Comparative evaluation of hydroxyapatite and nano-bioglass in two forms of conventional micro- and nano-particles in repairing bone defects (an animal study)

Nosouhian, S; Razavi, Mohammad; Jafari-Pozve, Nasim; Rismanchian, Mansour

doi:10.4103/0970-9290.167645

Cited by 9 publications

(4 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As reported in the study HA et al (2015), nanoHA-induced cellular regulation in osteoblast did not occur in cells cultured with nanosized silica [ 138 ]. This is also in line with in vivo findings; after 15 days of implantation, nanoHA was superior to nano-bioglass, causing the highest bone formation rate [ 139 ]. Thus, we indicated that the cellular response induced by nanoHA was due to the material property of nanoHA.…”

Section: Other Cellular Events Induced By Hasupporting

confidence: 88%

Signaling Pathway and Transcriptional Regulation in Osteoblasts during Bone Healing: Direct Involvement of Hydroxyapatite as a Biomaterial

et al. 2021

View full text Add to dashboard Cite

Bone defects and periodontal disease are pathological conditions that may become neglected diseases if not treated properly. Hydroxyapatite (HA), along with tricalcium phosphate and bioglass ceramic, is a biomaterial widely applied to orthopedic and dental uses. The in vivo performance of HA is determined by the interaction between HA particles with bone cells, particularly the bone mineralizing cells osteoblasts. It has been reported that HA-induced osteoblastic differentiation by increasing the expression of osteogenic transcription factors. However, the pathway involved and the events that occur in the cell membrane have not been well understood and remain controversial. Advances in gene editing and the discovery of pharmacologic inhibitors assist researchers to better understand osteoblastic differentiation. This review summarizes the involvement of extracellular signal-regulated kinase (ERK), p38, Wnt, and bone morphogenetic protein 2 (BMP2) in osteoblastic cellular regulation induced by HA. These advances enhance the current understanding of the molecular mechanism of HA as a biomaterial. Moreover, they provide a better strategy for the design of HA to be utilized in bone engineering.

show abstract

Section: Other Cellular Events Induced By Hasupporting

confidence: 88%

Signaling Pathway and Transcriptional Regulation in Osteoblasts during Bone Healing: Direct Involvement of Hydroxyapatite as a Biomaterial

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Biomaterials fabricated into nanoscale (1-100 nm) structures (nanomaterials) have been shown to mimic the native ECM and promote cell adhesion and osteogenic differentiation. [20][21][22] Graphene-based nanomaterials have recently been recognized as useful components of bone tissue engineering scaffolds. Graphene derivatives are preferred over the pristine form and can be produced relatively easily by functionalization of pristine graphene, with the ultimate goal of reducing pristine graphene's toxicity and increasing its usability in biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

<p>Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis</p>

Newby¹,

Masi²,

Griffin³

et al. 2020

IJN

View full text Add to dashboard Cite

Purpose: The extracellular matrix (ECM) labyrinthine network secreted by mesenchymal stem cells (MSCs) provides a microenvironment that enhances cell adherence, proliferation, viability, and differentiation. The potential of graphene-based nanomaterials to mimic a tissue-specific ECM has been recognized in designing bone tissue engineering scaffolds. In this study, we investigated the expression of specific ECM proteins when human fat-derived adult MSCs adhered and underwent osteogenic differentiation in the presence of functionalized graphene nanoparticles. Methods: Graphene nanoparticles with 6-10% oxygen content were prepared and characterized by XPS, FTIR, AFM and Raman spectroscopy. Calcein-am and crystal violet staining were performed to evaluate viability and proliferation of human fat-derived MSCs on graphene nanoparticles. Alizarin red staining and quantitation were used to determine the effect of graphene nanoparticles on osteogenic differentiation. Finally, immunofluorescence assays were used to investigate the expression of ECM proteins during cell adhesion and osteogenic differentiation. Results: Our data show that in the presence of graphene, MSCs express specific integrin heterodimers and exhibit a distinct pattern of the corresponding bone-specific ECM proteins, primarily fibronectin, collagen I and vitronectin. Furthermore, MSCs undergo osteogenic differentiation spontaneously without any chemical induction, suggesting that the physicochemical properties of graphene nanoparticles might trigger the expression of bone-specific ECM. Conclusion: Understanding the cell-graphene interactions resulting in an osteogenic niche for MSCs will significantly improve the application of graphene nanoparticles in bone repair and regeneration.

