Comparative detection and recovery of Pseudomonas aeruginosa by membrane filtration and a Most Probable Number technique

Ngwa, G.A.; Schop, R.; Chow, J.; Lukić, L.; McKague, K.

doi:10.1016/j.mimet.2016.12.012

Cited by 5 publications

(6 citation statements)

References 11 publications

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“…It is known that P. aeruginosa can be found at locations near the runoff of treatment plants [30], resulting in contaminated waters that are frequently described as a vehicle for infections [16,31,32]. In the present study, there were mean counts of 135.8 CFU/100 mL of P. aeruginosa in the water collected near the STP runoff.…”

Section: Discussionmentioning

confidence: 45%

“…Opportunistic bacteria, such as Pseudomonas aeruginosa [13], might better serve to indicate the occurrence of some of the nonfaecal pathogens [12], especially in bathing waters [14]. Taking into account the results found in a study from Mariño and coworkers, where a positive correlation was found between skin infections and the presence of P. aeruginosa in seawater [14], this opportunistic bacteria [13] might better serve to indicate the occurrence of some nonfaecal pathogens in bathing waters [12].Being rod-shaped, gram-negative, facultative aerobic bacterium, P. aeruginosa has minimal survival requirements [15,16] and a remarkable adaptation ability towards a variety of environmental conditions, being able to thrive in soil, water, animal hosts, hospital settings, soap, and even distilled water [17][18][19][20]. P. aeruginosa, as the origin of an extensive variety of infections, is a main cause of illness in immunocompromised individuals [21].…”

mentioning

confidence: 98%

“…Being rod-shaped, gram-negative, facultative aerobic bacterium, P. aeruginosa has minimal survival requirements [15,16] and a remarkable adaptation ability towards a variety of environmental conditions, being able to thrive in soil, water, animal hosts, hospital settings, soap, and even distilled water [17][18][19][20]. P. aeruginosa, as the origin of an extensive variety of infections, is a main cause of illness in immunocompromised individuals [21].…”

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confidence: 99%

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Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

et al. 2019

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To estimate the quality of coastal waters, European Union Directive 2006/7/EC provides guidelines to assess levels of faecal bacteria, including Escherichia coli and intestinal enterococci. These microbiological criteria are based on studies that determine the risk of bathers having diseases caused by enteric bacteria, not necessarily measuring the potential danger associated with the presence of nonenteric pathogens. The association between the presence of faecal contaminant indicators and nonenteric pathogenic microorganisms has not been well defined yet. The purpose of this study is to establish a relationship between Pseudomonas aeruginosa and microbiological indicators of faecal contamination. Presence of microbiological contamination in the coastal waters near the sewage treatment plant (STP) of Peniche (Portugal) was confirmed (P. aeruginosa 135.8 Colony Forming Unit/100 mL, Escherichia coli 1100.1 Most Probable Number/100 mL, intestinal enterococci 2685.9 MPN/100 mL) with much lower levels in the areas located south of the STP, along the main water coastal current (beach 1: 0.7 CFU/100 mL, 16.5 MPN/100 mL, 100.5 MPN/100 mL; beach 2: 0.3 CFU/100 mL, 74.0 MPN/100 mL, 145.9 MPN/100 mL, respectively). Analysis of Pearson's correlation revealed a strong positive correlation between E. coli and P. aeruginosa, suggesting E. coli as an indicator of P. aeruginosa presence.Pathogens 2020, 9, 13 2 of 10 throat diseases [11]. These indicators were based on conclusions from epidemiological studies that implicate the enteric bacteria E. coli and enterococci in cases of gastroenteritis associated with swimming. However, the majority of recently reported cases of infectious diseases linked with coastal bathing waters are of nonenteric origin [12], creating a requirement for nonenteric indicator organisms that must be additionally evaluated. Opportunistic bacteria, such as Pseudomonas aeruginosa [13], might better serve to indicate the occurrence of some of the nonfaecal pathogens [12], especially in bathing waters [14]. Taking into account the results found in a study from Mariño and coworkers, where a positive correlation was found between skin infections and the presence of P. aeruginosa in seawater [14], this opportunistic bacteria [13] might better serve to indicate the occurrence of some nonfaecal pathogens in bathing waters [12].Being rod-shaped, gram-negative, facultative aerobic bacterium, P. aeruginosa has minimal survival requirements [15,16] and a remarkable adaptation ability towards a variety of environmental conditions, being able to thrive in soil, water, animal hosts, hospital settings, soap, and even distilled water [17][18][19][20]. P. aeruginosa, as the origin of an extensive variety of infections, is a main cause of illness in immunocompromised individuals [21]. Some examples are endocarditis, urinary tract infections, pneumonia, gastrointestinal infections, and meningitis, and it is also a leading cause of septicaemia [19]. P. aeruginosa is the pathogen mostly implicated in folliculitis and ear infec...

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Section: Discussionmentioning

confidence: 45%

mentioning

confidence: 98%

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confidence: 99%

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Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

et al. 2019

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“…2011), and 20% for Pseudalert (Ngwa et al . 2017). These false‐positive results are suspected to occur when non‐target bacteria, that cleave the required substrates, are present in the tested water samples (Sercu et al .…”

Section: Resultsmentioning

confidence: 99%

“…2011; Ngwa et al . 2017). Anecdotal reports from users of the IDEXX Legiolert and the Centers for Disease Control and Prevention (CDC) suggest that this cross‐reaction phenomenon may similarly pose a problem with the Legiolert method in engineered water systems.…”

Section: Introductionmentioning

confidence: 99%

Cross‐reactivity of the IDEXX Legiolert method with other Gram‐negative bacteria and waterborne pathogens leads to false‐positive assay results

Hirsh¹,

Baron²,

Mietzner³

et al. 2021

Letters Applied Microbiology

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Legionella species are the causative agent of Legionnaires’ disease, a potentially fatal bacterial pneumonia. New regulations and standards have prioritized the development of water safety plans to minimize the growth and spread of Legionella species in buildings. To determine the presence and type of Legionella in a water system, microbiological culturing is the gold standard method. However, recently new methodologies have been developed that claim to be sensitive and specific for Legionella at the genus or L. pneumophila at the species level. Published and anecdotal reports suggest that one of these newer culture‐based, enzyme‐substrate methods, the IDEXX Legiolert test, may exhibit false positivity with other microbes common to water sources. We experimentally evaluated the IDEXX Legiolert method using these other waterborne bacteria including Elizabethkingia meningoseptica, Pseudomonas aeruginosa, Proteus mirabilis and Serratia marcescens at real‐world environmental concentrations. We saw false‐positive results for the Legiolert test with several of these organisms, at sample concentrations as low as 60 CFU per ml. False‐positive Legionella results can trigger costly remediation and water‐use restrictions, that may be implemented while waiting for additional, confirmatory microbiological testing that could, in this case, yield no L. pneumophila.

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Examining the microbial composition of natural springs in Bhaderwah, Jammu and Kashmir, India

Gupta

Dutt

Sharma

et al. 2023

Environ Monit Assess

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Comparative detection and recovery of Pseudomonas aeruginosa by membrane filtration and a Most Probable Number technique

Cited by 5 publications

References 11 publications

Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

Faecal Indicator Bacteria and Pseudomonas aeruginosa in Marine Coastal Waters: Is there a Relationship?

Cross‐reactivity of the IDEXX Legiolert method with other Gram‐negative bacteria and waterborne pathogens leads to false‐positive assay results

Examining the microbial composition of natural springs in Bhaderwah, Jammu and Kashmir, India

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