Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen

Nabi, Mohammad Mahdi; Kohram, Hamid; Zhandi, Mahdi; Mehrabani-Yeganeh, Hassan; Sharideh, Hossein; Zare-Shahaneh, Ahmad; Esmaili, Vahid

doi:10.1016/j.cryobiol.2015.11.005

Cited by 25 publications

(11 citation statements)

References 32 publications

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“…Semen from each rooster was diluted with modified Beltsville Poultry Semen Extender and six hens were inseminated with a dose of 200 mL per hen (100 Â 10 6 spermatozoa per dose; Ali et al 2017). As per Nabi et al (2016), with minor modification, the diluting extender was composed of potassium phosphate dibasic trihydrate (33.25 mM), sodium L-glutamate (51.26 mM), D-fructose (27.75 mM), sodium acetate trihydrate (23.51 mM), N-tris(hydroxymethyl)methyl-2aminoethanesulfonic (13.95 mM), potassium citrate tribasic monohydrate (1.85 mM), potassium phosphate monobasic (5.14 mM) and magnesium chloride anhydrous (3.15 mM) with an osmotic pressure (Osmomat 030-D; Gonotec) and pH (3520; Jenway) of 310 mOsmol kg À1 and 7.4 respectively.…”

Section: Artificial Inseminationmentioning

confidence: 99%

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology

Ansari

Zhandi

Kohram

et al. 2018

Reprod. Fertil. Dev.

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Male broiler breeders (n = 32) of 55 weeks of age were administered four different doses of capsulated d-aspartate (DA; 0, 100, 200 or 300 mg kg−1 day−1, p.o. (DA0, DA100, DA200 and DA300 respectively)) for 12 successive weeks to assess reproductive performance, blood testosterone, testicular histology and transcript levels of steroidogenic acute regulatory protein (StAR), cholesterol side-chain cleavage enzyme (P450scc), androgen receptor (AR), LH receptor (LHR), 3β-hydroxysteroid dehydrogenase (3BHSD), proliferating cell nuclear antigen (PCNA), glutamate ionotropic receptor NMDA type subunit 1 (GRIN1) and glutamate ionotropic receptor NMDA type subunit 2B (GRIN2B). Blood samples and ejaculates were collected, and bodyweight was recorded weekly for 10 weeks. AI was performed weekly for the last 2 weeks to determine the number of sperm penetration holes in the perivitelline layer, fertility and hatchability. Testes histology and transcript levels were evaluated in the 12th week. Bodyweight, numbers of Leydig cells and blood vessels, testis index and levels of sperm abnormalities were not affected (P > 0.05) by the treatment. However, sperm total and forward motility, plasma membrane integrity and functionality of sperm, ejaculate volume, testosterone concentration and fertility were higher (P < 0.05) in both the DA200 and DA300 groups compared with the other groups. In the DA100 and DA200 groups, sperm concentration, number of spermatogonia, thickness of the seminiferous epithelium and the diameter of tubules were significantly higher (P < 0.05) than the other DA-treated groups. The number of penetration holes, hatchability and malondialdehyde concentration were higher in the DA200, all DA-treated and DA300 groups respectively compared with the control and other treatment groups. Except for P450scc, AR, LHR and PCNA transcript levels in the DA300 groups, the relative expression of the genes evaluated improved significantly in the other DA-treated groups. Based on these experimental findings, it is concluded that DA improves reproductive performance of aged roosters.

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Section: Artificial Inseminationmentioning

confidence: 99%

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology

Ansari

Zhandi

Kohram

et al. 2018

Reprod. Fertil. Dev.

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“…The motilities of fresh and frozen–thawed semen from each bird were analyzed using a computer-assisted sperm analyzer system (WLJX-9000 Weili Color Sperm Analysis System, Weili New Century Science & Tech Dev., Beijing, China) and with settings adjusted to detecting avian sperm ( Davila et al., 2015 , Nabi et al., 2016 ). Five randomly selected microscopic fields were analyzed for each semen sample, and the mean value represented the sperm motility of samples.…”

Section: Methodsmentioning

confidence: 99%

Comparative transcriptome analysis digs out genes related to antifreeze between fresh and frozen–thawed rooster sperm

