Comparative effects of ethylene inhibitors on Agrobacterium-mediated transformation of drought-tolerant wild watermelon

Abstract

Ethylene (CH), a phytohormone that is produced in response to both abiotic and biotic stresses, is an important factor influencing the efficiency of Agrobacterium-mediated transformation. In this study, effects of various ethylene inhibitors on the efficiency of Agrobacterium-mediated genetic transformation in drought-tolerant wild watermelon was comparatively examined. Consequently, in comparison to the application of chemical inhibitors such as AgNO and aminoethoxyvinylglycine (AVG), lower ethylene level was observed when the infecting Agrobacterium contained a gene for 1-aminocyclopropane-carboxylic acid (ACC) deaminase (acdS), which cleaves ethylene precursor ACC into α-ketobutyrate and ammonia. GUS histochemical and spectrophotometric enzyme assays showed that acdS was more effective in enhancing gene transfer than the chemical ethylene inhibitors. Efficiency of transgenic shoots formation was higher in acdS- and AVG-treated explants. These observations demonstrated that controlling the ethylene level during co-cultivation and shoot formation, particularly using the acdS-harboring Agrobacterium, is advantageous for enhancing the transformation efficiency in this plant.

Effects of cobalt ions and cobalt nanoparticles on transient expression of gus gene in catharanthus roseus suspension cultures

“…Supporting the previous point of view, inhibition of ethylene biosynthesis using Aminoethoxyvinylglycine (AVG) was associated with improvements in the efficiency of Agrobacterium-mediated transformation in bottle gourd (Han et al, 2005), carrot (Shin et al, 2017), and melon (Malambane et al, 2018;Nonaka et al, 2008).…”

“…In response to pathogen attack, ethylene contributes to stress signaling and provoking defense arsenal including pathogenesis-related (PR) proteins and several defense enzymes (Liang et al, 2013;Müller & Munné-Bosch, 2015;Taranto et al, 2017). Therefore, several attempts to enhance transformation efficiency aimed to manipulate ethylene (Han et al, 2005;Malambane et al, 2018;Nonaka et al, 2008;Seong et al, 2005;Sgamma et al, 2015).…”

Super-Agrobacterium ver. 4: Improving the Transformation Frequencies and Genetic Engineering Possibilities for Crop Plants

“…A. tumefaciens with AcdS and GabT was expected to cause reduced ethylene and GABA content in plants. Indeed, the ethylene levels in the plant tissues during the transformation were reduced by the A. tumefaciens with AcdS activity (Nonaka et al, 2008a; Malambane et al, 2018). On the other hand, significant differences in GABA content during the co-cultivation were not observed between A. tumefaciens with GabT activity and the control (data not shown).…”

“…Indeed, A. tumefaciens GV2260 that had AcdS activity introduced into it, was efficacious in the suppression of ethylene evolution from plant tissues during co-cultivation and increasing T-DNA transfer [Nonaka et al, 2008a (Super- Agrobacterium ver.1); Ntui et al, 2010; Hao et al, 2010]. Moreover, Super- Agrobacterium ver.1 showed stronger inhibition of ethylene evolution and higher T-DNA transfer frequencies than chemical treatments in melon and wild water melon (Nonaka et al, 2008a; Malambane et al, 2018). For the further improvement of Super- Agrobacterium ver.1, a stronger promoter was used to drive acdS .…”

Bioprospecting Plant Growth Promoting Rhizobacteria for Enhancing the Biological Properties and Phytochemical Composition of Medicinally Important Crops

“…A divergent group of plant growth promoting rhizobacteria secretes ACC deaminase which splits ACC into ammonia and α-ketobutyrate and thus limits its conversion to ethylene. Bacteria synthesizing ACC deaminase belongs to genera Pseudomonas [97], Bacillus [98], Acinetobacter [99], Azospirillum [100], Achromobacter [101], Enterobacter [102], Burkholderia [103], Agrobacterium [104], Rhizobium [105], Serratia [106], etc.…”