Comparative Evaluation of Celsior Solution Versus Viaspan in a Pig Liver Transplantation Model1

Audet, Maxime; Alexandre, Eliane; Mustun, Ashiq; David, P; Chenard-Neu, Marie-Pierre; Tiollier, Jérôme; Jaeck, Daniel; Cinqualbre, J; Wolf, P; Boudjéma, Karim

doi:10.1097/00007890-200106270-00005

Cited by 28 publications

(21 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In contrast to data from a study with CS in a rat liver-transplantation model, our results from intracellular ATP failed to confirm a significantly higher level after the ischemia phase [36]. However, the results of ATP level after a consecutive reperfusion phase were in accordance with this rat-liver model and another recently performed study from pig-liver transplantation [2]. We assume that the use of different experimental models (human/animal) in each study may explain this finding, as is supposed by others [30].…”

Section: Discussioncontrasting

confidence: 99%

Celsior solution compared with University of Wisconsin solution (UW) and histidine-tryptophan-ketoglutarate solution (HTK) in the protection of human hepatocytes against ischemia-reperfusion injury

Janssen¹,

Janbetaen²,

Broelsch³

2003

Transplant Int

View full text Add to dashboard Cite

Celsior, a new preservation solution in thoracic organ transplantation was evaluated for efficacy in cold preservation of human hepatocytes and compared with University of Wisconsin solution (UW) and histidine-tryptophan-ketoglutarate solution (HTK, Custodiol). Human hepatocyte cultures were preserved at 4 "C in Celsior, UW and HTK for 2, 6, 12, 24 and 48 h with 6 h of reperfusion. Levels of lactate dehydrogenase (LDH; cell necrosis), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; mitochondria1 function), and adenosine 5'-triphosphate (ATP; loss of intracellular energy) were measured. Cell necrosis, mitochondrial dysfunction, and loss of ATP were significantly (P < 0.001, P < 0.001, P < 0.002, respectively) lower in Celsior than in HTK. The amount of cell necrosis and mitochondrial dysfunction in Celsior solution (CS) and UW was equal (P = n.s.) up to 24 h and significantly lower in UW after 48 h (P< 0.001).Additionally, the intracellular level of ATP was significantly higher after ischemia (P < 0.001) and reperfusion from long-term ischemia (24, 48 h) (P < 0.002). We can conclude that Celsior was superior to HTK and equal to UW in the protection of human hepatocytes against cold preservation injury from ischemia and reperfusion. Furthermore, Celsior was effective in long-term preservation of human hepatocytes.

show abstract

Section: Discussioncontrasting

confidence: 99%

Celsior solution compared with University of Wisconsin solution (UW) and histidine-tryptophan-ketoglutarate solution (HTK) in the protection of human hepatocytes against ischemia-reperfusion injury

Janssen¹,

Janbetaen²,

Broelsch³

2003

Transplant Int

View full text Add to dashboard Cite

show abstract

“…19 Some preliminary reports recently suggested that CS is effective for preserving not only thoracic organs, but also livers, in both the preclinical and clinical settings. [20][21][22][23] According to the experience of Audet et al 24 in a pig model of OLT, liver grafts perfused with CS performed similarly to those perfused with UW solution in terms of postoperative animal and graft survival. Ohwada et al 25 report better results with liver grafts perfused with CS than with UW solution in a canine OLT model based on non-heart-beating donors.…”

Section: Discussionmentioning

confidence: 99%

A multicenter pilot prospective study comparing Celsior and University of Wisconsin preserving solutions for use in liver transplantation

