Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation

Sandhya, Chandran; Sumantha, Alagarsamy; Szakács, George; Pandey, Ashok

doi:10.1016/j.procbio.2004.12.001

Cited by 303 publications

(198 citation statements)

References 34 publications

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“…One variable was varied at a time keeping other variable constant, it included the incubation time (24-120 h), incubation temperature (20-45°C), pH (4)(5)(6)(7)(8)(9)(10)(11)(12), salt concentration (2-6% NaCl), effect of nitrogen sources such as peptone, yeast extract, urea, ammonium chloride, ammonium sulphate, casein and various carbon sources such as arabinose, fructose, D-glucose, mannose, starch, xylose. For each parameter optimization, three sets of independent experiments were carried out and the average values were reported.…”

Section: Estimation Of Exo-chitinase Activitymentioning

confidence: 99%

“…can restrict the growth of soil-borne pathogens through hydrolysis of cell wall associated chitin [3]. Chitinase production by T. harzianum was found to be induced using wheat bran-based solid medium containing 1% colloidal chitin and the chitinase lead to the lysis of the phytopathogenic fungus Colletotrichum gloeosporioides [4]. In this context, solid state fermentation (SSF) has emerged as a promising technology for the enzyme production from cheaply available agro-industrial residues such as wheat bran, rice bran, oil cakes, etc.…”

Section: Introductionmentioning

confidence: 99%

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Studies on Exo-Chitinase Production from Trichoderma asperellum UTP-16 and Its Characterization

et al. 2011

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The growth conditions for chitinase production by Trichoderma asperellum UTP-16 in solid state fermentation was optimized using response surface methodology based on central composite design. The chitinase production was optimized, using one-factor at a time approach, with six independent variables (temperature, pH, NaCl, incubation period, nitrogen and carbon sources) and 3.31 Units per gram dry substrate (U gds -1 ) exo-chitinase yield was obtained. A 21.15% increase was recorded in chitinase activity (4.01 U gds -1 ) through surface response methodology, indicates that it is a powerful and rapid tool for optimization of physical and nutritional variables. Further, efficiency of crude enzyme was evaluated against phytopathogenic Fusarium spp. and a mycelial growth inhibition up to 3.5-6.5 mm was achieved in well diffusion assay. These results could be supplemented as basic information for the development of enzyme based formulation of T. asperellum UTP-16 and its use as a biocontrol agent.

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Section: Estimation Of Exo-chitinase Activitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Studies on Exo-Chitinase Production from Trichoderma asperellum UTP-16 and Its Characterization

et al. 2011

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“…Also due to different diffusion, stimulation by regulator molecules (inducers) is better in SSF and the product degradation is lower (Oojikaas et al 2000, Aguilar et al 2004, Viniegra-Gonzalez & Favela-Torres 2006. For all these and other reasons, productivity and enzyme yields in SSF can be higher than in SmF (Díaz-Godínez et al 2001, Viniegra-González et al 2003, Matsumoto et al 2004, Sandhya et al 2005, Shah & Madamwar 2005, Hongzhang et al 2006, Patil & Dayanand 2006. Disadvantageous in SSF is, however, the lower thermal conductivity of the air space compared to the liquid enclosing micro-organisms in SmF.…”

Section: Solid State Fermentation (Ssf)mentioning

confidence: 99%

Wood production, wood technology, and biotechnological impacts

Kües¹

2007

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“…The extracellular production of fungi collagenases makes particularly easy its recovery after the end of fermentation [19]. Studies have reported the biosynthesis of collagenases by fungi belonging to the different genera, such as Aspergillus, Cladosporium, Alternaria, Penicillium [8,[20][21][22], Candida [23], Microsporum [24] and Rhizoctonia [14].…”

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Collagenase by Penicillium sp. UCP 1286 Isolated From Caatinga Soil

2016

J App Biol Biotech

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A new Penicillium sp. strain isolated from the soil of Caatinga, a Brazilian Biome (UCP 1286) was selected for collagenase production. Fermentation system allowing obtention of collagenolytic activity about 2.7 times higher than existing data, with the highest values of collagenolytic and specific activity (379.80 U/mL, 1460.77 U/mg, respectively), after 126 hours. Applying a factorial design, enzyme production was increased by about 65% compared to the preliminary results. The factorial design demonstrated the existence of two factors with statistical significance on the production of the enzyme: pH and temperature, both with negative effects. Enzyme was found to be more active at pH 9.0 and 37 °C, and also to be very stable in comparison with the collagenase produced by other microorganisms. The enzyme seems to belong to collagenolytic serine proteases family. Concerning the substrate specificity, it was observed that the highest enzyme activity corresponds to azocoll, there was no relevant activity on azocasein and the enzyme showed to be more specific to type V collagen and gelatin than the commercial colagenase produced by Clostridium histolyticum. Major band observed at electrophoresis was approximately 37 kDa. Zymogram analysis confirmed the collagenolytic activity. All data indicates this enzyme as promising biotechnology product.

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Comparative evaluation of neutral protease production by Aspergillus oryzae in submerged and solid-state fermentation

Cited by 303 publications

References 34 publications

Studies on Exo-Chitinase Production from Trichoderma asperellum UTP-16 and Its Characterization

Studies on Exo-Chitinase Production from Trichoderma asperellum UTP-16 and Its Characterization

Wood production, wood technology, and biotechnological impacts

Production and Characterization of Collagenase by Penicillium sp. UCP 1286 Isolated From Caatinga Soil

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