Comparative evaluation of eight commercial enzyme linked immunosorbent assays and 14 simple assays for detection of antibodies to HIV

Beelaert, Greet; Vercauteren, G.; Fransen, Katrien; Mangelschots, Marianne; Rooy, Marie; Garcia-Ribas, S.; Groen, G van der

doi:10.1016/s0166-0934(02)00102-7

Cited by 30 publications

(22 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lateral flow technology has been introduced and validated in veterinary, feed and food, and human sample analysis (eg, HIV, 16,17 influenza, 18 or respiratory syncytial virus 19 tests). Recently it has found its application for rapid detection of mite allergens in the homes, 1,20 and was shown to be very sensitive and well correlated with results of the reference EIA methods.…”

Section: Discussionmentioning

confidence: 99%

“…A similar observation was made for duplicate airborne stationary PAS-6 samples. All wipe samples from control areas in the bakery and 1 field blank (wipe) were positive for amylase allergen, but at relatively (1, 2), packing area (3,4), and bakery office (5); airborne stationary PAS-6 (7,12,13,16) and S&S samples (18,19); airborne personal PAS-6 samples (8-10, 14, 15); flour samples (20-24); field blanks (6,11,17); and standard preparation of amylase allergen at 40 ng/mL (25). Strongly positive (11) were samples 1 and 2 (wipes, dough-making area); 10 (personal airborne dust, manager); 20, 22, 23, 24 (flours); and 25 (standard).…”

Section: Allergen Quantification By Eiamentioning

confidence: 99%

See 1 more Smart Citation

Rapid detection of fungal α-amylase in the work environment with a lateral flow immunoassay

Bogdanović

Koets

Sander

et al. 2006

Journal of Allergy and Clinical Immunology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Allergen Quantification By Eiamentioning

confidence: 99%

Rapid detection of fungal α-amylase in the work environment with a lateral flow immunoassay

Bogdanović

Koets

Sander

et al. 2006

Journal of Allergy and Clinical Immunology

View full text Add to dashboard Cite

“…These tests are enzyme-linked immunosorbent assays where magnetic beads are used as solid supports. Sensitivities and specificities of 98% or more have been reported for both tests (3,36). All sera were analyzed in the laboratory of Bionor.…”

Section: Serum Samplesmentioning

confidence: 99%

Prevalence of Antibodies against Herpes Simplex Virus Types 1 and 2 in Children and Young People in an Urban Region in Tanzania

Kasubi

Nilsen

Marsden³

et al. 2006

J Clin Microbiol

View full text Add to dashboard Cite

Herpes simplex virus type 1 (HSV-1) is transmitted by close contact, both sexual and nonsexual, and infections are acquired during childhood and adolescence. Herpes simplex virus type 2 (HSV-2), however, is thought to be transmitted mainly by sexual contact. Most HSV-2 infections are consequently expected to occur after the onset of sexual activity. Recent reports indicate an increasing prevalence of HSV-2 on the African continent, but most studies have been performed on adult cohorts. In the present study, we collected sera from Tanzanian children and young persons from 1 to 20 years old, with at least 100 individuals in each age group. Antibodies against HSV-1 and HSV-2 were detected by an in-house Western blot method which was shown to perform well in comparison with a commercial Western blot assay. Type-specific antibodies were also analyzed by two noncommercial enzyme-linked immunosorbent assay methods based upon the antigenicities of branched synthetic oligopeptides corresponding to epitopes in glycoprotein G of HSV-1 or HSV-2. The prevalence of HSV-1 antibodies increased gradually from 73% for the age group of 1 to 4 years to 92% for the age group of 17 to 20 years. The prevalence of HSV-2 antibodies was unexpectedly high, as 15% of the children were infected by the age of 8 years, with the incidence increasing gradually to 40% in the age group of 17 to 20 years. The reason for this unexpectedly high frequency is not clear but could suggest that nonsexual transmission of HSV-2 is more common than previously thought. There was no statistically significant association between seropositivities for HSV-2 and human immunodeficiency virus.

show abstract

“…Therefore, 100% clinical specificity was obtained for both polypeptides. The 100% specificity of the ELISA-HIV2 assay compares favorably to the specificity of the two licensed HIV-2 serodiagnosis assays (Ͻ92%) (2, 7) and to the specificity of most mixed HIV-1/HIV-2 assays (mean, 99%; range, 94.6% to 100% for assays based on recombinant proteins; mean, 98%; range 90.4% to 100% for assays based on synthetic peptides) (1,4,5,12,14,22,28,31,38,42,43,45,48).…”

mentioning

confidence: 95%

“…The gp36 ectodomain is highly conserved and elicits a type-specific antibody response (13,33). Hence, most licensed diagnostic assays incorporate gp36-derived antigens to detect HIV-2-specific antibodies (1,4,12,28,29,38,42,45,48). The sensitivity of these assays to detect HIV-2 seroconversions has not been formally tested.…”

mentioning

confidence: 99%

Use of a New Dual-Antigen Enzyme-Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins

et al. 2006

View full text Add to dashboard Cite

A dual-antigen enzyme-linked immunosorbent assay specific for human immunodeficiency virus type 2 (HIV-2) envelope proteins, ELISA-HIV2, was developed with two new recombinant polypeptides, rpC2-C3 and rgp36, derived from the HIV-2 envelope. The diagnostic performance was determined with HIV-2, HIV-1, and HIV-1/2 samples. Both polypeptides showed 100% specificity. Clinical sensitivity was 100% for rgp36 and 93.4% for rpC2-C3. ELISA-HIV2 may be used for the specific diagnosis and confirmation of HIV-2 infection.

show abstract

Comparative evaluation of eight commercial enzyme linked immunosorbent assays and 14 simple assays for detection of antibodies to HIV

Cited by 30 publications

References 8 publications

Rapid detection of fungal α-amylase in the work environment with a lateral flow immunoassay

Rapid detection of fungal α-amylase in the work environment with a lateral flow immunoassay

Prevalence of Antibodies against Herpes Simplex Virus Types 1 and 2 in Children and Young People in an Urban Region in Tanzania

Use of a New Dual-Antigen Enzyme-Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins

Contact Info

Product

Resources

About