Comparative effects of Bacillus subtilis and Bacillus licheniformis on live performance, blood metabolites and intestinal features in broiler inoculated with Salmonella infection during the finisher phase

Abudabos, Alaeldein M.; Aljumaah, Mashael R.; Alkhulaifi, Manal M.; Al-Abdullatif, Abdulaziz A.; Suliman, Gamal M.; Sulaiman, Ali R. Al

doi:10.1016/j.micpath.2019.103870

Cited by 29 publications

(32 citation statements)

References 11 publications

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“…Similarly, Bacillus subtilis has been used as a probiotic with multiple functions in poultry [ 141 ]. As a growth promoter, for example, Bacillus subtilis has been demonstrated to improve chickens’ growth performance [ 142 , 143 ]; regulate intestinal microstructure [ 144 ] and digestive enzymes [ 145 , 146 ]; synthesize and release antimicrobial and antibiotic compounds [ 147 , 148 , 149 , 150 ]; increase immunity against gut inflammation and infectious diseases [ 151 , 152 ]; and enhance neurochemical activities [ 153 , 154 ] via the bidirectional communication between the gut and brain, regulating stress responses under various rearing conditions [ 155 , 156 , 157 ].…”

Section: Probiotics and The Gut–microbiota–brain Axis In Broilersmentioning

confidence: 99%

Bacillus subtilis-Based Probiotic Improves Skeletal Health and Immunity in Broiler Chickens Exposed to Heat Stress

Jiang

Yan

et al. 2021

Animals

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The elevation of ambient temperature beyond the thermoneutral zone leads to heat stress, which is a growing health and welfare issue for homeothermic animals aiming to maintain relatively constant reproducibility and survivability. Particularly, global warming over the past decades has resulted in more hot days with more intense, frequent, and long-lasting heat waves, resulting in a global surge in animals suffering from heat stress. Heat stress causes pathophysiological changes in animals, increasing stress sensitivity and immunosuppression, consequently leading to increased intestinal permeability (leaky gut) and related neuroinflammation. Probiotics, as well as prebiotics and synbiotics, have been used to prevent or reduce stress-induced negative effects on physiological and behavioral homeostasis in humans and various animals. The current data indicate dietary supplementation with a Bacillus subtilis-based probiotic has similar functions in poultry. This review highlights the recent findings on the effects of the probiotic Bacillus subtilis on skeletal health of broiler chickens exposed to heat stress. It provides insights to aid in the development of practical strategies for improving health and performance in poultry.

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Section: Probiotics and The Gut–microbiota–brain Axis In Broilersmentioning

confidence: 99%

Bacillus subtilis-Based Probiotic Improves Skeletal Health and Immunity in Broiler Chickens Exposed to Heat Stress

Jiang

Yan

et al. 2021

Animals

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“…Moreover, B. subtilis is beneficial to the host health through modulation of microbiota composition and humoral immune responses ( Khan and Chousalkar, 2020 ; Kawarizadeh et al., 2021 ). Recently, several clinical and animal trials have demonstrated the protection of B. subtilis against Salmonella and other enteropathogenic bacteria in broilers through the load reduction in intestinal tracts, improvement of the growth performance, and immune responses ( Wu et al., 2018 ; Sokale et al., 2019 ; Abudabos et al., 2020 ). Nevertheless, the action modes of probiotic B. subtilis are in a strain-specific manner ( Freedman et al., 2021 ), which makes it necessary to investigate the potential protection against Salmonella infection in poultry farming.…”

Section: Introductionmentioning

confidence: 99%

Probiotic Bacillus subtilis LF11 Protects Intestinal Epithelium Against Salmonella Infection

Zhang

et al. 2022

Front. Cell. Infect. Microbiol.

