Comparative cytokine gene expression: regulation and release by human mast cells

Möller,; Henz,; Grützkau,; Lippert, Rachel N.; Aragane,; Schwarz,; Krüger-Krasagakes,

doi:10.1046/j.1365-2567.1998.00425.x

Cited by 114 publications

(75 citation statements)

References 38 publications

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“…IL-2, IL-4, IL-7, IL-9, IL-12p35, IL-12p40, IL-14, IFN-␤, IFN-␥, lymphotactin, lymphotoxin-␤, TNF-␤, TGF-␤2, and TGF-␤3, mRNAs were not detectable in this assay. In addition, we detected the up-regulation of several cytokine transcripts by both Fc␥RI and Fc⑀RI aggregation that, to our knowledge, have not been previously described in human mast cells, including phorbol ester-treated human mast cell line 1 (22)(23)(24)(25)(26)(27); i.e., mRNAs for IP-10, LIF, M-CSF, OSM, and G-CSF. Upregulation of message for all chemokines and cytokines was determined after aggregation of receptors under conditions that lead to maximal histamine release and TNF-␣ generation (Fig.…”

Section: Discussionmentioning

confidence: 45%

“…In vitro studies with human mast cells and mast cell lines have demonstrated that these cells produce cytokines and chemokines (22)(23)(24)(25)(26)(27). Therefore, we next compared 38 cytokine and chemokine mRNA profiles using an RNase protection assay in IFN-␥-treated mast cells activated by either Fc␥RI or Fc⑀RI aggregation (Fig.…”

Section: Chemokine and Cytokine Mrna Profiles In Mast Cells After Eitmentioning

confidence: 99%

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A Comparison of Mediators Released or Generated by IFN-γ-Treated Human Mast Cells Following Aggregation of FcγRI or FcεRI

Okayama

Hagaman

Metcalfe

2001

The Journal of Immunology

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The high affinity receptor for IgG (FcγRI, CD64) is expressed on human mast cells, where it is up-regulated by IFN-γ and, thus, may allow mast cells to be recruited through IgG-dependent mechanisms in IFN-γ-rich tissue inflammation. However, the mediators produced by human mast cells after aggregation of FcγRI are incompletely described, and it is unknown whether these mediators are distinct from those produced after activation of human mast cells via FcεRI. Thus, we investigated the release of histamine and arachidonic acid metabolites and examined the chemokine and cytokine mRNA profiles of IFN-γ-treated cultured human mast cells after FcγRI or FcεRI aggregation. Aggregation of FcγRI resulted in histamine release and PGD2 and LTC4 generation. These responses were qualitatively indistinguishable from responses stimulated via FcεRI. Aggregation of FcεRI or FcγRI led to an induction or accumulation of 22 cytokine and chemokine mRNAs. Among them, seven cytokines (TNF-α, IL-1β, IL-5, IL-6, IL-13, IL-1R antagonist, and GM-CSF) were significantly up-regulated via aggregation of FcγRI compared with FcεRI. TNF-α mRNA data were confirmed by quantitative RT-PCR and ELISA. Furthermore, we confirmed histamine and TNF-α data using IFN-γ-treated purified human lung mast cells. Thus, aggregation of FcγRI on mast cells led to up-regulation and/or release of three important classes of mediators: biogenic amines, lipid mediators, and cytokines. Some cytokines, such as TNF-α, were released and generated to a greater degree after FcγRI aggregation, suggesting that selected biologic responses of mast cells may be preferentially generated through FcγRI in an IFN-γ-rich environment.

