Comparative effects of four surfactants on growth, contraction and adhesion of cultured human fibroblasts

Benoît, J.; Cormier, Michel; Wépierre, J.

doi:10.1007/bf00141290

Cited by 15 publications

(10 citation statements)

References 20 publications

(23 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It should be noted that the phase and fluorescence images of the wells were captured by an automated microscope (Keyence, USA), and the number of cells was counted by the Keyence counting program. For further chemical fixation methods ( Benoit et al., 1988 ), live imaging dyes ( Lulevich et al., 2009 ) were not used thus avoiding significant effects on viable cell adhesion.…”

Section: Methodsmentioning

confidence: 99%

Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics

et al. 2018

View full text Add to dashboard Cite

SummaryCellular membrane fluidity is a critical modulator of cell adhesion and migration, prompting us to define the systematic landscape of lineage-specific cellular fluidity throughout differentiation. Here, we have unveiled membrane fluidity landscapes in various lineages ranging from human pluripotency to differentiated progeny: (1) membrane rigidification precedes the exit from pluripotency, (2) membrane composition modulates activin signaling transmission, and (3) signatures are relatively germ layer specific presumably due to unique lipid compositions. By modulating variable lineage-specific fluidity, we developed a label-free “adhesion sorting (AdSort)” method with simple cultural manipulation, effectively eliminating pluripotent stem cells and purifying target population as a result of the over 1,150 of screened conditions combining compounds and matrices. These results underscore the important role of tunable membrane fluidity in influencing stem cell maintenance and differentiation that can be translated into lineage-specific cell purification strategy.

show abstract

Section: Methodsmentioning

confidence: 99%

Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Triton X-100 (TX100) is one of the most widely used nonionic surfactants for lysing cells to extract protein and other cellular organelles or to permeabilize the living cell membrane for transfection (1)(2)(3). However, if large amounts are added or the cells are subject to prolonged exposure to TX100, the cells die (4)(5)(6)(7). This toxicity of TX100 molecules arises because of the disrupting action of its polar head group on the hydrogen bonding present within the cell's lipid bilayer, leading to the destruction of the compactness and integrity of the lipid membrane.…”

mentioning

confidence: 99%

“…Moreover, cholesterol or similar molecule-rich membrane domains are reported to be more tolerant toward detergents such as TX100 molecules in comparison to other parts of the lipid bilayer. (8)(9)(10)(11)(12)(13)(14).…”

mentioning

confidence: 99%

Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM)

Koley

Bard

2010

Proc. Natl. Acad. Sci. U.S.A.

339

249

View full text Add to dashboard Cite

Changes in HeLa cell morphology, membrane permeability, and viability caused by the presence of Triton X-100 (TX100), a nonionic surfactant, were studied by scanning electrochemical microscopy (SECM). No change in membrane permeability was found at concentrations of 0.15 mM or lower during an experimental period of 30 to 60 min. Permeability of the cell membrane to the otherwise impermeable, highly charged hydrophilic molecule ferrocyanide was seen starting at concentrations of TX100 of about 0.17 mM. This concentration level of TX100 did not affect cell viability. Based on a simulation model, the membrane permeability for ferrocyanide molecules passing though the live cell membrane was 6.5 ± 2.0 × 10 -6 m/s. Cells underwent irreversible permeabilization of the membrane and structural collapse when the TX100 concentration reached the critical micelle concentration (CMC), in the range of 0.19 to 0.20 mM. The impermeability of ferrocyanide molecules in the absence of surfactant was also used to determine the height and diameter of a single living cell with the aid of the approach curve and probe scan methods in SECM.

show abstract

“…The same results have been confirmed for all four compounds tested in this study, both in the case of keratinocytes and fibroblasts. The stimulating effect of low SLS concentrations (below 0.06 mg/mL) on the growth of fibroblasts was also confirmed by Benoit et al who pointed to the concentration-dependent cytotoxic effect of this agent [46]. High SLS toxicity to keratinocytes has been demonstrated by Bigliardi et al who showed toxic effects on keratinocytes above 3 µg/mg and a proproliferative effect at low concentrations of this anionic surfactant [47].…”

mentioning

confidence: 72%

Complexes of Ectoine with the Anionic Surfactants as Active Ingredients of Cleansing Cosmetics with Reduced Irritating Potential

2020

View full text Add to dashboard Cite

For many years, an increasing number of diagnosed atopy and skin problems have been observed. For people affected by the problem of atopy, the selection of skin care products, including cosmetics, is extremely important. Cleansing cosmetics, due to their ability to cause skin irritations and disturb the hydrolipidic barrier, can increase problems with atopic skin. New solutions to reduce the effects of these products on the skin are very important. In this work, the effect of ectoine on the properties of anionic surfactants was analyzed. Based on model systems, analysis of the effect of ectoine on the irritating effect of four anionic surfactants and their ability to solubilize model sebum was performed. Antioxidant activity was also evaluated, and cytotoxic studies were performed on cell cultures. It was shown that the addition of ectoine to the anionic surfactant solutions improves its safety of use. After introducing ectoine to the surfactant solution, a decrease of irritant potential (about 20%) and a decrease in the ability to solubilize of model sebum (about 10–20%) was noted. Addition of ectoine to surfactant solutions also reduced their cytotoxicity by up to 60%. The obtained results indicate that ectoine may be a modern ingredient that improves the safety of cleansing cosmetics.

show abstract

Comparative effects of four surfactants on growth, contraction and adhesion of cultured human fibroblasts

Cited by 15 publications

References 20 publications

Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics

Defining Lineage-Specific Membrane Fluidity Signatures that Regulate Adhesion Kinetics

Triton X-100 concentration effects on membrane permeability of a single HeLa cell by scanning electrochemical microscopy (SECM)

Complexes of Ectoine with the Anionic Surfactants as Active Ingredients of Cleansing Cosmetics with Reduced Irritating Potential

Contact Info

Product

Resources

About