Comparative cytogenetics of eight species of Cycloramphus (Anura, Cycloramphidae)

Noleto, Rafael Bueno; Amaro, Renata Cecília; Verdade, Vanessa Kruth; Campos, João Reinaldo Cruz; Gallego, Luiz Fernando Kraft; Lima, Ana Paula Weinfurter; Cestari, Marta Margarete; Kasahara, Sanae; Yonenaga‐Yassuda, Yatiyo; Rodrigues, Miguel Tréfaut; Toledo, Luı́s Felipe

doi:10.1016/j.jcz.2011.04.001

Cited by 8 publications

(11 citation statements)

References 39 publications

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“…lutzorum , and C . rhyakonastes [ 7 , 54 ]. Given this evidence, the most parsimonious hypothesis to account for these morphological changes in the homologs of pair 1 would involve centromeric reposition events that modified the plesiomorphic (metacentric) condition, which would have occurred in the lineage that gave rise to C .…”

Section: Discussionmentioning

confidence: 99%

“…asper Werner, 1899 [ 5 – 8 ]. Pericentromeric inversions or translocations are the primary sources of karyotype variation in this group [ 5 , 7 ]. The inclusion of new chromosomal markers with a phylogenetic approach to the analysis of the cytogenetic characteristics of the genus Cycloramphus [ 9 ] should contribute to the understanding of the mechanisms of chromosomal evolution that have molded the karyotypes of this genus.…”

Section: Introductionmentioning

confidence: 99%

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Cytogenetic characterization and mapping of the repetitive DNAs in Cycloramphus bolitoglossus (Werner, 1897): More clues for the chromosome evolution in the genus Cycloramphus (Anura, Cycloramphidae)

et al. 2021

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Cycloramphus bolitoglossus (Werner, 1897) is a rare species with a low population density in the Serra do Mar region of Paraná and Santa Catarina, in southern Brazil. Currently, it has been assigned to the Near Threatened (NT) category in the Brazilian List of Endangered Animal Species. Here, we described the karyotype of this species for the first time and investigated the patterns of some repetitive DNA classes in the chromosomes using molecular cytogenetic approaches. We isolated, sequenced and mapped the 5S rDNA and the satellite DNA PcP190 of C. bolitoglossus, as well as mapped the telomeric sequences and seven microsatellites motifies [(GA)15, (CA)15, (GACA)4, (GATA)8, (CAG)10, (CGC)10, and (GAA)]10. Cycloramphus bolitoglossus has 2n = 26 chromosomes and a fundamental number (FN) equal to 52, with a highly conserved karyotype compared to other genus members. Comparative cytogenetic under the phylogenetic context of genus allowed evolutionary interpretations of the morphological changes in the homologs of pairs 1, 3, and 6 along with the evolutionary history of Cycloramphus. Two subtypes of 5S rDNA type II were isolated in C. bolitoglossus genome, and several comparative analysis suggests mixed effects of concerted and birth-and-death evolution acting in this repetitive DNA. The 5S rDNA II subtype “a” and “b” was mapped on chromosome 1. However, their different position along chromosome 1 provide an excellent chromosome marker for future studies. PcP190 satellite DNA, already reported for species of the families Hylidae, Hylodidae, Leptodactylidae, and Odontophrynidae, is scattered throughout the C. bolitoglossus genome, and even non-heterochromatic regions showed hybridization signals using the PcP190 probe. Molecular analysis suggests that PcP190 satellite DNA exhibit a high-level of homogenization of this sequence in the genome of C. bolitoglossus. The PcP190 satDNA from C. bolitoglossus represents a novel sequence group, compared to other anurans, based on its hypervariable region. Overall, the present data on repetitive DNA sequences showed pseudogenization evidence and corroborated the hypothesis of the emergence of satDNA from rDNA 5S clusters. These two arguments that reinforced the importance of the birth-and-death evolutionary model to explain 5S rDNA patterns found in anuran genomes.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytogenetic characterization and mapping of the repetitive DNAs in Cycloramphus bolitoglossus (Werner, 1897): More clues for the chromosome evolution in the genus Cycloramphus (Anura, Cycloramphidae)