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Section: Discussionmentioning

confidence: 45%

Section: Chemokine and Cytokine Mrna Profiles In Mast Cells After Eitmentioning

confidence: 99%

A Comparison of Mediators Released or Generated by IFN-γ-Treated Human Mast Cells Following Aggregation of FcγRI or FcεRI

Okayama

Hagaman

Metcalfe

2001

The Journal of Immunology

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“…MC release a variety of factors known to enhance angiogenesis, namely heparin and histamine, 15,16 a variety of cytokines, such as transforming growth factor-beta, tumor necrosis factor-␣, 17 interleukin-8, 18 basic fibroblast growth factor or fibroblast growth factor-2 (FGF-2) 19 and vascular endothelial growth factor (VEGF) 20 implicated in normal, as well as tumorassociated angiogenesis. Blair et al 21 have shown that tryptase released by MC plays an important role in neovascularization.…”

Section: Discussionmentioning

confidence: 99%

Correlation of bone marrow angiogenesis and mast cells with tryptase activity in myelodysplastic syndromes

et al. 2002

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Bone marrow samples from 30 patients with myelodysplastic syndromes (MDS) grouped according to the International Prognostic Scoring System for MDS were investigated for counts of microvessels, total metachromatic mast cells (MC) and MC expressing tryptase, an angiogenesis-inducing molecule. Counts were higher in patients with a poor prognosis. The observation of a high correlation between microvessel counts and both total metachromatic and tryptase-reactive MC in all samples suggests that angiogenesis in MDS increases with their progression and that MC may intervene in the angiogenic response in MDS through tryptase contained in their secretory granules.

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“…Little is known about the cytokine profile of mucosa-derived human mast cells, because the cells can be obtained only in limited quantities and they are difficult to purify. The few data on cytokine production by human mast cells are mainly derived from immunohistochemical studies (5-7), or studies using immature bone marrow or cord blood-derived mast cells (8 -10), or the immature leukemic mast cell line HMC-1 (11)(12)(13)(14). Human lung mast cells were found to express mRNA for IL-4, IL-5, and GM-CSF and to release the products by IgE-dependent activation (15)(16)(17).…”

mentioning

confidence: 99%

Human Intestinal Mast Cells Are Capable of Producing Different Cytokine Profiles: Role of IgE Receptor Cross-Linking and IL-4

Lorentz

Schwengberg

Sellge

et al. 2000

The Journal of Immunology

157

116

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Mast cells are recognized as a new type of immunoregulatory cells capable of producing different cytokines. So far, little is known about the cytokine profile of mature human mast cells isolated from intestinal tissue and cultured in the presence of stem cell factor (SCF). We observed that these cells express the proinflammatory cytokines TNF-α, IL-1β, IL-6, IL-8, IL-16, and IL-18 without further stimulation. Both IgE-dependent and IgE-independent agonists (e.g., Gram-negative bacteria) enhanced expression of TNF-α. Another set of cytokines consisting of IL-3, IL-5, IL-9, and IL-13 was expressed following activation by IgE receptor cross-linking. If mast cells were cultured in the presence of IL-4 and SCF, the production and release of IL-3, IL-5, and IL-13 was increased up to 4-fold compared with mast cells cultured with SCF alone. By contrast, IL-6 expression was completely blocked in response to culture with IL-4. In summary, our data show that mature human mast cells produce proinflammatory cytokines that may be up-regulated following triggering with IgE-independent agonists such as bacteria, whereas activation by IgE receptor cross-linking results in the expression of Th2-type cytokines. IL-4 enhances the expression of Th2-type cytokines but does not affect or even down-regulates proinflammatory cytokines.

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Comparative cytokine gene expression: regulation and release by human mast cells

Cited by 114 publications

References 38 publications

A Comparison of Mediators Released or Generated by IFN-γ-Treated Human Mast Cells Following Aggregation of FcγRI or FcεRI

A Comparison of Mediators Released or Generated by IFN-γ-Treated Human Mast Cells Following Aggregation of FcγRI or FcεRI

Correlation of bone marrow angiogenesis and mast cells with tryptase activity in myelodysplastic syndromes

Human Intestinal Mast Cells Are Capable of Producing Different Cytokine Profiles: Role of IgE Receptor Cross-Linking and IL-4

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