Comparative evaluation of antimutagenic and antimitotic effects of<i>Morchella esculenta</i>extracts and protocatechuic acid

Stojković, Dejan; Davidović, Slobodan; Živković, Jelena; Glamočlija, Jasmina; Ćirić, Ana; Stevanović, Milena; Ferreira, Isabel C.F.R.; Sokóvić, Marina

doi:10.1080/21553769.2014.901925

Cited by 11 publications

(2 citation statements)

References 31 publications

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“…Protocatechuic acid or 3,4-dihydroxybenzoic acid (3,4-DHBA) is a catechol-type phenol present in some fruits and vegetables [1,2]. It has been shown that 3,4-DHBA displays antibacterial [3], antimutagenic [4], anti-inflammatory [5], antihyperglycemic [6] and highly antioxidant [7] effects. It also has potential applications for further microbiological synthesis of numerous valuable compounds, including the bioplastic precursor cis,cis-muconic acid [8,9].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid

et al. 2020

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The dehydroshikimate dehydratase (DSD) from Corynebacterium glutamicum encoded by the qsuB gene is related to the previously described QuiC1 protein (39.9% identity) from Pseudomonas putida. Both QuiC1 and QsuB are two-domain bacterial DSDs. The N-terminal domain provides dehydratase activity, while the C-terminal domain has sequence identity with 4-hydroxyphenylpyruvate dioxygenase. Here, the QsuB protein and its N-terminal domain (N-QsuB) were expressed in the T7 system, purified and characterized. QsuB was present mainly in octameric form (60%), while N-QsuB had a predominantly monomeric structure (80%) in aqueous buffer. Both proteins possessed DSD activity with one of the following cofactors (listed in the order of decreasing activity): Co 2+ , Mg 2+ , Mn 2+. The K m and k cat values for the QsuB enzyme (K m~1 mM, k cat~6 1 s-1) were two and three times higher than those for N-QsuB. 3,4-DHBA inhibited QsuB (K i~0 .38 mM, K i '~0.96 mM) and N-QsuB (K i~0 .69 mM) enzymes via mixed and noncompetitive inhibition mechanism, respectively. E. coli MG1655ΔaroEP lac-qsuB strain produced three times more 3,4-DHBA from glucose in test tube fermentation than the MG1655ΔaroEP lac-n-qsuB strain. The C-terminal domain activity towards 3,4-DHBA was not established in vitro. This domain was proposed to promote protein oligomerization for maintaining structural stability of the enzyme. The dimer formation of QsuB protein was more predictable (ΔG =-15.8 kcal/mol) than the dimerization of its truncated version N-QsuB (ΔG =-0.4 kcal/mol).

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Section: Introductionmentioning

confidence: 99%

Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid

et al. 2020

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“…Caffeine showed mutagenic properties in cells but not in mammalians [ 91 ] and EFSA evaluated safe intake values [ 50 ], kaempferol also showed an absence of adverse effects [ 92 , 93 ]. For chlorogenic and caffeic acid [ 94 ] antimutagenic effects were found, and for protocatechuic acid [ 95 ] negative results of the mutagenic potential are available. For coffee itself, in summary, negative results were found [ 96 ] in animals, but not in the in vitro tests.…”

Section: Discussionmentioning

confidence: 99%

Toxicological Assessment of Roasted Coffee Silver Skin (Testa of Coffea sp.) as Novel Food Ingredient

Lorbeer

Schwarz²,

Franke

et al. 2022

Molecules

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Roasted coffee silver skin is a coffee by-product, the uses of which are currently limited, e.g., as fertilizer, for energy production, or animal feed. Due to a low content of fat and carbohydrates combined with a high content of fiber, polyphenols and proteins, roasted silver skin is a valuable possible food ingredient. Potential applications include partial flour replacement in bakery products, as antioxidant and providing protein or fiber sources in sports or functional foods. As no relevant consumption of isolated silver skin occurred before 1997 in the European Union (EU), it was classified as a novel food in need of premarketing approval. Novel food applications must meet legal requirements for compositional and toxicological information. This review presents information on silver skin composition and toxicological studies. Several in vitro studies and subchronic in vivo studies are available with negative results, not suggesting a need for further studies on carcinogenic effects, reproduction, or chronic toxicity. All available studies so far concluded that no toxic effects of silver skin were found or are to be expected. For a novel food application in the EU, further in vitro studies on mutagenic potential may be needed to close a formal data gap.

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Role of the Gut Flora in Human Nutrition and Gut Health

Alvarado-Martinez

Filho

Mihalik

et al. 2020

Gut Microbiome and Its Impact on Health and Diseases

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Comparative evaluation of antimutagenic and antimitotic effects ofMorchella esculentaextracts and protocatechuic acid

Cited by 11 publications

References 31 publications

Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid

Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid

Toxicological Assessment of Roasted Coffee Silver Skin (Testa of Coffea sp.) as Novel Food Ingredient

Role of the Gut Flora in Human Nutrition and Gut Health

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