Comparative evaluation of PAR1, GPIb-IX-V, and integrin αIIbβ3 levels in cord and adult platelets

Schlagenhauf, Axel; Schweintzger, Sabrina; Birner-Grünberger, Ruth; Leschnik, B.; Muntean, W.

doi:10.1055/s-0037-1619105

Cited by 10 publications

(10 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Platelet activation by TRAP resulted in marginal increases in P-selectin expression and PNA formation. This is consistent with other studies showing poor TRAP response of platelets at birth, due to low levels of the protease-activated receptors (PAR-1 and PAR-4) [45, 46]. Platelet responsiveness to TRAP was then observed to improve postnatally with age [24, 25, 46].…”

Section: Discussionsupporting

confidence: 91%

Platelet-Neutrophil Interactions Are Lower in Cord Blood of Premature Newborns

et al. 2018

View full text Add to dashboard Cite

Objective: To quantify platelet-neutrophil interaction by flow cytometry, in newborn cord blood, as a function of gestational age. Rationale: Little is known about platelet function markers in the newborn, and developmental variations in these markers are not well described. Methods: Cord blood samples were obtained from 64 newborns between 23 and 40 weeks’ gestation. The neonates were grouped into three categories: preterm (< 34 weeks’ gestation, n = 21), late preterm (34 to < 37 weeks’ gestation, n = 22), and term (≥37 weeks’ gestation, n = 21). We monitored the expression of P-selectin and the formation of platelet-neutrophil aggregates (PNAs) by flow cytometry while using adenosine 5′-diphosphate (ADP) or thrombin receptor-activating peptide (TRAP) as agonists. Results: PNAs were significantly lower in preterm compared to term neonates after TRAP or ADP stimulations (11.5 ± 5.2% vs. 19.9 ± 9.1%, p < 0.001, or 24.0 ± 10.1% vs. 39.1 ± 18.2%, p = 0.008, respectively). The expression of P-selectin also tended to be lower in preterm neonates, with significant positive correlations between P-selectin expression and PNA formation. Conclusions: The potential formation of PNAs correlates with gestational age. This suggests that the development of functional competencies of platelets and neutrophils continues throughout gestation, progressively enabling interactions between them.

show abstract

Section: Discussionsupporting

confidence: 91%

Platelet-Neutrophil Interactions Are Lower in Cord Blood of Premature Newborns

et al. 2018

View full text Add to dashboard Cite

show abstract

“…For platelet-expressed adhesion receptors, Schlagenhauf et al reported higher GPIba levels in term newborn platelet lysates isolated from human cord blood. 12 Earlier studies, including data from the same group, found similar receptor numbers for GPIb on platelets in human neonates and adults. 13,14 More recently, surface levels of GPIba and GPIbb were measured by flow cytometry in mouse samples of different gestational ages, revealing reduced levels of GPIb in fetal platelets compared with adults.…”

Section: Surface Expression Of Adhesion Relevant Moleculesmentioning

confidence: 91%

“…17 Similarly, the investigators of that study reported reduced levels of GPIIb/IIIa in neonatal vs adult platelets, whereas other investigators described equal amounts of GPIIb/IIIa in term newborns compared with adults. 12,15,17 We investigated GPIIb/IIIa expression in mouse fetuses and found an overall increase in GPIIb expression at embryonic day (E)13.5 (of 21 days' gestation) compared with later time points. 3…”

Section: Surface Expression Of Adhesion Relevant Moleculesmentioning

confidence: 99%

Ontogeny of platelet function

Margraf

Nußbaum

Sperandio³

2019

Blood Advances

View full text Add to dashboard Cite

Although the hemostatic potential of adult platelets has been investigated extensively, regulation of platelet function during fetal life is less clear. Recent studies have provided increasing evidence for a developmental control of platelet function during fetal ontogeny. Fetal platelets feature distinct differences in reactive properties compared with adults. These differences very likely reflect a modified hemostatic and homeostatic environment in which platelet hyporeactivity contributes to prevent pathological clot formation on the one hand but still ensures sufficient hemostasis on the other hand. In this review, recent findings on the ontogeny of platelet function and reactivity are summarized, and implications for clinical practice are critically discussed. This includes current platelet-transfusion practice and its potential risk in premature infants and neonates.

show abstract

“…12 Recently, decreased expression of PAR-1 and PAR-4 receptors was described in neonatal platelets, thus explaining the decreased response to thrombin. 16,17 While this platelet hyporeactivity would have been expected to result in a bleeding tendency, Andrew et al . in 1999 reported that full term neonates had shorter bleeding times (BTs) than healthy adults.…”

Section: Primary Hemostasis In the Neonatementioning

confidence: 99%