Comparative Evaluation of Alpha-Amylase and Alpha- Glucosidase Inhibitory Potential of Aqueous Seed Extract of Trigonella Foenum-Graecum and Moringa Oleifera - An In vitro Study

Abstract

Background: Trigonella foenum-graecum is one of the commonly used herbs in food. Moringa oleifera is a source of food, accommodation and conventional medicine for many peoples in the developing countriesThe seeds of both the plants were explored for antidiabetic potential.
 Methods: The current work was designed to probe the in vitro anti diabetic potential of the aqueous seed elicit of trigonella foenum- graceus and Moringa oleifera using the enzymes alpha amylase and alpha glucosidase.Both the extracts were screened for their phytochemicals and antioxidant potential was also analysed.The data were examine statistically by a one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test was used to see the statistical significance in conjunction with groups. The results with the p<0.05 level were considered to be statistically significant.
 Result: Due to its chemical ingredient and active compounds like amino acids, alkaloids, flavonoids, it proceeds as a good antioxidant. Trigonella foenum-graecum seed extract exhibited comparatively higher antidiabetic potential with an IC50 of 300µg/ml than the Moringa oleifera extract. Results of the work designate that both extracts of the plant possessed by forbidding alpha amylase, alpha glucosidase show maximum inhibition. Hence concluded that, Trigonella foenum graecum leaves might be considered as herbal remedies for diabetes.
 Conclusion: In vitro antioxidant and antidiabetic potential of seed elicit of Trigonella foenum-graecum and Moringa oleifera were analyzed and compared. This study shows that Trigonella foenum-graecum has higher antidiabetic potential than the Moringa oleifera extract.

Formulation and assessment of chickpea pulao using fenugreek seeds and Indian rennet to improve blood glycemic levels

“…A study conducted by Prithiksha et al. ( 2022 ) tested the α‐glucosidase activity of aqueous fenugreek seed extract, and the results indicated that α‐glucosidase inhibition activity of extracts (100–500 μg/mL) ranged from 21% to 58% linearly. Moreover, Ganeshpurkar et al.…”

“…Prithiksha et al (2022) tested the α-amylase activity of 80% aqueous fenugreek seed extract at different concentrations ranging from 100 to 500 μg/mL. Results depicted the linear relationship between concentrations and α-amylase inhibition as the % inhibition activity ranged from 30% at 100 μg/mL to 85% at 500 μg/mL Sunita (2020).…”

“…The IC 50 values were recorded as 14.98% and 16.24% for unboiled and boiled combined extracts, respectively.Various studies tested herbs for their α-glucosidase inhibition potential. A study conducted byPrithiksha et al (2022) tested the α-glucosidase activity of aqueous fenugreek seed extract, and the results indicated that α-glucosidase inhibition activity of extracts (100-500 μg/mL) ranged from 21% to 58% linearly. Moreover,Ganeshpurkar et al (2013) also measured α-glucosidase inhibition activity in methanolic and aqueous fenugreek extracts, and the results depicted that the aqueous extract showed maximum inhibition activity of 32% at the highest concentration (250 μg/mL), while the methanolic extract showed 53% inhibition activity at the same concentration.…”