Comparative Evaluation of Direct Smear and Culture Methods for Detection of Trichomonas gallinae Infection in Pigeon and Chicken of Assam

Saikia, Munmi; Bhattacharjee, Kanta; Deka, Diganta; Sarmah, Prabhat Chandra

doi:10.47191/ijcsrr/v5-i11-31

Cited by 3 publications

(2 citation statements)

References 19 publications

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“…The rock-pigeon Columba is an old-world bird that is thought to be the sole source of T. gallinae (Lawson, 2010). Trichomoniasis is a potentially fatal disease that has been observed in numerous parts of the world and affects many birds, including hawks, owls, doves, pigeons, and other wild birds (Saikia, et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Genetic sequence and phylogenetic analysis of Trichomonos gallinae in racing pigeons at Mosul city, Iraq

Altememy,

Saeed

2023

Journal of Applied Veterinary Sciences

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This is the first study in Mosul to use genetic sequencing technology to diagnose and document the type, strain, and genotype of Trichomonos gallinae in racing pigeons. It was distinguished by the geographical sequence of Mosul, Iraqi city. Thirty isolates of T. gallinae were chosen from a total of 56 that had been molecularly characterized to examine the extent to which these isolates matched in terms of genetic sequencing. The DNA from the T. gallinae parasite was extracted, and the master mix for all of the polymerization reaction components was created based on the needed quantities of the reaction components for each sample. The acquired sequences were matched to known sequences in databases to determine the trichomonos species parasite and strain responsible for the infection. The results of the DNA sequencing examination revealed that after the polymerase chain reaction amplification products were sent to Macrogen, Korea, to determine the genetic variation of the local strains, the products of the small subunit rRNA-Gene and the reaction product of bp 194 of the Trichomonos gallinae parasite were sent to the National Centre for Biotechnology Information, NCBI Gen Bank, for recording. Based on the small partial ribosomal RNA according to blast in GenBank of the NCBI, the percentage of match in the genetic sequence was 100% between the genetic sequence in Mosul and the genetic sequences in Brazil and Portugal. France, Spain, Iran, Poland, Prague, Hungary, Australia, and the United States are among the countries involved. The Trichomonas gallinae genetic sequence in racing pigeons from Mosul has been discovered for the first time in the GenBank database, revealing a 100% match with other countries' sequences. This discovery reveals the pathogen's worldwide dissemination and interconnection, aiding in the development of effective diagnostic procedures, preventive measures, and targeted treatments. The discovery also emphasises the need for cooperation in monitoring and regulating the spread of the infection, supporting a collaborative strategy against avian diseases.

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Section: Introductionmentioning

confidence: 99%

Genetic sequence and phylogenetic analysis of Trichomonos gallinae in racing pigeons at Mosul city, Iraq

Altememy,

Saeed

2023

Journal of Applied Veterinary Sciences

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“…In vitro cultivation of T. gallinae was considered as the gold standard for detection of trichomonads due to easy interpretation even in poorly infected birds (Stabler, 1954) [3] . The comparative evaluation of direct smear (Giemsa stain) and culture methods for detection of Trichomonas gallinae infection in pigeon and chicken of Assam was carried out by Saikia et al (2022) [4] where it was concluded that culture of T. gallinae has been proved to be superior to wet mount preparation or staining methods in detection of the flagellate. Cell culture was adopted to differentiate between different strains of T. gallinae pathogenicity and it proved to be a sensitive tool (Honigberg et al, 1964) [5] T. gallinae could be cultured in a variety of media including liquid, semi liquid types and semi-solid CPLM-agar medium.…”

Section: Introductionmentioning

confidence: 99%

Comparative study of five culture media for in vitro culture of Trichomonas gallinae from birds in Assam

Saikia¹,

Bhattacharjee²,

PC³

et al. 2022

J. Entomol. Zool. Stud.

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A study was carried out in order to compare the in vitro efficacy of five different culture media against the flagellate protozoa, Trichomonas gallinae. For in vitro culture, oral swabs of T. gallinae were collected randomly from oropharynx and crop after visual inspection of birds as pigeon, duck, chicken and quail with a sterilized cotton swab and cultivated in five media namely modified Diamond media, Medium199, Minimum Essential Medium (MEM), RPMI 1640 and Nutrient broth. The initial inoculation of trophozoites into each media was 1X10 3 cell/ml. Among all the five media, Medium 199 showed the highest growth rate and motility of the organisms till 7 days after initial inoculation. All media were found suitable for harvesting and sub culture of T. gallinae under in vitro condition.

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Detection of Chicken Disease Based on Day-Age Using Pre Trained Model of CNN

Sreenivasulu,

Khan,

Damini

et al. 2023

Lecture Notes in Computer Science

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Comparative Evaluation of Direct Smear and Culture Methods for Detection of Trichomonas gallinae Infection in Pigeon and Chicken of Assam

Cited by 3 publications

References 19 publications

Genetic sequence and phylogenetic analysis of Trichomonos gallinae in racing pigeons at Mosul city, Iraq

Genetic sequence and phylogenetic analysis of Trichomonos gallinae in racing pigeons at Mosul city, Iraq

Comparative study of five culture media for in vitro culture of Trichomonas gallinae from birds in Assam

Detection of Chicken Disease Based on Day-Age Using Pre Trained Model of CNN

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