Comparative efficacy of a hydroxyapatite and a fluoride toothpaste for prevention and remineralization of dental caries in children

Amaechi, Bennett T.; Azees, Parveez Ahamed Abdul; Alshareif, Dina O.; Shehata, Marina A.; Lima, Patrícia P.; Abdollahi, Azadeh; Kalkhorani, Parisa Samadi; Evans, Veronica

doi:10.1038/s41405-019-0026-8

Cited by 101 publications

(140 citation statements)

References 50 publications

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“…They explained the discrepancy to the findings from Huang et al by different components in their remineralization/demineralization buffers (mainly proteins) which may block the enamel surface to some extent [36]. In a recent in situ-study, Amaechi et al found remineralization of artificially produced carious lesions on enamel blocks that were worn in situ on intra-oral appliances after use of a toothpaste containing 10% HAP [39].…”

Section: Discussionmentioning

confidence: 91%

Ca2+ release and buffering effects of synthetic hydroxyapatite following bacterial acid challenge

et al. 2020

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Background: Synthetic particulate hydroxyapatite (HAP; Ca 5 (PO 4 ) 3 (OH)) is used as ingredient in oral care products but its effects on cariogenic biofilms are not clear yet. The primary mode of action of HAP may be acting as a calcium phosphate reservoir when deposited in oral biofilms and release Ca 2+ and (hydrogen) phosphate ions upon bacterial acid challenge. The aim of this in vitro study was to test this hypothesis by investigating release of Ca 2+ ions and potential buffering effects from HAP upon bacterial acid challenge in planktonic cultures and biofilms of Streptococcus mutans. Methods: Planktonic cultures of S. mutans were grown in BHI broth with 1% sucrose or with additional 5% HAP or 5% silica for up to 48 h. Separately, biofilms of S. mutans were grown in BHI for 72 h in total. After 24 h of this biofilm culture, either BHI alone or BHI with additional 0.5% HAP or 0.5% silica was added. After 48 h, BHI with 1% sucrose was added to allow bacterial acid formation. Ca 2+ release was determined colorimetrically and pH measurements were performed using a pH electrode. For statistical analysis, non-parametrical procedures were applied (n ≥ 10; Mann-Whitney U test; α = 0.05).Results: Relevant release of Ca 2+ was only evident in planktonic cultures or biofilms with HAP but not in both other groups (p ≤ 0.001). In suspended biofilms with HAP, median pH was 4.77 after 72 h and about 0.5 pH units higher as compared to both other groups (4.28 or 4.32, respectively; p ≤ 0.001).Conclusions: Under the tested conditions, synthetic HAP releases Ca 2+ ions upon bacterial acid challenge and may also show some buffering capacity but further studies are needed to investigate whether the concentrations tested here can also be reached clinically in dental biofilms.

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Section: Discussionmentioning

confidence: 91%

Ca2+ release and buffering effects of synthetic hydroxyapatite following bacterial acid challenge

et al. 2020

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“…33 In contrast, other in vitro and in situ studies have shown a remineralizing effect of HAP-containing toothpastes on enamel and/or dentin surfaces. Recent studies were published by Tschoppe et al 27 (in vitro study), Najibfard et al, 26 and Amaechi et al 28 (the latter are in situ studies).…”

Section: Resultsmentioning

confidence: 99%

“…Thus, a positive influence on the remineralization/demineralization process is very likely. 20,[26][27][28]…”

Section: Resultsmentioning

confidence: 99%

“…25 Thus, increasing calcium and phosphate levels at the tooth surface and/or in plaque by using calcium phosphate-based oral care products, is a promising preventive strategy to influence the demineralization/remineralization process positively to remineralization. 20,[26][27][28] Therefore, the aim of this in vivo study was to analyze the 3-day effects of a newly developed hydroxyapatite-based oral care gel on the calcium and phosphorus levels within the dental plaque of children. To the best of the authors' knowledge, this is the first study analyzing the effects of HAP in dental plaque under in vivo conditions.…”

Section: Introductionmentioning

confidence: 99%

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In Vivo Effects of a Hydroxyapatite-Based Oral Care Gel on the Calcium and Phosphorus Levels of Dental Plaque

