Comparative evaluation of efficacy of Zinc oxide and Copper oxide nanoparticles as antimicrobial additives in alginate impression materials

Ginjupalli, Kishore; Alla, Rama Krishna; Shaw, Tushar; Tellapragada, Chaitanya; Gupta, Lokendra; Upadhya, P. Nagaraja

doi:10.1016/j.matpr.2018.05.117

Cited by 14 publications

(8 citation statements)

References 27 publications

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“…Recently, copper oxide nanoparticles (CuO NPs) have been extensively used in different areas of science and technology due to their good thermal, electrical conductivity, and low cost [20]. Previous studies have demonstrated that the incorporation of NPs in orthodontic brackets and alginates significantly reduces the amount of microbial biofilm [21,22]. Recently, in order to reduce both the cost and the toxicity of CuO NPs, biological sources such as plants have been used instead of chemical methods, which have shown an additional antibacterial activity, as well [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial and Antibiofilm Activities of Copper Oxide Nanoparticles within Soft Denture Liners against Oral Pathogens

Ansarifard

Zareshahrabadi

Sarafraz

et al. 2021

Bioinorganic Chemistry and Applications

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Objectives. Soft denture liners provide a favorable environment for adhesion and colonization of microorganisms. This in vitro study aimed to examine the efficacy of different concentrations of copper oxide nanoparticles (CuO NPs) incorporation into soft denture liner on the biofilm formation of the microbial species. Methods. Field Emission Scanning Electron Microscopy (FESEM) images from NPs were recorded. Antifungal susceptibility testing of CuO NPs against five standard strains of Candida albicans (CBS 10261, 1905, 1912, 1949, 2730), Streptococcus mutans (ATCC35668), Streptococcus sobrinus (ATCC27607), and Streptococcus salivarius (ATCC9222) was performed by the broth microdilution method with the Clinical and Laboratory Standards Institute reference method. The biofilm inhibition percentages of CuO NPs on the soft denture liners were determined by XTT assay. Results. The characterization of CuO NPs by scanning electron microscope (SEM) analyses confirmed the synthesis of NPs with appropriate structure and size with a mean diameter of 18.3 ± 9.1 nm. The CuO NPs successfully inhibited the growth of the tested standard strains of C. albicans and Streptococcus spp. at concentrations ranging from 64 to 128 µg mL−1. Indeed, incorporation of CuO NPs at a concentration of 500 µg mL−1 into the soft denture liners exhibited a significant activity (75%) in inhibition of C. albicans. biofilm formation in a dose-dependent manner. The biofilm formation of C. albicans in the presence of CuO NPs was lower than Streptococcus spp. in comparison with the control group ( p < 0.05 ). Conclusion. Incorporation of CuO NPs significantly decreased the colonization and plaque formation of the oral pathogens, especially C. albicans accumulation. These NPs may be useful as a promising agent for the antimicrobial management of soft denture liner materials.

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Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial and Antibiofilm Activities of Copper Oxide Nanoparticles within Soft Denture Liners against Oral Pathogens

Ansarifard

Zareshahrabadi

Sarafraz

et al. 2021

Bioinorganic Chemistry and Applications

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“…The issue could be partially overcome by using simple polymeric systems with inherent antimicrobial properties without needing actual drugs to be incorporated. Several new conceptions have been presented recently [ 3 , 4 , 5 ]. One of these approaches could be the use of alginate particles cross-linked with bivalent metal ions [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration

et al. 2021

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Antimicrobial agent abuse poses a serious threat for future pharmacotherapy, including vaginal administration. The solution can be found in simple polymeric systems with inherent antimicrobial properties without the need to incorporate drugs, for instance alginate beads cross-linked by bivalent ions. The main goal of the presented study was to provide improvement on the well-documented cytotoxicity of Cu2+ cross-linked alginate. Alginate beads were prepared by external ionotropic gelation by cross-linking with Cu2+, Ca2+ and Zn2+ ions, separately and in mixtures. Morphological properties, swelling capacity, ion release and efficacy against the most common vaginal pathogens (C. albicans, E. coli, E. faecalis and virus strain—human herpesvirus type 1) were evaluated. The prepared particles (particle size 1455.68 ± 18.71–1756.31 ± 16.58 µm) had very good sphericity (0.86 ± 0.04–0.97 ± 0.06). In mixture samples, Cu2+ hampered second ion loading, and was also released incompletely (18.75–44.8%) compared to the single ion Cu2+ sample (71.4%). Efficacy against the selected pathogens was confirmed in almost all samples. Although anticipating otherwise, ion mixture samples did not show betterment over a Cu2+ cross-linked sample in cytotoxicity–pathogen efficacy relation. However, the desired improvement was found in a single ion Zn2+ sample whose minimal inhibition concentrations against the pathogens (0.6–6.12 mM) were close to, or in the same mathematical order as, its toxic concentration of 50 (1.891 mM). In summary, these findings combined with alginate’s biocompatibility and biodegradability give the combination solid potential in antimicrobial use.

