Comparative Evaluation of Differentiation Potential of Menstrual Blood- Versus Bone Marrow- Derived Stem Cells into Hepatocyte-Like Cells

Abstract

Menstrual blood has been introduced as an easily accessible and refreshing stem cell source with no ethical consideration. Although recent works have shown that menstrual blood stem cells (MenSCs) possess multi lineage differentiation capacity, their efficiency of hepatic differentiation in comparison to other stem cell resources has not been addressed so far. The aim of this study was to investigate hepatic differentiation capacity of MenSCs compared to bone marrow-derived stem cells (BMSCs) under protocols developed by different concentrations of hepatocyte growth factor (HGF) and oncostatin M (OSM) in combination with other components in serum supplemented or serum-free culture media. Such comparison was made after assessment of immunophenotye, trans-differentiation potential, immunogenicity and tumorigeicity of these cell types. The differential expression of mature hepatocyte markers such as albumin (ALB), cytokeratin 18 (CK-18), tyrosine aminotransferase and cholesterol 7 alpha-hydroxylase activities (CYP7A1) at both mRNA and protein levels in differentiating MenSCs was significantly higher in upper concentration of HGF and OSM (P1) compared to lower concentration of these factors (P2). Moreover, omission of serum during differentiation process (P3) caused typical improvement in functions assigned to hepatocytes in differentiated MenSCs. While up-regulation level of ALB and CYP7A1 was higher in differentiated MenSCs compared to driven BMSCs, expression level of CK-18, detected level of produced ALB and glycogen accumulation were lower or not significantly different. Therefore, based on the overall comparable hepatic differentiation ability of MenSCs with BMSCs, and also accessibility, refreshing nature and lack of ethical issues of MenSCs, these cells could be suggested as an apt and safe alternative to BMSCs for future stem cell therapy of chronic liver diseases.

Hepatocyte differentiation of human induced pluripotent stem cells is modulated by stearoyl‐CoA desaturase 1 activity

“…The sequences of primers are presented in Table S1. The condition for GAPDH were adopted from the study by Khanjani et al (2014). Relative gene expression was normalized by the amount of GAPDH for each sample and all quantities were expressed as an x fold difference relative to control.…”

Hardystonite‐Coated Poly(l‐lactide) Nanofibrous Scaffold and Efficient Osteogenic Differentiation of Adipose‐Derived Mesenchymal Stem Cells

“…Quantitative real-time PCR was performed according to Khanjani et al (2014) (29). Quantitative real-time PCR was performed according to Khanjani et al (2014) (29).…”

“…The biological behavior of different substrates toward osteogenic differentiation of AMSCs was investigated through quantitative real-time PCR of alkaline phosphatase, osteonectin, and osteocalcin. Quantitative real-time PCR was performed according to Khanjani et al (2014) (29). Investigated genes and their related specific primers are demonstrated in Table 1 Genetic Analyzer (Applied Biosystems) was used to sequence the amplified genes.…”

Regeneration capacity of the endometrium: endometrial stem cells

“…Kemik ili¤i nakli olan hastalarda verici hücrele-rinin alıcının endometriumunda varlı¤ını göste-ren çalımalar bulunmaktadır. [36] Ancak verilen kemik ili¤i içerisinde çok sayıda miyeloid ve lenfoid hücrelerde bulundu¤undan verici kemik ili¤i kaynaklı kök hücrelerinden farklılamı endometrial hücrelerin tespitinde verici miyeloid ve lenfoid hücrelerinin ayırt edilmesi önem taımaktadır. Bu hücrelerin tespiti insan lökosit antijen (HLA) uyumsuz kemik ili¤i nakli yapılan olgularda yapılmıtır.…”

“…Bu hücrelerin tespiti insan lökosit antijen (HLA) uyumsuz kemik ili¤i nakli yapılan olgularda yapılmıtır. [36] Bu olguların endometriumlarında verici HLA'sına sahip hücreler gerçek zamanlı polimeraz zincir reaksiyonu (PZR) ve immünohistokimyasal yöntemlerle saptanmı ve yerleim yerleri de¤erlendirilmitir. Epitelial hücrelerin %48'inin ve stromal hücrelerin %52'sinin bu hücreler tarafından oluturuldu¤u saptanmıtır.…”