Comparative Evaluation of Biomed InTray® Colorex MRSA with BD ESwab Collection Kit/ BBL™ CHROMagar® MRSA II

Bijlani, Kiran; Gomez, M Fuentes; Matias, Rowena D.; Najafi, Ana; Najafi, Ramin; Arumugam, Selva Sharma

doi:10.4172/2161-0703.1000273

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“…These in-vitro culture-media cassettes have several advantages: FDA and CE-marked, ready-to-use, small size for easy transport and storage and long shelf-life even with blood-containing media (12 months for most agar formulations). They can also be customised with nearly any media and are recommended for the isolation, cultivation and counting of fastidious and non-fastidious organisms [14,15]. For this evaluation, firstly, we performed a proof-of-concept study with the following objectives: (a) test the use of InTray cassettes directly from blood cultures, and (b) select the most convenient media for our application.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media

et al. 2023

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Culture media is fundamental in clinical bacteriology for the detection and isolation of bacterial pathogens. However, in-house media preparation could be challenging in low-resource settings. InTray® cassettes (Biomed Diagnostics) could be a valid alternative as they are compact, ready-to-use media preparations. In this study, we evaluate the use of two InTray media as a subculture alternative for the diagnosis of bloodstream infections: the InTray® Müller-Hinton (MH) chocolate and the InTray® Colorex™ Screen. The InTray MH chocolate was evaluated in 2 steps: firstly, using simulated positive blood cultures (reference evaluation study), and secondly, using positive blood cultures from a routine clinical laboratory (clinical evaluation study). The Colorex Screen was tested using simulated poly-microbial blood cultures. The sensitivity and specificity of the InTray MH chocolate were respectively 99.2% and 90% in the reference evaluation study and 97.1% and 88.2% in the clinical evaluation study. The time to detection (TTD) was ≤20 h in most positive blood cultures (99.8% and 97% in the two studies, respectively). The InTray® MH Chocolate agar showed good performance when used directly from clinical blood cultures for single bacterial infections. However, mixed flora is more challenging to interpret on this media than on Colorex™ Screen, even for an experienced microbiologist.

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Section: Introductionmentioning

confidence: 99%