Comparative evaluation of different methods for determining phytochemicals and antioxidant activity in products containing betalains – Verification of beetroot samples

Kusznierewicz, Barbara; Mróz, Marika; Koss-Mikołajczyk, Izabela; Namieśnik, Jacek

doi:10.1016/j.foodchem.2021.130132

Cited by 38 publications

(47 citation statements)

References 32 publications

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“…The precursor ion of m / z 903.2291 represented the protonated mass ([M + H] + ) of C 40 H 42 N 2 O 22 with a mass accuracy of −1.55 ppm. The fragment of m / z 551.1499 [M + H–352.0792] + corresponded to the neutral loss of a feruloyl-glucuronosyl moiety (−352.0797 Da) as well as the signal of betanidin ( m / z 389.0972) and its characteristic fragment of aglycone ( m / z 150.0548); these results are consistent with a previous report …”

Section: Resultssupporting

confidence: 91%

“…Therefore, compound 38 was identified as alanine-betaxanthin, which has been identified in beetroots . The MS/MS spectrum of compound 26 showed the precursor ion at m / z 340.1136 [M + H] + , calculated as C 14 H 17 N 3 O 7 (Figure G), which is consistent with vulgaxanthin I in the literature . Vulgaxanthin I is biosynthesized from glutamine and betalamic acid.…”

Section: Resultssupporting

confidence: 76%

“…De novo structure elucidation of small molecules using HRMS is a well-established and powerful technology for the identification of natural products . A previous study showed that 48 betalains could be identified in beetroot using Orbitrap via the database . In the present study, we took advantage of accurate mass to infer the chemical formula, and then, we validated the formula by both mass accuracy and natural isotope abundance information.…”

Section: Resultsmentioning

confidence: 84%

“…32 Kusznierewicz et al reported 48 betalains in beetroot based on HRMS quadrupole-Orbitrap, but the MS2 information was not shown. 33 Our study comprehensively and simultaneously analyzed 68 betalains by Orbitrap, the precursors of accurate mass and high-resolution MS2 information were all provided in Table 1. A total of 68 betalains were further identified according to accurate mass, MS/MS fragments, and UV−vis spectra.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Comprehensive Betalain Profiling of Djulis (Chenopodium formosanum) Cultivars Using HPLC-Q-Orbitrap High-Resolution Mass Spectrometry

Xie

Chen

2021

J. Agric. Food Chem.

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Betalains are water-soluble pigments that may be used as alternatives to artificial colorants in the food industry. In addition, the betalain profile may be of taxonomic significance to some higher plants. Djulis (Chenopodium formosanum) is an emerging crop grown by Taiwan aboriginal communities, and its various cultivars contain different betalain compositions. To develop an authenticity method for evaluating djulis cultivars, we performed comprehensive betalain profiling with HPLC-Q-Orbitrap high-resolution mass spectrometry (HRMS). An HRMS method for betalain annotation was developed and used to identify 68 betalains, including 10 betacyanins, 24 betacyanin derivatives, and 34 betaxanthins. The profile of betacyanins and their derivatives could be utilized to classify different cultivars of djulis, while betaxanthins were less informative. Furthermore, the betalain profiles were indicative of the geographic origin of djulis. This HRMS method and analytical platform for betalains may be helpful to guide further investigations of natural pigments in other plants.

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Section: Resultssupporting

confidence: 91%

Section: Resultssupporting

confidence: 76%

Section: Resultsmentioning

confidence: 84%

Section: ■ Results and Discussionmentioning

confidence: 99%

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Comprehensive Betalain Profiling of Djulis (Chenopodium formosanum) Cultivars Using HPLC-Q-Orbitrap High-Resolution Mass Spectrometry

Xie

Chen

2021

J. Agric. Food Chem.

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“…Earlier researchers reported that noodle colour with the addition of beet extract were decrease in lightness (L*) and increased in redness (a*) value with the increase in concentration of beetroot addition [14,15]. Beetroot extract contain high amount of betanins as red pigment which have strong antioxidant activity to protect organisms against oxidative stress [16,17]. As shown in Table 3, the other physical characteristics were compression test, tensile strength, and elongation.…”

Section: Notementioning

confidence: 99%

Physical and Sensory Characteristic of Arenga-Arrowroot Starch Noodle with Beetroot Extract Addition

