Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

Lade, Harshad; Kim, Jungmin; Chung, Yousun; Han, Minje; Mo, Eun-Kyung; Kim, Jae‐Seok

doi:10.3390/diagnostics12010009

Cited by 6 publications

(6 citation statements)

References 24 publications

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“…The two settings of testing (for inpatients and emergency department settings) were both available in both periods (pre and pandemic‐periods) and, evaluating the same pattern of respiratory viruses (with the only exeption of SARS‐CoV‐2, which was not considered in this study), their use should not have had any impact on respiratory virus detection changes before and during the pandemic. In terms of sensitivity, studies showed concordant performance of the AllPlex respiratory panels, QIAstat RP, Eplex, and the FilmArray panels for the simultaneous detection of multiple respiratory viruses 14–16 …”

Section: Methodsmentioning

confidence: 98%

“…In terms of sensitivity, studies showed concordant performance of the AllPlex respiratory panels, QIAstat RP, Eplex, and the FilmArray panels for the simultaneous detection of multiple respiratory viruses [14][15][16] 1. Children hospitalized in the covid period had a higher probability of being female (p = .012), having underlying diseases (p = .013) and signs and symptoms of dyspnea (p = .01), and lower respiratory tract abnormalities (e.g., chest retractions, p = .002).…”

Section: Kits For Respiratory Virusesmentioning

confidence: 96%

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Changes in clinical, demographic, and outcome patterns of children hospitalized with non‐SARS‐CoV‐2 viral low respiratory tract infections before and during the COVID pandemic in Rome, Italy

Buonsenso,

Ferro,

Viozzi

et al. 2023

Pediatric Pulmonology

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IntroductionWe performed this study aiming to evaluate changes in epidemiology, clinical presentation and outcomes of children hospitalized for viral lower respiratory tract infections (LRTI).MethodsWe performed a retrospective study of children younger than 18 years of age hospitalized for LRTIs with a positive respiratory viral testing from 2018 to 2022. We compared need of pediatric intensive care unit (PICU), invasive ventilation, and other respiratory support, viral etiologies, clinical presentations, imaging, and laboratory results in the precovid (2018–2019) and covid (2020–2022) period.ResultsA total of 523 were included in the analysis. In the pandemic period, the detection of influenza was 95% less likely to occur (odds ratio [OR]: 0.05; 95% confidence interval [95% CI]: 0.02–0.12; p < .001), likewise the detection of adenovirus was 77% less likely to occur (OR: 0.23; 95% CI: 0.10–0.51; p < .001). In the pandemic period, the number of codetections increased from 15.52% in 2018 to 57.25% in 2022, resulting in a significantly increasing trend (p < .001). The odds of transfer to PICU was more than five times greater during the pandemic period (OR: 5.31; 95% CI: 1.78–15.86; p = .003).ConclusionsWe found that the pattern of LRTI in children during COVID‐19 pandemic significantly changed in terms of etiologies and increased severity.

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Section: Methodsmentioning

confidence: 98%

Section: Kits For Respiratory Virusesmentioning

confidence: 96%

Changes in clinical, demographic, and outcome patterns of children hospitalized with non‐SARS‐CoV‐2 viral low respiratory tract infections before and during the COVID pandemic in Rome, Italy

Buonsenso,

Ferro,

Viozzi

et al. 2023

Pediatric Pulmonology

View full text Add to dashboard Cite

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“…Their findings showed that the concordance between the FilmArray TM system and viral culture was 94.5% [ 56 ]. Similarly, Lade H et al indicated that the FilmArray TM RP assay is easy to work and provide rapid identification of respiratory viruses [ 57 ]. Moreover, Tazi S et al [ 31 ] showed that the estimated sensitivity and specificity of FilmArray, compared with the MAScIR SARS-CoV-2 M kit 2.0, were 100% and 79.2%, respectively.…”

Section: Discussionmentioning

confidence: 99%

Distribution of Viral Respiratory Infections during the COVID-19 Pandemic Using the FilmArray Respiratory Panel