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“…Several researchers also investigated the antimicrobial efficacy of metal oxide nanoparticles incorporated into alginate impression materials. They reported that these nanoparticles could be considered effective self-disinfecting agents for alginate impression materials with no adverse effect on the physical and mechanical properties [46,47].…”

Section: Discussionmentioning

confidence: 99%

Mechanical and Physical Properties of an Experimental Chemically and Green-Nano Improved Dental Alginate after Proven Antimicrobial Potentials

Singer

Bourauel

2023

Gels

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Objectives: Impression materials could be a source of cross-contamination due to the presence of microorganisms from blood and saliva inside the oral cavity. Nevertheless, routinely performed post-setting disinfection could compromise the dimensional accuracy and other mechanical properties of alginates. Thus, this study aimed to evaluate detail reproduction, dimensional accuracy, tear strength, and elastic recovery of new experimentally prepared self-disinfecting dental alginates. Methods: Two antimicrobial-modified dental alginate groups were prepared by mixing alginate powder with 0.2% silver nitrate (AgNO3 group) and a 0.2% chlorohexidine solution (CHX group) instead of pure water. Moreover, a third modified group was examined by the extraction of Boswellia sacra (BS) oleoresin using water. The extract was used to reduce silver nitrate to form silver nanoparticles (AgNPs), and the mixture was used as well in dental alginate preparation (BS + AgNP group). Dimensional accuracy and detail reproduction were examined as per the ISO 1563 standard guidelines. Specimens were prepared using a metallic mold engraved with three parallel vertical lines 20, 50, and 75 µm wide. Detail reproduction was evaluated by checking the reproducibility of the 50 µm line using a light microscope. Dimensional accuracy was assessed by measuring the change in length between defined reference points. Elastic recovery was measured according to ISO 1563:1990, in which specimens were gradually loaded and then the load was released to allow for recovery from the deformation. Tear strength was evaluated using a material testing machine until failure at a crosshead speed of 500 mm/min. Results: The recorded dimensional changes between all tested groups were insignificantly different and within the reported acceptable values (between 0.037–0.067 mm). For tear strength, there were statistically significant differences between all tested groups. Groups modified with CHX (1.17 ± 0.26 N/mm) and BS + AgNPs (1.11 ± 0.24 N/mm) showed higher tear strength values compared to the control (0.86 ± 0.23 N/mm) but were insignificant from AgNO3 (0.94 ± 0.17 N/mm). All tested groups showed elastic recovery values that met both the ISO standard and ADA specifications for elastic impression materials and tear strength values within the acceptable documented ranges. Discussion: The CHX, silver nitrate, and green-synthesized silver nanoparticles could be promising, inexpensive alternatives for the preparation of a self-disinfecting alginate impression material without affecting its performance. Green synthesis of metal nanoparticles could be a very safe, efficient, and nontoxic method, with the advantage of having a synergistic effect between metal ions and active chemical constituents of plant extracts.

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Comparative evaluation of efficacy of Zinc oxide and Copper oxide nanoparticles as antimicrobial additives in alginate impression materials

Cited by 14 publications

References 27 publications

Evaluation of Antimicrobial and Antibiofilm Activities of Copper Oxide Nanoparticles within Soft Denture Liners against Oral Pathogens

Evaluation of Antimicrobial and Antibiofilm Activities of Copper Oxide Nanoparticles within Soft Denture Liners against Oral Pathogens

Assessment of Antimicrobic, Antivirotic and Cytotoxic Potential of Alginate Beads Cross-Linked by Bivalent Ions for Vaginal Administration

Mechanical and Physical Properties of an Experimental Chemically and Green-Nano Improved Dental Alginate after Proven Antimicrobial Potentials

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