Comparative Estimates of Bacterial Affinities and Adsorption Sites on Hydroxyapatite Surfaces

Clark, William; Bammann, Lili Luschke; Gibbons, R. J.

doi:10.1128/iai.19.3.846-853.1978

Cited by 310 publications

(192 citation statements)

References 35 publications

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“…Previous research has demonstrated the ability of A. viseosusT\'^\ to adhere to saliva-coated hydroxyapatite beads (4,14,18). In the present study, we used hydroxyapatite disks because the bubble activity and streaming effects of the acoustic energy on the fiuid made use of beads impractical.…”

Section: Discussionmentioning

confidence: 97%

Fimbria damage and removal of adherent bacteria after exposure to acoustic energy

McInnes

Engel

Martin

1993

Oral Microbiology and Immunology

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The physical effects of low-frequency acoustic energy on Actinomyces viscosus were studied with electron microscopy to explore both acoustically induced damage to fimbriae on the surface of these bacteria and acoustic removal of bacteria from saliva-treated hydroxyapatite disks. A bacterial suspension was exposed to acoustic energy from a laboratory acoustic generator (50 kPa, 200 Hz) and from a new electronic toothbrush, the Sonicare. The exposed bacteria were examined with electron microscopy after negative staining. A decrease in both the percentage of bacterial surface covered with fimbriae and the fimbria length was observed after acoustic exposure. To study the acoustic effects on adherent bacteria, A. viscosus bound to hydroxyapatite disks were exposed to acoustic energy and examined with scanning electron microscopy. Quantitative evaluation of the micrographs for the number of bacteria present after exposure revealed that acoustic energy removed both bacteria adherent to the hydroxyapatite surface and adherent to each other. The results support the concept that an electronic toothbrush employing low-frequency acoustic energy may help prevent and control periodontal diseases by altering bacterial adherence.

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Section: Discussionmentioning

confidence: 97%

Fimbria damage and removal of adherent bacteria after exposure to acoustic energy

McInnes

Engel

Martin

1993

Oral Microbiology and Immunology

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“…In order to examine the effect of IgAl protease on the adherence-inhibiting activity of SIgA antibody, the hydroxyapatite (HA) adherence assay described by Clark et al (2) and modified by Kilian et al (12) and others (10,21) was employed. Aliquots of 40 mg of HA beads (BDH Chemicals, Ltd., Poole, England) were placed in 1.8 ml polypropylene microcentrifuge tubes and washed once with buffered KC1 to remove "fines" and allowed to equilibrate in 1.6 ml of buffered KC1 overnight.…”

mentioning

confidence: 99%

Effect of IgA1 Protease on the Ability of Secretory IgA1 Antibodies to Inhibit the Adherence of Streptococcus mutans

Tyler

Cole

1998

Microbiology and Immunology

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Secretory IgA (SIgA) is the principal immunoglobulin isotype present in the mucosal secretions of humans. SIgA is thought to play a major role in host defense at these surfaces by inhibiting the colonization of potentially pathogenic microorganisms. A number of bacteria that are mucosal pathogens of humans produce a protease that specifically cleaves the IgA1 subclass of humans and great apes at the hinge region to produce Fab and Fc fragments. In order to study the effect of IgA1 protease on the ability of SIgA1 antibodies to inhibit bacterial adherence, an in vitro assay that quantifies the adsorption of radiolabeled Streptococcus mutans to hydroxyapatite (HA) beads was employed. High titer S. mutans-specific SIgA1 and SIgA2 antibodies were induced in chimpanzee milk for use in the assay. Faba1 fragments had significantly reduced ability to inhibit adherence of S. mutans to saliva-coated HA compared to intact SIgA1 or SIgA2 anti-S. mutans antibodies. These data support the potential importance of IgA1 proteases as an ecological determinant in the oral cavity and their role as a determinant of pathogenesis of pathogenic bacteria whose portal of entry is the mucosal surface.

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“…Hydroxyapatite adsorption assay A modified version of the hydroxyapatite-bacterial adsorption assay, which has been described previously (11), was used to compare the in vitro adsorption affinities, of the Actinomyces strains used in these experiments. Briefly, Actinomyces strains were cultured in Tryptic Soy Broth supplemented with 10 |iCi of [^H] thymidine per ml (Amersham, Arlington Heights, IL).…”

Section: Eimbrial-mediated Colonization By a Naeslundii 261mentioning

confidence: 99%

“…Whole human and BALB/c mouse saliva were collected and stored until used as previously described (11,16). Hydroxyapatite preparations treated with human saliva or BALB/c mouse saliva were prepared by incubating 5 mg of washed spheroidal hydroxyapatite beads (BDH Biochemicals Ltd., Poole, UK) in 200 \i\ of whole human or mouse saliva for 1 h as previously described (16).…”

Section: Eimbrial-mediated Colonization By a Naeslundii 261mentioning

confidence: 99%

Fimbrial‐mediated colonization of murine teeth by Actinomyces naeslundii

Beem¹,

Hurley²,

Nesbitt³

et al. 1996

Oral Microbiology and Immunology

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Groups of mice fed diets high in sucrose or glucose were orally inoculated with 10(10), 10(9) or 10(8) colony-forming units of one of the following Actinomyces naeslundii strains possessing the type 1 (T1+) and/or the type 2 (T2+) fimbriae: T14VJ1 (T1+, T2+), 5519 (T1+), 5951 (T2+), and 147 (non-fimbriated). Ninety-six hours after inoculation their upper jaws were cultured to look at the implantation of each of these strains on the teeth. In mice fed a sucrose diet, regardless of the presence or absence of fimbriae, each bacterial strain colonized 100% of the mice at the highest inoculation doses of the infecting organism. But at a dose of 10(8), T14V-J1 was the only strain which colonized 100% (12/12) of the mice, 5519 colonized 10/11, 5951 colonized 9/11 and 147 colonized 7/11. These differences were not statistically significant. When mice were fed a high-glucose diet, 100% infection was achieved with strains T14V-J1, 5519 and 5951 only at the highest dose of 10(10) colony-forming units. Strain 147 colonized in 8/9 of the mice at that dosage. At lower dosages, no bacterial strain implanted in 100% of the mice. In the glucose experiment at a dose of 10(8), strains expressing the T1 fimbriae implanted significantly better than strains without the T1 fimbriae. At a dose of 10(9) colony-forming units, the parent strain T14V-J1 implanted significantly better than strains without the T1 fimbriae. Similarly, strain 5519 (T1+) implanted significantly better than 5951 and implanted better than 147, although the difference was not significant. These results suggest that while the presence of the T1 and T2 fimbriae may confer some advantage in the establishment of these organisms in vivo, even the strains without fimbriae were able to colonize. Strains T14VJ1 and 5519 were found to bind well to hydroxyapatite treated with mouse saliva, while strains 5951 and 147 did not. Only T2 fimbriated strains T14V-J1 and 5951 exhibited a lactose-reversible coaggreation with indigenous strains of enterococci that may contribute to the elevated levels of colonization of strain 5951 in vivo.

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Comparative Estimates of Bacterial Affinities and Adsorption Sites on Hydroxyapatite Surfaces

Cited by 310 publications

References 35 publications

Fimbria damage and removal of adherent bacteria after exposure to acoustic energy

Fimbria damage and removal of adherent bacteria after exposure to acoustic energy

Effect of IgA1 Protease on the Ability of Secretory IgA1 Antibodies to Inhibit the Adherence of Streptococcus mutans

Fimbrial‐mediated colonization of murine teeth by Actinomyces naeslundii

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