Comparative Cytotoxicity of Selected Mikania species using Brine Shrimp Lethality Bioassay and Sulforhodamine B (SRB) Assay

Abstract

This study aims to assess the comparative cytotoxic activity of the ethanolic extract of Mikania cordata (MC), Mikania micrantha (MM) and Mikania scandens (MS) (Family: Asteraceae) using brine shrimp lethality bioassay and colorimetric sulforhodamine B assay method. In SRB assay, A549 human lung carcinoma, SK-Mel-2 skin melanoma and B16F1 mouse melanoma cell lines were used. In brine shrimp assay, the LC50 for MC, MM, MS and vincristine were found to be 29.04, 15.84, 32.35, and 1.2 μg/ml, respectively. The results indicate that all the Mikania species showed moderate lethality against nauplii. In SRB assay, 50% cell growth inhibition concentration (IC50) of MC, MM, MS and cisplatin against B16-F1 were 33, 15, 39 and 6.8 μg/ml, respectively. Similar data were observed for other cell lines indicating moderate cytotoxicity of the extracts. Among the species, M. micrantha showed relatively potent cytotoxic effect followed by M. cordata and M. scandens. Similar data were observed for brine shrimp lethality bioassay and the results suggest that M. micrantha possesses highest cytotoxic potentials among all the species.
 Bangladesh Pharmaceutical Journal 24(1): 11-16, 2021

GC-MS analysis and invitro cytotoxic activity of Ocimum basilicum (Lamiaceae) volatile oil and active fraction composed majorly of estragole

“…In vivo lethality in basic zoological species can be utilized as a useful monitor for screening and fractionation in the search for new bioactive natural compounds [32]. Lethality of substances to brine shrimp nauplii has been linked to the probable ability of such compounds to kill cancer cells in cell cultures [32].…”

“…The volume was made up to 5 mL with seawater (38 g/L, pH 7.4) and the vials were incubated at 25-27⁰C for 24 h under illumination. Other vials containing seawater and the reference cytotoxic drug (etoposide) served as the negative and positive controls respectively [32].…”

Euphorbia graminea Jacq. (Euphorbiaceae): In-Vitro Cytotoxic, Phytotoxic and Anti-Proliferative Assessment of the Extract and Fractions of the Leaves

“…The SRB (colorimetric sulforhodamine B assay) method designed by Khatun et al, 18 and described by Skehan et al, 19 was used to assess cytotoxicity against mouse Hela cell lines provided by the cell bank of the ICCBS. The rapidly growing exponential cells were harvested and suspended in growth media in a 96-well plate (100 mL, RPMI1640).…”