Xing

Huang

et al. 2020

Poultry Science

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The objective of this study was to investigate differences in mRNA expression between fresh and frozen–thawed sperm in roosters. In trial 1, gene expression profiles were measured using microarray with Affymetrix GeneChip Chicken Genome Arrays. The results showed that 2,115 genes were differentially expressed between the 2 groups. Among these genes, 2,086 were significantly downregulated and 29 were significantly upregulated in the frozen–thawed sperm group. Gene Ontology ( GO ) analysis showed that more than 1,000 differentially expressed genes ( DEG ) of all significantly regulated genes were involved in GO terms including biological processes, molecular function, and cellular component. Kyoto Encyclopedia of Genes and Genomes analysis showed that DEG were significantly ( P < 0.05) enriched on ribosome, oxidative phosphorylation, proteasome, cell cycle, oocyte meiosis, and spliceosome pathways. In trial 2, ejaculated semen was collected from 18 roosters and divided into 5 recombinant HSP90 protein–supplemented groups (0.01, 0.1, 0.5, 1, or 2 μg/mL) and one control group with no recombinant HSP90 protein supplementation to evaluate the effect of recombinant HSP90 protein in the extender on post-thaw quality of rooster semen. The results showed that post-thaw sperm viability and motility was significantly improved ( P < 0.05) in the extender containing 0.5 and 1 μg/mL of recombinant HSP90 protein compared with the control. Our preliminary results will provide a valuable basis for understanding the potential molecular mechanisms of cryodamage in frozen–thawed sperm and theoretical guidance to improve the fertility of frozen–thawed chicken sperm.

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“…Collected semen from each rooster was individually extended by extender containing potassium phosphate dibasic trihydrate (33.25 mM), sodium l‐glutamate (51.26 mM), D‐fructose (27.75 mM), sodium acetate trihydrate (23.51 mM), N‐tris (hydroxymethyl) methyl‐2‐aminoethanesulfonic (13.95 mM), potassium citrate tribasic monohydrate (1.85 mM), potassium phosphate monobasic (5.14 mM), magnesium chloride anhydrous (3.15 mM), soya bean lecithin (0.5% w/v) and dimethyl sulphoxide (DMSO) (4%, v/v). Before DMSO and soya bean lecithin inclusion, osmotic pressure (Osmomat 030‐D Gonotec Germany) and pH (3520 Jenway, United Kingdom) of the extender were adjusted to 310 mOsmol/kg and 7.4, respectively (Nabi et al., ). Extended samples containing 400 × 10 6 spermatozoa/ml were loaded into 0.25‐ml straws, sealed and cooled to 5°C for an hour.…”

Section: Methodsmentioning

confidence: 99%

Orally administered Chrysin improves post‐thawed sperm quality and fertility of rooster

Zhandi

Ansari

Roknabadi

et al. 2017

Reprod Domestic Animals

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Chrysin is a bioflavonoid compound found in passion flower, chamomile, propolis and honey at high levels. Post-thawed sperm quality and fertility of Chrysin-fed roosters were assessed in this study. Twenty 40-week-old male broiler breeders were randomly divided into four groups and fed basal diet supplemented with different levels of Chrysin including 0 (Ch-0), 25 (Ch-25), 50 (Ch-50) or 75 (Ch-75) mg/day for 12 consecutive weeks. Semen samples were weekly collected from 6th to 9th week of experiment to evaluate some sperm quality parameters including total and progressive motility, plasma membrane integrity and functionality (in fresh and post-thawed samples) and mitochondrial activity (only in post-thawed samples). Also, collected semen samples from 10th, 11th and 12th week of experiment were frozen and then artificially inseminated to test fertility rate. According to the results, an improvement in both fresh and post-thawed sperm quality including total [fresh: 88.00 ± 0.58 and 87.25 ± 0.67 (p < .01); post-thawed: 51.07 ± 2.05 and 52.72 ± 1.96 (p < .01)] and progressive motility [fresh: 76.00 ± 0.58 and 78.25 ± 0.65 (p < .01); post-thawed: 40.61 ± 2.01 and 39.88 ± 2.01 (p < .01)], plasma membrane integrity [fresh: 91.60 ± 0.58 and 89.85 ± 0.59 (p < .01); post-thawed: 56.99 ± 1.86 and 54.39 ± 1.86 (p < .01)] and functionality [fresh: 75.40 ± 0.42 and 77.90 ± 0.96 (p < .01); post-thawed: 45.69 ± 1.71 and 46.35 ± 1.71 (p < .01)] was noted for both Ch-50 and Ch-75, respectively, groups compared to control group. Despite no significant change in mitochondrial activity, fertility rate of post-thawed spermatozoa was significantly improved in all Chrysin-fed groups compared to Ch-0 group. In conclusion, oral Chrysin administration to roosters could ameliorate cryopreservation-induced impairment of sperm quality and fertility rate.

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Comparative evaluation of Nabi and Beltsville extenders for cryopreservation of rooster semen

Cited by 25 publications

References 32 publications

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology

D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology

Comparative transcriptome analysis digs out genes related to antifreeze between fresh and frozen–thawed rooster sperm

Orally administered Chrysin improves post‐thawed sperm quality and fertility of rooster

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