Comparative effectiveness of Pseudomonas and Serratia sp. containing ACC-deaminase for improving growth and yield of wheat (Triticum aestivum L.) under salt-stressed conditions

Abstract

Ethylene synthesis is accelerated in response to various environmental stresses like salinity. Ten rhizobacterial strains isolated from wheat rhizosphere taken from different salt affected areas were screened for growth promotion of wheat under axenic conditions at 1, 5, 10 and 15 dS m(-1). Three strains, i.e., Pseudomonas putida (N21), Pseudomonas aeruginosa (N39) and Serratia proteamaculans (M35) showing promising performance under axenic conditions were selected for a pot trial at 1.63 (original), 5, 10 and 15 dS m(-1). Results showed that inoculation was effective even in the presence of higher salinity levels. P. putida was the most efficient strain compared to the other strains and significantly increased the plant height, root length, grain yield, 100-grain weight and straw yield up to 52, 60, 76, 19 and 67%, respectively, over uninoculated control at 15 dS m(-1). Similarly, chlorophyll content and K(+)/Na(+) of leaves also increased by P. putida over control. It is highly likely that under salinity stress, 1-aminocyclopropane-1-carboxylic acid-deaminase activity of these microbial strains might have caused reduction in the synthesis of stress (salt)-induced inhibitory levels of ethylene. The results suggested that these strains could be employed for salinity tolerance in wheat; however, P. putida may have better prospects in stress alleviation/reduction.

The Role of Soil Beneficial Bacteria in Wheat Production: A Review

“…Similar outcome has been reported by Nia et al for Azospirillum strains on wheat plants [144]. Several PGPR of the genus Pseudomonas contain ACC-deaminase enzyme, and when inoculated into plant roots may sustain plant growth under salinity [125,142].…”

“…Wheat (Triticum aestivum L.) [142] Salinity Azospirillum sp Durum wheat (Triticum durum) [128] [207] Azorhizobium caulinodans Wheat N2 fixation Dry weight, nitrogen content [192] Azotobacter chroococcum Wheat Rhizospheric P solubilization, N2 fixation, IAA Seed emergence radicle and plumule length [114] Azotobacter sp.…”

“…The isolation of indigenous microorganisms from the stress-affected soils and screening on the basis of their stress tolerance and PGP traits may be useful in the rapid selection of efficient strains that could be used as bio-inoculants for stressed crops [139,140]. For several durum cultivars, PGPR efficacy in mitigating salt stress in tetraploid wheat is salt level and bacterial strainspecific [128,141,142]. There are some instances of ameliorating salt-stricken cereal crops by PGPR's.…”

Desiccation-Tolerant Rhizobacteria from Cholistan Desert, Pakistan, and Their Impact on Zea mays L.

“…broth, TSB, EPS medium, and Pharmamedia at various pH (6,7,8,9, and 10) and temperatures (25ºC, 30ºC, 37ºC, 42ºC, and 46ºC) -were tested for their ability to enhance EPS production by the select isolates. After 72 h of incubation, the maximum growth of all isolates was observed in Pharmamedia and maximum EPS yield was observed in TSB medium at optimum temperature of 37ºC and neutral pH of 7 (Fig.…”

Isolation and characterization of 1-aminocyclopropane-1-carboxylate (ACC) deaminase-containing plant growth-promoting rhizobacteria from carnation soil and roots

“…About half of arable lands in the world are saline (Jalili et al, 2009;Zahir et al, 2009). Yunnan province, located at the southwest part of China, is a semiarid region and the largest hub of carnation cultivation, in which most carnations are grown in the greenhouse.…”