Comparative Evaluation of Non-Radioactive in situ Hybridization Techniques for Pathologic Diagnosis of Viral Infection

Seyda, Midas; Scheele, Thomas; Neumann, Rainer; Krueger, G. R. F.

doi:10.1016/s0344-0338(88)80186-9

Cited by 11 publications

(1 citation statement)

References 43 publications

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“…SupT1 cells showed characteristic cytopathic giant cell formation upon HHV-7 infection. Infection was also demonstrated by indirect immunofluorescence assays using anti-HHV-7 positive patient sera reacting with HHV-7 infected cells but not with cells infected by HHV-6, CMV, EBV, or HSV; immunohistology (APAAP) using monoclonal antibodies against HHV-7 gp 110/56 viral envelope proteins (H-AR-7, kindly provided by J. Luka, Eastern Virginia Medical College, USA); and in situ hybridization with the pVL17A.1 probe of Berneman and the polymerase chain amplification reaction according to Klotman and his group [3,4,24,25,46] (Fig. 1) which was also effective.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural study of human herpesvirus-7 replication in tissue culture

et al. 1997

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Human herpesvirus 7 (HHV-7) was grown in a CD4+ lymphoblastic cell line (SupT1) and in cord blood mononuclear cells (CBMC). Virus infection was demonstrated by immunohistology with positive control sera, with monoclonal antibodies and by in situ hybridization for viral DNA. Cytopathic effects following HHV-7 infection generally resemble those after HHV-6 infection but are less pronounced. The ultrastructural appearance of HHV-7 and the replicative stages were similar to those described by Kramarsky and Sander for HHV-6. There were some minor discrepancies, including quite an extensive and space-filling tegument, a slightly different structure of the nucleoid, the frequent finding of nucleocapsids without any visible core and apparently scarce or delicate spikes on the envelope. These differences may suggest HHV-7 rather than HHV-6, but this finding needs confirmation. Mature HHV-7 particles measured 170 nm in diameter, with nucleocapsids of 90-95 nm and a tegument of about 30 nm.

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Section: Methodsmentioning

confidence: 99%