Comparative effects of chlorhexidine and essential oils containing mouth rinse on stem cells cultured on a titanium surface

Abstract

Chlorhexidine (CHX) and Listerine (LIS), an essential oil compound, are the two commonly used adjunctive agents for mechanical debridement, for reducing the bacterial load in the treatment of peri-implant inflammation. However, antimicrobial agents have been reported to be cytotoxic to the alveolar bone cells and gingival epithelial cells. The present study was performed to examine the effects of antiseptics CHX and LIS, on the morphology and proliferation of stem cells. Stem cells derived from the buccal fat pad were grown on machined titanium discs. Each disc was immersed in CHX or LIS for 30 sec, 1.5 min or 4.5 min. Cell morphology was evaluated with a confocal laser microscope and the viability of the cells was quantitatively analyzed with the cell counting kit-8 (CCK-8). The untreated cells attached to the titanium discs demonstrated well-organized actin cytoskeletons. No marked alterations in the cytoskeletal organization were observed in any of the treated groups. The treatment with CHX and LIS of the titanium discs decreased the viability of the cells grown on the treated discs (P<0.05). The stem cells derived from the buccal fat pad were sensitive to CHX and LIS, and a reduction in cellular viability was observed when these agents were applied to the discs for 30 sec. Further studies are required to determine the optimal application time and concentration of this antimicrobial agent for maximizing the reduction of the bacterial load and minimizing the cytotoxicity to the surrounding cells.

A Preliminary Investigation on the Antimicrobial Activity of Listerine®, Its Components, and of Mixtures Thereof

“…Undiluted Listerine® induced near‐complete cell death 24 h after a 60‐s treatment of human gingival and periodontal ligament fibroblasts (Tsourounakis et al , ). Likewise, a 30‐s treatment of stem cells derived from a buccal fat pad and grown on machined titanium disks showed a reduction in cellular viability (Park et al , ). On A‐375 cells, M exerted in vitro cytotoxic effects, with an IC 50 value of 0.012 mM (the M concentration in Listerine® is 2.69 mM) (Kijpornyongpan et al , ).…”

Bacterial adhesion mechanisms on dental implant surfaces and the influencing factors

“…Essential oils were found to decrease biofilm activity and biomass [134,135]. It was also reported to be antifungal and inhibitory to the adherence of C. albicans onto dental implants and cover screws in vitro [135], with commented that the essential oils were capable of promoting penetration to the deep structure of biofilm and destroy more pathogenic resistant forms.…”

Listerine® Products: An Update on the Efficacy and Safety

“…This reduction in cellular viability was observed even after 30 s of exposure. Park et al (2014) suggested that further studies should attempt to ascertain more exactly the optimal therapeutic range (frequency, duration, and concentration) for these rinses. That might at least minimize the cytotoxicity to the surrounding cells without reducing the usefulness of the products.…”