Comparative effect of ozone exposure and 5-aminosalicylic acid in oxazolon-induced colitis upon the indices of innate immunity

Abstract

Multiple effects of ozone are a prerequisite for its use in the complex therapy of inflammatory bowel diseases. Our aim was to analyze the effects of ozone and 5-aminosalicylic acid (5-ASA) in oxazolone-induced colitis (OIC) upon the innate immunity functions.
 OIC was modeled in 72 male Wistar rats weighing 24020 g by a two-stage application of oxazolone solution. Ozone at a dose of 0.05 mg/kg, as a part of ozone-oxygen mixture, was injected intraperitoneally every 24 hours. The laboratory indices were studied on the days 2, 4, 6. In blood samples, the number of leukocytes, differential leukocyte counts, absorptive capacity of blood neutrophils and their oxygen-dependent metabolism were studied. In homogenate of the colon mucosa, IL-17 concentration was measured.
 In OIC, on days 2, 4, and 6, the number of blood leukocytes was increased, with a predominance of lymphocytes, monocytes, neutrophils and their absorption capacity; the concentration of IL-17 increased in the areas of colonic damage. On days 2 and 4, the activity and intensity of the spontaneous NBT test is increased like as activity and intensity of induced NBT-test on days 2 and 6. Administration of ozone reduced the content of blood leukocytes, lymphocytes, neutrophils on days 2 and 6, like as their absorption capacity on days 2 and 4, along with a decrease in NCT-reducing ability on days 6, and decreased IL-17 concentration in the area of colonic damage on days 4 and 6. The effects of ozone administration in OIC, if compared with 5-ASA, are less pronounced on days 2 and 4, with respect to decreased number of monocytes, neutrophils in blood and their absorption capacity on days 2, like as IL-17 concentration in the area of colonic damage on days 4 and 6.