Cavallari

Cillo

Nardo

et al. 2003

Liver Transplantation

View full text Add to dashboard Cite

Primary dysfunction (PDF) still occurs after orthotopic liver transplantation (OLT). Celsior solution (CS) might offer some advantages over the conventional University of Wisconsin (UW) solution for organ preservation, but to date, this has not been prospectively evaluated in the context of OLT. In this prospective, randomized, multicenter, pilot study, 215 potential liver donors were enrolled and randomized. In 42 cases, the livers were unsuitable for transplantation; therefore, 173 randomized livers ultimately were implanted after perfusion and cold preservation with CS (n ‫؍‬ 83) or UW solution (n ‫؍‬ 90). In accord with the indications of the CS manufacturing company, total CS infusion volume was 90 mL/kg, greater than that of UW solution (60 mL/kg). The main aim of the study is to compare the prevalence of PDF between the two groups. Donor and recipient variables were similar in the two groups. Episodes of PDF were numerically lower in the CS (2.4%) than UW group (7.8%), but the difference was not statistically significant. There was a trend toward a lesser need for early re-OLT (<30 days) in the CS group (P ‫؍‬ .0507), but again, no statistically significant difference emerged. Overall and time-differentiated postoperative deaths also were similar. One-year actuarial patient (UW, 89% v CS, 87%) and graft (UW, 83% v CS, 85%) survival rates were similar. In conclusion, CS was similar to UW solution as a preservation solution in the clinical setting of OLT at the infusion volumes described, although some theoretical advantages of CS composition suggest that CS might prove a valid alternative to UW preservation solution in multiorgan harvesting, including the liver. A study on a larger patient basis is needed. (Liver Transpl 2003;9:814-821.)

show abstract

“…The advantage of organ preservation solutions lies in their ability to prevent tissue acidosis, cell swelling, free radical damage, and energy depletion, all phenomena which occur during ischemia and subsequent reperfusion. A relatively new organ preservation solution, called Celsior (Cs) solution, has also shown to be effective in both experimental and clinical organ preservation [14][15][16][17][18][19][20][21]. It was originally designed for donor heart and lung preservation but has also proven to be suitable for the preservation of liver grafts [15,18,21].…”

Section: Introductionmentioning

confidence: 99%

Hypothermic in situ perfusion of the porcine liver using Celsior or Ringer-lactate solution

Dinant

Roseboom

Levi

et al. 2008

Langenbecks Arch Surg

View full text Add to dashboard Cite

Background Hypothermic perfusion (HP) of the liver is applied during total vascular exclusion (TVE) to reduce ischemic injury during liver resection. No studies have been performed comparing different perfusion solutions for HP. The aim of this experimental study was to compare Ringerlactate solution (RL) with Celsior solution (Cs) for HP in a pig model of 60-min TVE. Method Twenty pigs underwent 60-min TVE of the liver. Groups were TVE without HP (no-HP, n=9), TVE with HP using RL (n=6), and TVE with HP using Cs (n=5). Blood and liver tissue samples were taken before TVE and during 24-h reperfusion. Results In the no-HP group, plasma aspartate aminotransferase values were significantly increased during reperfusion (p<0.05), while liver tissue pO 2 levels (p< 0.01) were decreased when compared to the HP groups. After 24-h reperfusion, bile production and liver tissue glutathione content were significantly higher (p<0.05) in the Cs group (42.0±1.7 mL/h and 44.9±2.2 nmol/mg, respectively) as compared to the RL group (31.5±3.5 mL/h and 19.6±1.8 nmol/mg, respectively). ConclusionThe protective effect of HP during TVE was confirmed in this study. HP with Cs was more effective in reducing ischemic injury as compared to HP with RL.

show abstract

Comparative Evaluation of Celsior Solution Versus Viaspan in a Pig Liver Transplantation Model1

Abstract: Within the limits of this pilot study, CS is equivalent to UW in terms of graft function and animal survival.

Cited by 28 publications

References 18 publications

Celsior solution compared with University of Wisconsin solution (UW) and histidine-tryptophan-ketoglutarate solution (HTK) in the protection of human hepatocytes against ischemia-reperfusion injury

Celsior solution compared with University of Wisconsin solution (UW) and histidine-tryptophan-ketoglutarate solution (HTK) in the protection of human hepatocytes against ischemia-reperfusion injury

A multicenter pilot prospective study comparing Celsior and University of Wisconsin preserving solutions for use in liver transplantation

Hypothermic in situ perfusion of the porcine liver using Celsior or Ringer-lactate solution

Contact Info

Product

Resources

About