Sudradjat¹,

Meyer

Loza

et al. 2020

Eur J Dent

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Objectives Particulate hydroxyapatite (HAP; Ca5(PO4)3(OH)) is a biomimetic oral care ingredient. One mode of action in caries-susceptible individuals may be the adhesion/incorporation of HAP into dental plaque. Therefore, the aim of this in vivo study was to analyze the 3-day effects of a newly developed hydroxyapatite-based oral care gel on the calcium and phosphorus levels within the dental plaque of children. Materials and Methods This study was conducted in Kebon Padangan at Bali in Indonesia. Thirty-four children (mean age, 8.9 years; mean DMF-T [decayed, missing, and filled teeth; permanent teeth], 0.6; mean dmft-t [primary teeth] 4.5) were included in the study. The gel was applied thrice for 3 days by an experienced dentist. Dental plaque was collected at baseline and after the study. Levels of calcium and phosphorus of plaque samples were analyzed by energy-dispersive X-ray spectroscopy. Statistical Analysis Medians for both calcium and phosphorus levels were calculated (baseline and 3-day application of HAP-gel). Results The calcium level increased after 3 days of application of the HAP-gel from 0.25 wt% (median) to 0.40 wt% (median), while the phosphorus level increased from 1.17 wt% (median) to 1.41 wt% (median). However, variations in both calcium and phosphorus levels measured in the pooled dental plaque samples were high. Conclusion Within the limitations of the study, the 3-day application of the oral HAP-gel in children increased the median of both calcium and phosphorus levels in plaque. Consequently, a positive influence on the remineralization/demineralization process is very likely.

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“…One of the recently proposed whitening agents is hydroxyapatite (HAP), which is the main component of dental hard tissues and bone and can be artificially synthesized [5]. HAP is used in dental treatments, such as for preventing enamel caries, restoring initial cavities, nano-hydroxyapatite-containing glass ionomer cements, and removing dental plaque in addition to hydroxyapatite-containing tooth pastes [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Adhesion and whitening effects of P11-4 self-assembling peptide and HAP suspension on bovine enamel

2020

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Objectives This study evaluated the adhesion and whitening effects of a combination of P11-4 self-assembling peptide and hydroxyapatite (peptide-HAP) on bovine enamel. Methods Forty-six caries-free bovine teeth were selected, and 40 teeth were randomly allocated to one of five groups (n = 8). First, the effects of application frequency, exposure time, and storage in saliva on the whitening effects of an experimental low-concentrated peptide-HAP suspension (0.5 wt% HAP; Curodont, Credentis) were evaluated and compared with a commercial bleaching agent (VivaStyle Paint on Plus, VS, Ivoclar Vivadent). Tooth color was measured using a spectrophotometer (Gretag MacBeth), and color changes ΔE were statistically analyzed. Second, the effects of peptide-HAP concentration (low versus high: 6.25% HAP; Curodont Protect), and its interactions with saliva and postapplication restaining, were investigated. Third, enamel surfaces (n = 2) were treated with low concentration peptide-HAP and high-concentration peptide-HAP in polymeric and monomeric forms (Curodont Protect & Curodont Repair, Credentis) and analyzed by SEM. Results The ΔE of the low-concentration peptide-HAP suspension did not differ from that of VS. Application frequency, exposure time, and storage in saliva did not have any significant impact on whitening efficacy of the peptide-HAP suspension. Increasing the concentration of the suspension did not promote overall ΔE. SEM observations confirmed the presence of the newly generated peptide and HAP on the enamel surface. Conclusions The peptide-HAP suspension is a mild tooth whitener, and the adhesion of peptide-HAP to enamel is concentration dependent. Clinical relevance This peptide-HAP suspension is effective in offsetting discoloration caused by restaining after treatment.

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Comparative efficacy of a hydroxyapatite and a fluoride toothpaste for prevention and remineralization of dental caries in children

Cited by 101 publications

References 50 publications

Ca2+ release and buffering effects of synthetic hydroxyapatite following bacterial acid challenge

Ca2+ release and buffering effects of synthetic hydroxyapatite following bacterial acid challenge

In Vivo Effects of a Hydroxyapatite-Based Oral Care Gel on the Calcium and Phosphorus Levels of Dental Plaque

Adhesion and whitening effects of P11-4 self-assembling peptide and HAP suspension on bovine enamel

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