Herawati¹,

Ariani²,

Miftakhussolikhah³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Arrowroot tuber is plenty available in Indonesia as it has been cultivated widely. Arrowroot flour can be develop in noodle processing combine with arenga starch with ratio 75% : 25%. The color characteristic of the noodle was a bright brown-opaque color character, thus, make this noodle is not perceived well by customer. Addition of natural color, i.e. beetroot extract can improve the physical appearance. The purpose of this research was to identify the characteristic (physical and sensory) of the arenga-arrowroot starch noodle with beetroot extract addition. Beetroot extract were added in five different concentrations, namely 20%; 30%; 40%; 50%; and 60% w/w. A completely randomized design (CRD) with three repetitions was utilized in the experiment. Color, compression strength, tensile strength, and elongation were all tested physically. Sensory characteristic were analyzed using hedonic test with color, aroma, taste, and overal acceptance parameter. The findings revealed that the inclusion of beetroot extract has an effect on the physical and sensory properties of arenga starch - arrowroot flour noodle. The color brightness, compression test, elongation, and tensile strength of the arenga starch-arrowroot flour noodle would all be reduced if beetroot extract was added. The best sensory characteristic was reach by 20% w/w beetroot extract.

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Hypericum alpestre extract exhibits in vitro and in vivo anticancer properties by regulating the cellular antioxidant system and metabolic pathway of L‐arginine

Ginovyan,

Javrushyan,

Karapetyan

et al. 2024

Cell Biochemistry & Function

Self Cite

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Conventional treatment methods are not effective enough to fight the rapid increase in cancer cases. The interest is increasing in the investigation of herbal sources for the development of new anticancer therapeutics. This study aims to investigate the antitumor capacity of Hypericum alpestre (H. alpestre) extract in vitro and in vivo, either alone or in combination with the inhibitors of the l‐arginine/polyamine/nitric oxide (NO) pathway, and to characterize its active phytochemicals using advanced chromatographic techniques. Our previous reports suggest beneficial effects of the arginase inhibitor NG‐hydroxy‐nor‐ l‐arginine and NO inhibitor NG‐nitro‐Larginine methyl ester in the treatment of breast cancer via downregulation of polyamine and NO synthesis. Here, the antitumor properties of H. alpestre and its combinations were explored in vivo, in a rat model of mammary gland carcinogenesis induced by subcutaneous injection of 7,12‐dimethylbenz[a]anthracene. The study revealed strong antiradical activity of H. alpestre aerial part extract in chemical (DPPH/ABTS) tests. In the in vitro antioxidant activity test, the H. alpestre extract demonstrated pro‐oxidant characteristics in human colorectal (HT29) cells, which were contingent upon the hemostatic condition of the cells. The H. alpestre extract expressed a cytotoxic effect on HT29 and breast cancer (MCF‐7) cells measured by the MTT test. According to comet assay results, H. alpestre extract did not exhibit genotoxic activity nor possessed antigenotoxic properties in HT29 cells. Overall, 233 substances have been identified and annotated in H. alpestre extract using the LC‐Q‐Orbitrap HRMS system. In vivo experiments using rat breast cancer models revealed that the H. alpestre extract activated the antioxidant enzymes in the liver, brain, and tumors. H. alpestre combined with chemotherapeutic agents attenuated cancer‐like histological alterations and showed significant reductions in tumor blood vessel area. Thus, either alone or in combination with Nω‐OH‐nor‐ l‐arginine and Nω‐nitro‐ l‐arginine methyl ester, H. alpestre extract exhibits pro‐ and antioxidant, antiangiogenic, and cytotoxic effects.

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Comparative evaluation of different methods for determining phytochemicals and antioxidant activity in products containing betalains – Verification of beetroot samples

Cited by 38 publications

References 32 publications

Comprehensive Betalain Profiling of Djulis (Chenopodium formosanum) Cultivars Using HPLC-Q-Orbitrap High-Resolution Mass Spectrometry

Comprehensive Betalain Profiling of Djulis (Chenopodium formosanum) Cultivars Using HPLC-Q-Orbitrap High-Resolution Mass Spectrometry

Physical and Sensory Characteristic of Arenga-Arrowroot Starch Noodle with Beetroot Extract Addition

Hypericum alpestre extract exhibits in vitro and in vivo anticancer properties by regulating the cellular antioxidant system and metabolic pathway of L‐arginine

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