Comparative evaluation of BACTEC FX, BacT/ALERT 3D, and BacT/ALERT VIRTUO automated blood culture systems using simulated blood cultures

Li, Zhengkang; Liu, Suling; Chen, Huimin; Zhang, Xinqiang; Ling, Yong; Zhang, Ni; Hou, Tieying

doi:10.1080/17843286.2020.1797343

Cited by 10 publications

(6 citation statements)

References 22 publications

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“…The AccuMed ESP-384 system with a 3-day incubation was considered sufficient, with 94.2% of positives detected ( 23 ). There are reports of the Virtuo system having an even faster time to positivity compared to other blood culture systems—BD Bactec FX ( 6 , 24 , 25 ) and the BacT/Alert 3D system ( 8 – 10 , 24 , 26 – 28 )—although the vast majority of these investigations used seeded studies. It should be noted that the epidemiological prevalence of species throughout these studies was similar to that in the present study.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Optimal Blood Culture Incubation Time To Maximize Clinically Relevant Results from a Contemporary Blood Culture Instrument and Media System

et al. 2021

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Timely diagnosis of microorganisms in blood cultures is necessary to optimize therapy. Although blood culture media and systems have evolved for decades, the standard interval for incubation prior to discard as negative has remained five days. Here, we evaluated the optimal incubation time for the BACT/ALERT VIRTUO blood culture detection system (bioMérieux) using FA Plus (aerobic) and FN Plus (anaerobic) resin culture bottles in routine clinical use. Following IRB approval, a retrospective review evaluated the outcomes of 158,710 bottles collected between November 2018 and October 2019. The number of positive blood bottles was 13,592 (8.6%); 99% of positive aerobic and anaerobic bottles flagged positive by 91.5 h and 108 h, respectively. The mean (median) time-to-positivity for Staphylococcus aureus was 18.4 h (15.6 h), Escherichia coli 12.3 h (9.5 h), Pseudomonas aeruginosa 22.2 h (15.9 h), and Candida spp. 48.9 h (42.9 h). Only 175 bottles (0.1% of all bottles) flagged positive after four days of incubation; 89 (51%) of these bottles grew Cutibacterium (Propionibacterium) species. Chart review of blood cultures positive after four days (96 h) rarely had clinical impact, and sometimes had a negative impact on patientcare. Finally, a seeded study of the HACEK group, historically associated with delayed blood culture positivity, demonstrated no benefit to extended incubation beyond four days. Collectively, these findings demonstrated that a four-day incubation time was sufficient for the VIRTUO system and media. Implementation of the four-day incubation time could enhance clinically relevant results by reducing recovery of contaminants and finalizing blood cultures one day earlier.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Optimal Blood Culture Incubation Time To Maximize Clinically Relevant Results from a Contemporary Blood Culture Instrument and Media System

et al. 2021

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“…In summary, automated methodologies manifest several advantages regarding the STTP when compared to manual methods [73]. Therefore, despite the manual methodology being applied in this work, the positivity rate is similar to the times resulting from automated methodologies [20,29].…”

Section: Profile Of Internal Pressure Variation In Blood Culture Flasks According To Different Groups Of Microorganisms X Timementioning

confidence: 74%

“…The automated blood culture methodologies, considered the most efficient and used worldwide, demonstrate advantages in terms of specificity, sensitivity and an average positivity time of 24 h [16][17][18]. However, microbiology laboratories with no financial resources are at a disadvantage because they need monitoring equipment that has a high cost of acquisition and maintenance [18][19][20]. Manual methodologies are thus used as an alternative.…”

Section: Introductionmentioning

confidence: 99%

Development of a Manometric Monitoring Method for Early Detection of Air Microbiological Contamination in the Bloodstream

Neto

Pinheiro

Santos³

et al. 2021

Atmosphere

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Atmospheric air is a microbial habitat of pathogenic bioaerosols that may pose serious risks to humans. A commonly laboratory-based approach for the diagnosis of such infections in the bloodstream is the blood culture analysis. Its clinical relevance is attributed to the fact that these infections are characterized by high rates of morbidity and mortality, requiring the need for efficient methods for rapid diagnosis. For this reason, our study aimed to develop a method of manometric monitoring for the rapid detection of viable microorganisms in blood culture vials. A methodology was developed to detect pressure variation in intra-vials through a manometric instrument that was coupled to vials of blood culture containing culture broth that allowed microbial growth. This device allowed the early detection of microbial activity based on the production or use of intra-flask gases as a result of microbial metabolic activity. The analyzed variables were the pressure as a function of time, microbial species, and culture medium. The highest pressure found in the flasks without microorganisms was 40 mmHg between 2 and 6 h, and the lowest pressure was −42 mmHg between 21 and 24 h. The variation of the internal pressure in blood culture flasks according to different groups of microorganisms as a function of time demonstrated that the fermentative gram-negative bacilli and gram-positive cocci exhibited a significant increase in relation to their respective control groups (p < 0.001). The non-fermenting gram-negative bacilli showed expected results in relation to the pressure variation in which the production of negative pressures was noticed during the period of analysis, with a significant difference with respect to their control groups (p < 0.001). The developed methodology for the early detection of microorganisms responsible for bloodstream infection was demonstrated to be effective.

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“…Historically, blood cultures were manual and time-consuming; but with the advent of automated monitoring instruments, time to positivity has decreased. BacT/Alert Virtuo, a more advanced version of automated monitoring instrument, has been shown to have lower TTP than other current instruments ( 3 – 8 ). In this study, we examined the impact of implementing the Virtuo instrument on TTP in an ICU patient population at a multicenter hospital setting.…”

Section: Observationmentioning

confidence: 99%

Impact of Implementation of BacT / Alert Virtuo on Blood Culture Time to Positivity in Sepsis Patients

et al. 2023

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Comparative evaluation of BACTEC FX, BacT/ALERT 3D, and BacT/ALERT VIRTUO automated blood culture systems using simulated blood cultures

Cited by 10 publications

References 22 publications

Evaluation of Optimal Blood Culture Incubation Time To Maximize Clinically Relevant Results from a Contemporary Blood Culture Instrument and Media System

Evaluation of Optimal Blood Culture Incubation Time To Maximize Clinically Relevant Results from a Contemporary Blood Culture Instrument and Media System

Development of a Manometric Monitoring Method for Early Detection of Air Microbiological Contamination in the Bloodstream

Impact of Implementation of BacT / Alert Virtuo on Blood Culture Time to Positivity in Sepsis Patients

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