Comparative evaluation of different preservation methods for cyanobacterial strains

Esteves‐Ferreira, Alberto A.; Corrêa, Débora Machado; Carneiro, Antônio Policarpo Souza; Rosa, Rinamara Martins; Loterio, Robson Kriiger; Araújo, Wagner L.

doi:10.1007/s10811-012-9927-9

Cited by 15 publications

(3 citation statements)

References 45 publications

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“…After 28 days of growth, filaments were re-suspended in the culture via pipette mixing and 1 ml of culture was sampled. This aliquot was preserved in 10% v/v glycerol, based on previously described protocols (60)(61)(62). The 1 ml culture aliquot was centrifuged at 10,000g for five minutes until the culture was sufficiently pelleted, followed by removal of supernatant.…”

Section: Discussionmentioning

confidence: 99%

Niche formation and metabolic interactions result in stable diversity in a spatially structured cyanobacterial community

Duxbury

Raguideau

Rosko

et al. 2022

Preprint

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Cyanobacterial granules and aggregates can readily form in aquatic environments. The microbial communities found within and around these structures can be referred to as the cyanosphere, and can enable collective metabolic activities relevant to biogeochemical cycles. Cyanosphere communities are suggested to have different composition to that in the surrounding environment, but studies to date are mostly based on single time point samples. Here, we retrieved samples containing cyanobacterial granules from a freshwater reservoir and maintained a culture through sub-culture passages under laboratory conditions for over a year. We show that cyanobacteria-dominated granules form readily and repeatedly in this system over passages, and that this structure formation process seems to be associated with cyanobacterial motility. Performing longitudinal short-read sequencing over several culture passages, we identified a cyanosphere community comprising of 17 species with maintained population structure. Using long-read sequencing from two different time point samples, we have re-constructed full, circular genomes for 15 of these species and annotated metabolic functions within. This predicts several metabolic interactions among community members, including sulfur cycling and carbon and vitamin exchange. Using three individual species isolated from this cyanosphere, we provide experimental support for growth on carbon sources predicted to be secreted by the cyanobacterium in the system. These findings reinforce the view that the cyanosphere can recruit and maintain a specific microbial community with specific functionalities embedded in a spatially-organised microenvironment. The presented community will act as a key model system for further understanding the formation of the structured cyanosphere, its function and stability, and its metabolic contribution to biogeochemical cycles.

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Section: Discussionmentioning

confidence: 99%

Niche formation and metabolic interactions result in stable diversity in a spatially structured cyanobacterial community

Duxbury

Raguideau

Rosko

et al. 2022

Preprint

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“…In our study, 5% DMSO (except for the CB0101 strain), along with the EPS, also worked well for most of the strains. In another study, Esteves-Ferreira et al (2013) used five microalgae and cyanobacterial strains for cryopreservation and found 10% glycerol to be the most efficient CPA [ 36 ]. Some studies have demonstrated the effectiveness of CPAs when used in combination rather than alone.…”

Section: Discussionmentioning

confidence: 99%

Cryopreservation of Cyanobacteria and Eukaryotic Microalgae Using Exopolysaccharide Extracted from a Glacier Bacterium

et al. 2021

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Exopolysaccharide (EPS) has been known to be a good cryoprotective agent for bacteria, but it has not been tested for cyanobacteria and eukaryotic microalgae. In this study, we used EPS extracted from a glacier bacterium as a cryoprotective agent for the cryopreservation of three unicellular cyanobacteria and two eukaryotic microalgae. Different concentrations of EPS (10%, 15%, and 20%) were tested, and the highest concentration (20%) of EPS yielded the best growth recovery for the algal strains we tested. We also compared EPS with 5% dimethyl sulfoxide (DMSO) and 10% glycerol for the cryopreservation recovery. The growth recovery for the microalgal strains after nine months of cryopreservation was better than 5% DMSO, a well-known cryoprotectant for microalgae. A poor recovery was recorded for all the tested strains with 10% glycerol as a cryoprotective agent. The patterns of growth recovery for most of these strains were similar after 5 days, 15 days, and 9 months of cryopreservation. Unlike common cryopreservants such as DMSO or methanol, which are hazardous materials, EPS is safe to handle. We demonstrate that the EPS from a psychrotrophic bacterium helped in the long-term cryopreservation of cyanobacteria and microalgae, and it has the potential to be used as natural cryoprotective agent for other cells.

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“…Maintaining pure cultures using preservation methods is of high importance for biotechnological purposes [46]. The first step toward successful isolation is understanding and mimicking the naturally occurring environmental conditions [47].…”

Section: Isolation Of Cyanobacteria and Microalgaementioning

confidence: 99%

Exploring Marine Resources for Bioactive Compounds

et al. 2014

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Biodiversity in the seas is only partly explored, although marine organisms are excellent sources for many industrial products. Through close co-operation between industrial and academic partners, it is possible to successfully collect, isolate and classify marine organisms, such as bacteria, fungi, micro- and macroalgae, cyanobacteria, and marine invertebrates from the oceans and seas globally. Extracts and purified compounds of these organisms can be studied for several therapeutically and industrially significant biological activities, including anticancer, anti-inflammatory, antiviral, antibacterial, and anticoagulant activities by applying a wide variety of screening tools, as well as for ion channel/receptor modulation and plant growth regulation. Chromatographic isolation of bioactive compounds will be followed by structural determination. Sustainable cultivation methods for promising organisms and biotechnological processes for selected compounds can be developed, as well as biosensors for monitoring the target compounds. The (semi)synthetic modification of marine-based bioactive compounds produces their new derivatives, structural analogs and mimetics that could serve as hit or lead compounds and be used to expand compound libraries based on marine natural products. The research innovations can be targeted for industrial product development in order to improve the growth and productivity of marine biotechnology. Marine research aims at a better understanding of environmentally conscious sourcing of marine biotechnology products and increased public awareness of marine biodiversity. Marine research is expected to offer novel marine-based lead compounds for industries and strengthen their product portfolios related to pharmaceutical, nutraceutical, cosmetic, agrochemical, food processing, material and biosensor applications.

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Comparative evaluation of different preservation methods for cyanobacterial strains

Cited by 15 publications

References 45 publications

Niche formation and metabolic interactions result in stable diversity in a spatially structured cyanobacterial community

Niche formation and metabolic interactions result in stable diversity in a spatially structured cyanobacterial community

Cryopreservation of Cyanobacteria and Eukaryotic Microalgae Using Exopolysaccharide Extracted from a Glacier Bacterium

Exploring Marine Resources for Bioactive Compounds

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