Comparative effects of addition of superoxide dismutase and reduced glutathione on cryopreservation of Sahiwal bull semen

Abstract

The present study aimed to investigate effects of superoxide dismutase (SOD) and reduced glutathione (GSH) on the quality of frozen-thawed semen of Sahiwal bulls. Semen was collected twice a week for 8 weeks by artificial vagina from six Sahiwal bulls, kept at the Semen Production Unit Qadirabad, Sahiwal-Pakistan. After gross and microscopic evaluation, qualifying semen ejaculates were divided into 10 equal aliquots and diluted in extenders enriched with no antioxidants (control); or supplemented with either SOD (50, 100 and 200 IU/mL), or GSH (0.5, 1 and 2 mM) or their combinations (50 IU/mL SOD and 0.5 mM GSH, 100 IU/mL SOD and 1 mM GSH and 200 IU/mL SOD and 2 mM GSH). Samples were then frozen and stored in liquid nitrogen at -196°C for 24 h. The following parameters were evaluated for semen quality: post-thawed sperm motility, viability, acrosome and membrane integrity. According to the results, sperm motility, viability, acrosome and membrane integrity were significantly (P<0.05) higher in samples treated either with 100 IU/mL of SOD; 1 mM and 2 mM of GSH or 50 IU/mL of SOD plus 0.5 mM of GSH. In conclusion, semen quality might be improved by supplementing semen extenders with 100 IU/mL of SOD; 0.5 and 1 mM of GSH and combination of 50 IU/mL and 0.5 mM of SOD and GSH, respectively.

Effect of Swim-Up and Glass Wool Techniques, With Adding Antioxidants to Tris Extender on Improving Post-Cryopreserved Some Semen Attributes of Low Semen Quality for Holstein Bulls

“…The L3 group illustrated nonsignificant differences with L1 group at all PC periods. These results were concurrent with (12,46) findings and disagreed with Murtaza (42) who found that sperm's cell motility percentage was decreased when added 200 IU SOD to Tris extender (45.00±0.408%) as compared with control group (Tris extender) in Sahiwal bull semen. Daghigh and Griveau (18,28) pointed out that SOD resulted in higher motility of epididymal bull sperm post cryopreservation, these results was disagreement with our result.…”