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Enteric diseases caused by Salmonella are prevalent in poultry farming. With the forbiddance of antibiotics in feedstuff industry, Bacillus subtilis (B. subtilis) preparation as antibiotic alternatives against Salmonella infection has gained increasing attention recently. However, the protection modes of B. subtilis against Salmonella infection in broilers are strain-specific. In this study, probiotic B. subtilis LF11 significantly reduced diarrhea and mortality of broilers caused by Salmonella braenderup (S. braenderup) in spite of no inhibition effect on it in vitro. Here, the intestinal epithelial cells NCM460 were incubated to explore the protection of B. subtilis LF11 on intestinal epithelium against Salmonella. The results revealed that B. subtilis LF11 showed obvious exclusion activity with the decrease of adhesion and invasion of S. braenderup to NCM460 cells, accordingly with the increase of NCM460 cell survival compared with S. braenderup challenge alone. Meanwhile, RT-PCR and Western blot proved that the gene transcription and expression levels of four tight junction proteins in NCM 460 cells were upregulated, which was further confirmed by immunofluorescence observation. Besides, B. subtilis LF11 downregulated the gene transcription levels of the proinflammatory cytokines IL-6, IL-8, and TNF-α induced by S. braenderup H9812. ELISA analysis also verified that B. subtilis LF11 reduced the IL-8 production significantly. In general, B. subtilis LF11 has the ability to protect the intestinal epithelium against Salmonella infection by reducing the Salmonella adhesion and invasion, enhancing the intestinal barrier and attenuating the enterocyte inflammatory responses, and has the potential as probiotics to prevent enteric diseases in broilers.

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“…The use of spores or products fermented by B. licheniformis and/or B. subtilis has resulted in a benefit to the consumer. For example, they have been used in the poultry industry to promote the growth of chickens and to eliminate pathogenic infectious agents such as Salmonella [9,32]. In another more recent study, it was described that Bacillus could inhibit the growth and adhesion of enterotoxigenic E. coli (ETEC) [33].…”

Section: Discussionmentioning

confidence: 99%

“…B. licheniformis and B. subtilis are probiotics that have been used to prevent the growth of microorganisms considered pathogens such as E. coli or Salmonella [32,33]. The results obtained so far indicate that both probiotics induce the production of METs.…”

Section: Mets Induced By B Licheniformis Inhibit the Growth Of Staphylococcus Aureusmentioning

confidence: 99%

Bacillus licheniformis and Bacillus subtilis, Probiotics That Induce the Formation of Macrophage Extracellular Traps

et al. 2021

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Probiotics are considered living microorganisms that help preserve the health of the host who uses them. Bacillus are a genus of Gram-positive bacteria used as probiotics for animal and human consumption. They are currently distributed in various commercial forms. Two of the species used as probiotics are B. licheniformis and B. subtilis. Macrophages are central cells in the immune response, being fundamental in the elimination of microbial pathogens, for which they use various mechanisms, including the formation of extracellular traps (METs). There have been very few studies carried out on the participation of macrophages in response to the interaction of probiotics of the genus Bacillus with the host. In this work, we used macrophages from the J774A mouse cell line.1, and we found that they are susceptible to infection by the two Bacillus species. However, both species were eliminated as the infection progressed. Using confocal microscopy, we identified the formation of METs from the first hours of infection, which were characterized by the presence of myeloperoxidase (MPO) and citrullinated histone (Hit3Cit). Quantitative data on extracellular DNA release were also obtained; release was observed starting in the first hour of infection. The induction of METs by B. licheniformis caused a significant decrease in the colony-forming units (CFU) of Staphylococcus aureus. The induction of METS by bacteria of the Bacillus genus is a mechanism that participates in controlling the probiotic and potentially pathogenic bacteria such as S. aureus. The induction of METs to control pathogens may be a novel mechanism that could explain the beneficial effects of probiotics of the genus Bacillus.

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Comparative effects of Bacillus subtilis and Bacillus licheniformis on live performance, blood metabolites and intestinal features in broiler inoculated with Salmonella infection during the finisher phase

Cited by 29 publications

References 11 publications

Bacillus subtilis-Based Probiotic Improves Skeletal Health and Immunity in Broiler Chickens Exposed to Heat Stress

Bacillus subtilis-Based Probiotic Improves Skeletal Health and Immunity in Broiler Chickens Exposed to Heat Stress

Probiotic Bacillus subtilis LF11 Protects Intestinal Epithelium Against Salmonella Infection

Bacillus licheniformis and Bacillus subtilis, Probiotics That Induce the Formation of Macrophage Extracellular Traps

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