Comparative Effectiveness of Bone Grafting Using Xenograft Freeze-Dried Cortical Bovine, Allograft Freeze-Dried Cortical New Zealand White Rabbit, Xenograft Hydroxyapatite Bovine, and Xenograft Demineralized Bone Matrix Bovine in Bone Defect of Femoral Diaphysis of White Rabbit: Experimental Study In Vivo

Abstract

Autogenous bone graft is gold standard in treating bone defects, but it might have difficulty in corporation and rejection reaction. This study is to compare the effectiveness among freeze-dried xenograft, freeze-dried allograft, hydroxyapatite xenograft, and demineralized bone matrix xenograft as bone graft to fill bone defect in femoral diaphysis of white rabbit. Thirty male New Zealand white rabbits were distributed into five groups. Bone defect was filled correspondingly with xenograft freeze-dried cortical bovine, allograft freeze-dried cortical New Zealand white rabbit, xenograft hydroxyapatite bovine, and xenograft demineralized bone matrix bovine. No graft was used in control group. VEGF, osteoblast, and woven bone were higher in allograft freeze-dried cortical New Zealand white rabbit (mean 5.6625 (p < 0.05)) and xenograft demineralized bone matrix bovine (mean 5.2475 (p < 0.05)) with calcification of woven bone was already seen in week 2 in the latter group. There was a decrease of woven bone (mean 4.685 (p < 0.05)) fibrous tissue (mean 41.07 (p < 0.05)) in xenograft demineralized bone matrix bovine. The Immunoglobulin-G was elevated in control and all study groups but not significantly (p = 0.07855). Bone healing process in xenograft demineralized bone matrix bovine is more effective than in xenograft hydroxyapatite bovine, allograft freeze-dried New Zealand white rabbit, xenograft freeze-dried cortical bovine, and control.

Kesembuhan Fraktur Tulang Femur Kelinci Pasca Implantasi Bahan Cangkok Demineralisasi Serbuk Tulang Sapi Bali

“…Pada kelompok perlakuan minggu ke-4 terlihat adanya neovaskularisasi, fibroblas, kartilago, tulang trabekula osteoblas dan osteosit (Gambar 4). Hal ini sesuai penelitian Mahyudin et al (2017), bahwa pada minggu ke-4 terlihat adanya jaringan fibrosa dan tulang fibrosa (woven bone), yang menandakan mulainya proses kalsifikasi. Salamanca et al (2018), juga berpendapat pada tahap ini memungkinkan untuk mengamati sel-sel inflamasi dan sebagian besar tulang fibrosa (woven bone) dikelilingi oleh osteoblas.…”

“…Pada minggu selanjutnya jaringan fibrosa dan tulang Volume 11 No. 2: 203-211 pISSN: 2085-2495eISSN: 2477eISSN: -2712 Agustus 2019 Online pada: http://ojs.unud.ac.id/index.php/buletinvet DOI: 10.24843/bulvet.2019.v11.i02.p13 fibrosa (woven bone) menurun dengan tulang fibrosa (woven bone) tipis yang sudah terkalsifikasi (Mahyudin et al, 2017). Pada kelompok perlakuan di minggu ke-6 terjadi peningkatan osteoblas, dan osteosit karena pertumbuhan tulang baru yang lebih intens pasca tulang tersebut diberikan bahan cangkok (Gambar 6) dibandingkan dengan kelompok kontrol dan pada semua kelompok perlakuan di minggu ke-4.…”

“…Hal tersebut akan mempercepat fagositosis oleh osteoklas, diikuti dengan peningkatan osteoblas yang akan mempercepat pembentukan tulang fibrosa (woven bone) dan degradasi jaringan fibrosa (Kinney et al, 2010). Pada penelitian Mahyudin et al (2017) dijelaskan bahwa sebelum penyembuhan tulang terjadi peningkatan osteoblas, osteoklas serta terbentuknya tulang fibrosa (woven bone), selanjutnya tulang fibrosa (woven bone) akan berkurang pada minggu berikutnya karena porses kalsifikasi. Proses pembentukan callus mulai terjadi dari minggu kedua dan terjadi secara maksimum pada minggu keenam bersamaan dengan kalsifikasi hingga penyembuhan terjadi.…”

<i>In Vitro</i> Comparative Study of Osteogenic Differentiation Ability between Adipose and Bone Marrow Mesenchymal Stem Cell Applied to Bovine Demineralized Bone Matrix

“…Considering this, bovine Demineralized Bone Matrix (DBM) which is a xenogenic bone graft product can be rated as the alternative bone graft substitues, because its osteoconductive and osteoinductive properties can serve as a scaffold to support stem cell proliferation and differentiation thus provide osteogenic capacity [9]. Besides its economically cost, bovine DBM has been shown to have higher bone healing promotion capacities than the other type of bone graft and has lower immunogenicity and rejection reactions to surrounding tissue [10]. This current study will test and compare the ability of bovine DBM as scaffold and growth factor provider to stimulate differentiation of bone marrow and adipose mesenchymal stem cells to enhance its function as a bone graft.…”

Comparative characteristics of the xenogenic synthetic biomimetic composite and spongy bone tissue for the goals of osteoplasty in dentistry

“…At present synthetic organic-mineral materials with qualitatively different properties are elaborated, investigated, and applied in order to replace various defects of the hard dental tissues [1][2][3][4]. The nature of these materials can be either purely synthetic: materials are obtained as a product of chemical relations or biogenic ones where samples represent a kind of the treatment of a biological object [5,6]. Both of the approaches are aimed at designing dental materials including those for oral surgery with the structure, composition and morphology that are closest to hard human tissues.…”