show abstract

“…Both support the attachment and proliferation of bone‐forming osteoblast cells and assist the formation of mineralized bone matrix 23,24 and have been used in various FDA‐approved medical devices since 1985 25,26 . Several studies suggest BG superiority over HA because of the following reasons 27–29 : (1) The faster proliferation of osteoblasts and bone formation; (2) Better adhesion and bonding to surrounding soft tissues elements e.g., collagen without induction of fibrous capsule in vivo; (3) Higher bioactivity due to ion release and rich silica layer; (4) Anti‐microbial properties; (5) Finally bioglass' degradation products could induce the growth factors production and cell proliferation. They could also trigger the gene expression of osteoblasts 30 …”

Section: Introductionmentioning

confidence: 99%

“…21,22 Both support the attachment and proliferation of bone-forming osteoblast cells and assist the formation of mineralized bone matrix 23,24 and have been used in various FDA-approved medical devices since 1985. 25,26 Several studies suggest BG superiority over HA because of the following reasons [27][28][29] : (1) The faster proliferation of osteoblasts and bone formation; (2) Better adhesion and bonding to surrounding soft tissues elements e.g., collagen without induction of fibrous capsule in vivo;…”

mentioning

confidence: 99%

Injectable cell‐laden hybrid bioactive scaffold containing bioactive glass microspheres

Rahimnejad

Charbonneau

et al. 2023

J Biomedical Materials Res

View full text Add to dashboard Cite

The rising incidence of bone disorders has resulted in the need for minimally invasive therapies to meet this demand. Injectable bioactive filler, alone or with cells, could be applied in a minimally invasive manner to fulfill irregular cavities in non‐load bearing sites, which do not require high mechanical properties. Thermosensitive chitosan hydrogels that transition from a liquid to a mechanically stable solid at body temperature provide interesting features as in‐situ injectable cytocompatible biomaterials, but they are not osteoconductive. Osteoconductivity can be applied in combination with bioactive ceramics e.g., 45S5‐Bioglass® (BG). However, BG addition in chitosan hydrogels results in pH elevation, due to rapid ions release, which adversely affects gel formation, mechanical properties, and cytocompatibility. To address this, we created hybrid hydrogels, where BG is concentrated in chitosan‐based microbeads, incorporated in in‐situ gelling chitosan hydrogels. We then compared the hybrid hydrogels' properties to chitosan hydrogels with homogenously distributed BG. By varying the stirred emulsification process, BG percentage, and CH formulation, we could tune the microbeads' properties. Incorporation of BG microbeads drastically improved the hydrogel's compressive modulus in comparison to homogeneously distributed BG. It also strongly increased the survival and metabolic activities of encapsulated cells. Calcium/phosphate increase on BG microbeads suggests hydroxyapatite formation. The small diameter of microbeads allows minimally invasive injection through small needles. The feasibility of freezing and thawing microbeads provides the possibility of long‐term storage for potential clinical applications. These data indicate that this hybrid hydrogel forms a promising injectable cell‐laden bioactive biomaterial for the treatment of unloaded bone defects.

show abstract

Comparative evaluation of hydroxyapatite and nano-bioglass in two forms of conventional micro- and nano-particles in repairing bone defects (an animal study)

Abstract: Nano-HA and nano-bioglass biomaterials showed promising results when compared to conventional micro-particles in the repair of bone defects.

Cited by 9 publications

References 34 publications

Signaling Pathway and Transcriptional Regulation in Osteoblasts during Bone Healing: Direct Involvement of Hydroxyapatite as a Biomaterial

Signaling Pathway and Transcriptional Regulation in Osteoblasts during Bone Healing: Direct Involvement of Hydroxyapatite as a Biomaterial

<p>Functionalized Graphene Nanoparticles Induce Human Mesenchymal Stem Cells to Express Distinct Extracellular Matrix Proteins Mediating Osteogenesis</p>

Injectable cell‐laden hybrid bioactive scaffold containing bioactive glass microspheres

Contact Info

Product

Resources

About