression in Chicken Liver

Avian Cytochrome P450 (CYP) 1-3 Family Genes: Isoforms, Evolutionary Relationships, and mRNA Expression in Chicken Liver

Tissue distribution and phenobarbital induction of target SLC‐ and ABC‐ transporters in cattle

Pharmacokinetics of orally administered phenobarbital in African grey parrots (Psittacus erithacus erithacus)

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