Chen

2022

Biomedicines

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This study was conducted to evaluate the distribution of respiratory viral pathogens in the emergency department during the coronavirus disease 2019 (COVID-19) pandemic. Between May 2020 and September 2022, patients aged between 0.1 and 98 years arrived at the emergency department of Asia University Hospital, and samples from nasopharyngeal swabs were tested by the FilmArrayTM Respiratory Panel (RP). SARS-CoV-2 positivity was subsequently retested by the cobas Liat system. There were 804 patients for whom the FilmArrayTM RP was tested, and 225 (27.9%) of them had positive results for respiratory viruses. Rhinovirus/enterovirus was the most commonly detected pathogen, with 170 (61.8%) cases, followed by adenovirus with 38 (13.8%), SARS-CoV-2 with 16 (5.8%) cases, and coronavirus 229E, with 16 (5.8%) cases. SARS-CoV-2 PCR results were positive in 16 (5.8%) cases, and there were two coinfections of SARS-CoV-2 with adenovirus and rhinovirus/enterovirus. A total of 43 (5.3%) patients were coinfected; the most coinfection was adenovirus plus rhinovirus/enterovirus, which was detectable in 18 (41.9%) cases. No atypical pathogens were found in this study. Intriguingly, our results showed that there was prefect agreement between the detection of SARS-CoV-2 conducted with the cobas Liat SARS-CoV-2 and influenza A/B nucleic acid test and the FilmArrayTM RP. Therefore, the FilmArrayTM RP assay is a reliable and feasible method for the detection of SARS-CoV-2. In summary, FilmArrayTM RP significantly broadens our capability to detect multiple respiratory infections due to viruses and atypical bacteria. It provides a prompt evaluation of pathogens to enhance patient care and clinical selection strategies in emergency departments during the COVID-19 pandemic.

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“…We used several panels: The two settings of testing (for inpatients and emergency department settings) were both available in both periods (pre-and pandemic-periods) and, evaluating the same pattern of respiratory viruses (with the only exception of SARS-CoV-2, which was not considered in this study), their use should not have had any impact on respiratory virus detection changes before and during the pandemic. In terms of sensitivity, studies showed concordant performance of the AllPlex respiratory panels, QIAstat RP, Eplex, and the FilmArray panels for the simultaneous detection of multiple respiratory viruses [8][9][10].…”

Section: Kits For Respiratory Virusesmentioning

confidence: 96%

Determinants of antibiotic prescription in children with adenovirus respiratory tract infections

Buonsenso,

Camporesi,

Viozzi

et al. 2024

Eur J Pediatr

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We performed this study to evaluate factors associated with antibiotic prescriptions in children with adenovirus infection, since no studies have attempted to address this aspect in the pediatric population. Retrospective study of children younger than 18 years of age tested positive for adenovirus on a syndromic nasopharyngeal test from 2018 to 2023. We compared the need of pediatric intensive care unit (PICU), invasive ventilation, and other respiratory support, viral etiologies, clinical presentations, imaging, and laboratory results in the precovid (2018–2019) and covid (2020–2022) period. The use of antibiotics was studied with multivariable logistic regression including demographic as well as clinical data as covariates. Two hundred fifty-eight patients were enrolled. One hundred fifty-eight patients received an antibiotic (mean duration 6.2 (±2.7) days (median 4; IQR: 4–7)). Presence of seizures and C-reactive protein values as predictors for antibiotic prescription (OR for seizures: 12.17; 95% CI: 1.42–103.91; p = 0.022; OR for CrP: 1.03; 95% CI: 1.01–1.04; p = 0.001). Seventy-four patients received intravenous antibiotics (74/156, 47.4%). Risk factors for intravenous antibiotic were the presence of decay (OR: 3.74; 95% CI: 1.25–11.71; p = 0.018), CrP values (OR: 1.02; 95% CI: 1.00–1.03; p = 0.001), and presence of seizures (OR: 16.34; 95% CI: 2.65–100.83; p = 0.003). Duration of intravenous antibiotics correlated with the presence of seizures (Coeff: 1.6; 95% CI: 0.41–2.89; p = 0.009) even when adjusted for CrP values. Conclusion: The clinical presentation of adenovirus infection in children is non-specific, leading to frequent antibiotic prescription despite bacterial co-infections was rare. Higher CrP values and presenting with seizures are significantly associated with a higher risk of receiving antibiotics. Rapid microbiological tests and newer biomarkers can help clinicians to improve antibiotic prescription in this cohort of children. What is Known:•Adenovirus infection is a common cause of fever and respiratory tract infections in children.•Children with adenovirus infections frequently receive antibiotics, but determinants of this practice are poorly established. What is New:• Higher C-reactive protein values and presenting with seizures are significantly associated with antibiotic prescription. • Since the beginning of COVID-19 and implementation of rapid diagnostics, less children with adenovirus infection received antibiotics.

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Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

Cited by 6 publications

References 24 publications

Changes in clinical, demographic, and outcome patterns of children hospitalized with non‐SARS‐CoV‐2 viral low respiratory tract infections before and during the COVID pandemic in Rome, Italy

Changes in clinical, demographic, and outcome patterns of children hospitalized with non‐SARS‐CoV‐2 viral low respiratory tract infections before and during the COVID pandemic in Rome, Italy

Distribution of Viral Respiratory Infections during the COVID-19 Pandemic Using the FilmArray Respiratory Panel

Determinants of antibiotic prescription in children with adenovirus respiratory tract infections

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