Comparative cytological studies of the effect of some aliphatic alcohols and the fatty alcohols from Euphorbia granulata and Pulicaria crispa on mitosis of Allium cepa.

Shehab, Abeer A.

doi:10.1508/cytologia.45.507

Cited by 2 publications

(1 citation statement)

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“…Despite extensive studies of the cross‐tolerance conferred by HSPs in yeast and animals, very little is known about this system in plants. Ethanol treatment was shown to alter the membrane permeability of root cells in Beta vulgaris ( Grunwald 1968), and was reported to inhibit mitotic division and induce abnormal chromosome numbers in Allium cepa root tissue ( Shehab 1980; Jones 1989). However, the effects of ethanol on HS gene expression and the synthesis of HSPs are unknown in plants.…”

Section: Introductionmentioning

confidence: 99%

Ethanol treatment triggers a heat shock‐like response but no thermotolerance in soybean (Glycine max cv. Kaohsiung No.8) seedlings

Kuo

Tsai

Young

et al. 2000

Plant Cell & Environment

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Non‐lethal heat‐shock (HS) treatment has previously been shown to induce thermotolerance in soybean (Glycine max cv. Kaohsiung No.8) seedlings. This acquired thermotolerance correlates with the de novo synthesis of heat‐shock proteins (HSPs). Interestingly, we found that ethanol treatments also elicited HS‐like responses in aetiolated soybean seedlings at their normal growth temperature of 28 °C. Northern blot analyses revealed that the expression of HS genes hsp17.5, hsp70 and hsc 70 was induced by ethanol. Radioactive amino acids were preferentially incorporated into high molecular weight (HMW) HSPs rather than class I low molecular weight (LMW) HSPs during non‐lethal ethanol treatments. Immunoblot analysis confirmed that no accumulation of class I LMW HSPs occurred after non‐lethal ethanol treatment. Pre‐treatment with a non‐lethal dose of ethanol did not provide thermotolerance, as the aetiolated soybean seedlings could not survive a subsequent heat shock of 45 °C for 2 h. In contrast, non‐lethal HS pre‐treatment, 40 °C for 2 h, conferred tolerance on aetiolated soybean seedlings to otherwise lethal treatments of 7·5% ethanol for 8 h or 10% ethanol for 4 h. These results suggest that plant class I LMW HSPs may play important roles in providing both thermotolerance and ethanol tolerance.

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Section: Introductionmentioning

confidence: 99%