Comparative effects of two different artificial body fluids on <I>Candida albicans </I>adhesion to soft lining materials

Abstract

This study investigated the C. albicans adhesion to cold-and heat-polymerized soft lining materials that were initially incubated in two different artificial body fluids, namely saliva and nasal secretion, and examined the surface roughness the materials (cold and heat polymerized soft liner) tested in vitro. Cold (Visco Gel) and heat-polymerized (Molloplast B) soft liner specimens (N=32, n=8 per group) (10×10×1.5 mm) were randomly produced to express the relationship between surface roughness and contamination, and influence of body fluids, and incubated in 1.5 ml contaminated solutions for 2 h. After fixation, all of materials were evaluated under optical microscope (×400) and SEM. Surface roughness measurements were examined with profilometre for each material. Data were analyzed using two-way ANOVA, Tukey's HSD and Dunnett T3 tests (α=0.05). Material type (p<0.05) and contamination media (p<0.05) showed a significant influence on the C. albicans adherence. The surface roughness of cold polymerized soft liner (Visco Gel) was significantly higher than heat-polymerized soft liner (Molloplast B) (p<0.05).

Farklı yumuşak astar materyallerinin Candida albicans biyofilm formasyonu açısından değerlendirilmesi

“…16,21 Farklı içerikli ısı ve oda ısı-sında polimerize olabilen silikon esaslı yumuşak astar materyallerinin farklı yüzey özelliklerinden dolayı C. albicans adezyonları ve sonrasında gelişen biyofilm formasyonları açısından aralarında farklılık olabileceği belirtilmiştir. 22 Ön-ceki çalışmaların çoğunda C. albicans bağlanması klinik durumu yansıtmayan düz yüzeyli yumuşak astar materyalleri üzerinde değerlendirilmiştir 12,16,23,24 Bu çalışmanın amacı, düz ve pürüzlü yüzeylerin silikon esaslı farklı yumuşak astar materyallerde C. albicans biyofilm oluşturma etkisini karşılaştırmak ve aynı zamanda bu durumun materyal hidrofobisitesi ile ilişkisini incelemektir.…”

Comparison of the Effect of Nystatin and Titanium Dioxide Addition on Candida Colonization in Soft Lining Material

“…12 Soft denture liners, namely soft polymers, can be applied to the mucosal or fitting area of the dentures. Soft liners diminish occlusal forces 4 and traumatic effects of dentures on the mucosa. They may be used in patients with resorbed ridges, deep anatomic undercuts, bony protuberances, and sharp alveolar ridge crests.…”

“…C. albicans is a eukaryotic, opportunistic pathogen that can easily colonize silicone materials. 1,4 The ease of colonization by C. albicans is thought to be due to the nature of the material and the difficulty in mechanically and chemically cleaning the liner. 18 Mechanical cleaning is particularly difficult in patients with reduced motor function.…”

“…2 According to the literature, this condition occurs in between 11% and 67% of denture wearers. [3][4][5] Local factors associated with dentures are also connected with denture stomatitis, such as the presence of a biofilm, [6][7][8][9] local trauma caused by dentures, 10 xerostomia, 11 continuous use of dentures, 12 and alteration in the salivary pH. 12 Soft denture liners, namely soft polymers, can be applied to the mucosal or fitting area of the dentures.…”

“…12 They may also be used in cases where the oral mucosa displays decreased toleration to the load imposed by the denture and in those with congenital and acquired oral defects requiring obturation. 4,[13][14][15] The use of these soft lining materials results in a more uniform dispersion of stress in the mucosa-lining interface with the denture. 15 Both temporary and permanent silicone rubber and acrylic resin liners are available.…”