Comparative Evaluation of 29 Commercial <i><scp>H</scp>elicobacter pylori</i> Serological Kits

Burucoa, Christophe; Delchier, Jean‐Charles; Courillon-Mallet, Anne; Korwin, Jean-Dominique de; Mégraud, Françis; Zerbib, Frank; Raymond, Josette; Fauchère, Jean-Louis

doi:10.1111/hel.12030

Cited by 103 publications

(90 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Burucoa et al compared 29 commercial serological tests kits and reported sensitivities 55.6%-100%, specificities 59.6%-97.9%, positive predictive values 69.8%-100% and negative predictive value 68.3%-100% [34]. When culture was used as a gold standard, the sensitivities and the specificities of serological tests were 80-95% and 80-95% respectively [15] and with histology as a gold standard the sensitivities were ranging from 76% to 84% and the specificities were ranging from 79% to 90% [35].The overall performance of serology against predefined gold standard in our study was not satisfactory.…”

Section: Discussionmentioning

confidence: 99%

Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

Aftab

Yamaoka

Ahmed

et al. 2018

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Helicobacter pylori infection is associated with gastritis, peptic ulcer, and gastric cancer. We conducted a cross-sectional study to compare five diagnostic tests for H. pylori infection and studied the epidemiology of the infection in Bangladesh. Methodology: Bangladeshi patients with dyspeptic symptoms referred for endoscopic examination were enrolled in this study. Each patient underwent upper endoscopic examination and four gastric biopsy specimens were taken. We used 5 tests for the diagnosis of H. pylori; culture, histology confirmed by immunohistochemistry, rapid urease test (RUT), urinary and serological test. Demographic and environmental variables were collected. Results: A total of 133 patients participated in the study, 61 males and mean age 37.3 ± 12.3 years. We used the culture and/or histology results as the gold standard to estimate the sensitivity, specificity, positive and negative predictive values for the studied diagnostic tests. RUT, culture and histology had high sensitivity and specificity with moderate positive and negative likelihood ratio, whereas urine test and serology showed a good sensitivity and specificity but poor likelihood ratio. The overall prevalence of H. pylori among study subjects was 47% with no difference between gender and age groups. Conclusions: The invasive tests showed better performance than noninvasive tests among Bangladeshi population. The overall prevalence of H. pylori was less than the previously reported in the region with no difference among all age groups.

show abstract

Section: Discussionmentioning

confidence: 99%

Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

Aftab

Yamaoka

Ahmed

et al. 2018

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…Therefore, we aimed to validate and compare the diagnostic accuracy of Korean and Western serological assays for H. pylori detection in Korean adults. estimated that the sensitivity (or specificity) of the evaluated kits is 90 % (P50.9) with a 95 % confidence interval (Burucoa et al, 2013 Subjects. Korean adults who visited our centre between January and June of 2014 for the evaluation of H. pylori infection using the urea breath test (UBT) were included in this prospective study.…”

Section: Introductionmentioning

confidence: 99%

“…The serum anti-H. pylori immunoglobulin G (IgG) antibody test, which is both simple and inexpensive, is one of these tests (Burucoa et al, 2013;Faigel et al, 2000). Although it cannot distinguish between active and recently treated infections, serology is more accurate than biopsy in endemic areas of H. pylori infection and gastric cancer.…”

Section: Introductionmentioning

confidence: 99%

Validation of western Helicobacter pylori IgG antibody assays in Korean adults

Lee

Moon

Hur

et al. 2015

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

“…Serology is one of the most widely employed methods for screening larger populations, and several test systems have been developed. Most tests are based on enzyme-linked immunosorbent assay (ELISA) formats using H. pylori lysates or purified antigens, like CagA and VacA (21,22). They are cheap and easy to perform but either cannot discriminate between type I and type II strains or suffer from a lack of sensitivity, which could be due to the type or number of antigens used.…”

mentioning

confidence: 99%

A Novel Line Immunoassay Based on Recombinant Virulence Factors Enables Highly Specific and Sensitive Serologic Diagnosis of Helicobacter pylori Infection

Formichella

Romberg

Bolz

et al. 2013

Clin Vaccine Immunol

View full text Add to dashboard Cite

g Helicobacter pylori colonizes half of the world's population, and infection can lead to ulcers, gastric cancer, and mucosa-associated lymphoid tissue (MALT) lymphoma. Serology is the only test applicable for large-scale, population-based screening, but current tests are hampered by a lack of sensitivity and/or specificity. Also, no serologic test allows the differentiation of type I and type II strains, which is important for predicting the clinical outcome. H. pylori virulence factors have been associated with disease, but direct assessment of virulence factors requires invasive methods to obtain gastric biopsy specimens. Our work aimed at the development of a highly sensitive and specific, noninvasive serologic test to detect immune responses to important H. pylori virulence factors. This line immunoassay system (recomLine) is based on recombinant proteins. For this assay, six highly immunogenic virulence factors (CagA, VacA, GroEL, gGT, HcpC, and UreA) were expressed in Escherichia coli, purified, and immobilized to nitrocellulose membranes to detect serological immune responses in patient's sera. For the validation of the line assay, a cohort of 500 patients was screened, of which 290 (58.0%) were H. pylori negative and 210 (42.0%) were positive by histology. The assay showed sensitivity and specificity of 97.6% and 96.2%, respectively, compared to histology. In direct comparison to lysate blotting and enzyme-linked immunosorbent assay (ELISA), the recomLine assay had increased discriminatory power. For the assessment of individual risk for gastrointestinal disease, the test must be validated in a larger and defined patient cohort. Taking the data together, the recomLine assay provides a valuable tool for the diagnosis of H. pylori infection.

show abstract

Comparative Evaluation of 29 Commercial Helicobacter pylori Serological Kits

Abstract: These data should help the users to choose the kit the most appropriate to their goals.

Cited by 103 publications

References 31 publications

Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

Validation of diagnostic tests and epidemiology of Helicobacter pylori infection in Bangladesh

Validation of western Helicobacter pylori IgG antibody assays in Korean adults

A Novel Line Immunoassay Based on Recombinant Virulence Factors Enables Highly Specific and Sensitive Serologic Diagnosis of Helicobacter pylori Infection

Contact Info

Product

Resources

About