Comparative efficacy of different in vitro cultivation media for Trypanosoma evansi isolated from different mammalian hosts inhabiting different geographical areas of India

Abstract

The present study was undertaken to establish an optimal medium for primary culture initiation and maintenance of T. evansi isolated from different mammalian hosts of diverse geographical regions of India viz. donkey/1 (Hardoi, Uttar Pradesh), donkey/2 (Junagarh, Gujarat), pony/1 (Hisar, Haryana), camel/1 (Bikaner, Rajasthan) which represented isolates 1, 2, 3 and 4, respectively. Primary cultures were initiated with all four isolates in five different in vitro cultivation media with seeding density of 1 9 10 6 trypanosomes/ml. The parasites of all four isolates could remain viable only for 48 h in medium E (Alsever's solution) and for 72 h in medium A, C and D. Parasites reached to a maximum density (2.5-3.75 9 10 6 /ml) within 24 h and thereafter, a sharp decline (0.5-0.75 9 10 6 /ml) in the next 72 h was observed in 1, 2 and 3 isolates cultured in medium B. In isolate 4, parasite counts got more than doubled in 24 h and then decreased gradually up to sixth day post initiation of cultivation which thereafter increased gradually up to 34 days and a constant parasite number of 10 5 /ml could be achieved for 90 days in medium B. During this prolonged culture the trypanosomes retained their long slender morphology and infectivity to mice.

In vitro anti-trypanosomal effect of ivermectin on Trypanosoma evansi by targeting multiple metabolic pathways

“…evansi ) in Swiss albino mice (6–8 weeks old, female) obtained from Disease Free Small Animal House, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India, as described by Baltz et al ( 1985 ). At peak parasitemia (10 8 trypanosomes/mL), 4–5 days post-infection, trypanosomes were purified from mice blood as described by Kumar et al ( 2015 ). Prior approval for animal experimentation was taken from Institutional Animal Ethics Committee (IAEC) of ICAR-NRCE, Hisar [Regn No.…”

Molecular Characterization of Trypanosoma evansi Mevalonate Kinase (TeMVK)

“…Noteworthy, no effective protocol to maintain T. evansi isolates in axenic culture is available, which limits the execution and reproducibility of in vitro studies. Although many attempts have been made to cultivate different T. evansi isolates in vitro (Hirumi et al, 1997 ; Kumar et al, 2015 ; Birhanu et al, 2016 ), the parasite viability declines within few days and substantial molecular changes are introduced (e.g., complete loss of the kinetoplast). Alternatively, parasite propagation is achieved via experimentally infected animals (Hirumi et al, 1997 ).…”