Comparative evaluation of miniaturized and conventional NIR spectrophotometer for estimation of fatty acids in cheeses

Assessment of bovine milk fatty acids using miniaturised near infrared spectrophotometer

“…Similarly, Soulat et al (2020) reported high correlation among FA but variable predictions performances among FA (Soulat et al 2020). Independent studies observed that despite correlation between fat and fatty acid concentration, the importance of spectral features varies depending on fatty acid, indicating the ability of NIRS to differentiate among the FA (Muncan et al 2021;Reis et al 2022). It can be argued that NIRS is not capable of discrimination of the saturated FA C4:0 and C6:0 since the difference between C4:0 and C6:0 is due two -(CH2)-unities.…”

“…spectral to noise) compared with other instruments has been presented in a previous study (Reis et al . 2022). Compared to raw spectra (Figure 1a), SVN reduced variation (as measured by standard deviation, shaded areas, Figure 1b).…”

“…Milk samples were equilibrated to room temperature (20°C) and gently agitated before analysis to prevent fat separation. Samples were aliquoted into 1 mL plastic cuvettes and scanned using the NIR device (DLPâ NIRscan TM Nano, Texas Instruments) utilising settings identified previously (Reis et al 2022). A Spectralon tile was scanned after the spectrometer had been equilibrated at room temperature for 1 h and before scanning the samples.…”

“…These Savytzky-Golay parameters have been able to reduce noise variation without overfitting spectra (with addition of bias) in our previous studies. SNV has also successfully reduced the effect of unwanted scattering (Reis et al 2022).…”

“…Miniaturised instruments using NIR are userfriendly and cost-effective (Reis et al 2022). These spectrometers have been used in milk assessment to discriminate samples based on fat content (R 2 :0.98, RMSE: 0.002 g/mL) and composition (Risoluti et al 2020;Riu et al 2020), but the prediction performance of the concentration of individual FA has not been reported.…”