et al. 2021

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“…Cytological techniques has been successfully applied to anuran groups as a valuable tool to investigate chromosome evolution (Busin et al 2001; Campos et al 2009), infer species relationships (Noleto et al 2011; Cardozo et al 2011), corroborate suggestion of new species (Giaretta and Aguiar Jr. 1998; Toledo et al 2010), and differentiate cryptic species (Siqueira et al 2009).…”

mentioning

confidence: 99%

Cytogenetic analysis of two species in the Phyllomedusa hypochondrialis group (Anura, Hylidae)

et al. 2012

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The genus Phyllomedusa has been the target of regular taxonomic investigations. The species Phyllomedusa nordestina was recently separated from P. hypochondrialis. Morphological variations in the P. rohdei interpopulation have already been reported, suggesting the existence of more than one taxon under that name. In the present study, we have cytogenetically characterized two populations of P. nordestina and one of P. rohdei. Both species displayed 2n = 26 chromosomes with 12 metacentric, 12 submetacentric and 2 subtelocentric chromosomes. The C-banding analyses revealed discrete differences in the quantity of centromeric heterochromatin between the two species. The nucleolus organizer region (NOR) was detected in pair 9 of both species, but is located in the pericentromeric region of the short arm in P. nordestina and in the long arm subtelomeric region of P. rohdei. Chromosomal data from this study indicate karyotypic homeology between the two groups of P. hypochondrialis species and suggest the existence of more than one taxon under the P. rohdei name.

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“…An association between constitutive heterochromatin and rDNA cistrons is frequently reported in anurans (e.g. Amaro-Ghilardi et al 2008;Campos et al 2009;Noleto et al 2011). In three of the four analysed Dendropsophus species, pair 13 was identified as the NOR-bearing pair and the NOR was always positioned in the telomeric region of the long arm.…”

Section: Nor: Correlation With Heterochromatin and Diversitymentioning

confidence: 64%

“…However, while D. elegans individuals showed that chromosome pair 13 was the NOR carrier, individuals from Ubatuba presented multiple NOR locations on pairs 10 and 14 (Gruber et al 2005). This difference could represent an interpopulation NOR polymorphism which seems to be common in other anurans (Medeiros et al 2003;Noleto et al 2011). Multiple NOR-bearing chromosomal pairs are considered as a derived state in anurans (King et al 1990).…”

Section: Nor: Correlation With Heterochromatin and Diversitymentioning

confidence: 99%

Comparative cytogenetic analysis of four species of Dendropsophus (Hylinae) from the Brazilian Atlantic forest

Oliveira

Noleto

Oliveira

et al. 2016

J Genet

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We conducted a cytogenetic study of four hyline frog species (Dendropsophus elegans, D. microps, D. minutus and D. werneri) from southern Brazil. All species had 2n = 30 chromosomes, with interspecific and intraspecific variation in the numbers of metacentric, submetacentric, subtelocentric and telocentric chromosomes. C-banding and fluorochrome staining revealed conservative GC-rich heterochromatin localized in the pericentromeric regions of all species. The location of the nucleolus organizer regions, as confirmed by fluorescent in situ hybridization, differed between species. Telomeric probes detected sites that were restricted to the terminal regions of all chromosomes and no interstitial or centromeric signals were observed. Our study corroborates the generic synapomorphy of 2n = 30 chromosomes for Dendropsophus and adds data that may become useful for future taxonomic revisions and a broader understanding of chromosomal evolution among hylids.

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Comparative cytogenetics of eight species of Cycloramphus (Anura, Cycloramphidae)

Cited by 8 publications

References 39 publications

Cytogenetic characterization and mapping of the repetitive DNAs in Cycloramphus bolitoglossus (Werner, 1897): More clues for the chromosome evolution in the genus Cycloramphus (Anura, Cycloramphidae)

Cytogenetic characterization and mapping of the repetitive DNAs in Cycloramphus bolitoglossus (Werner, 1897): More clues for the chromosome evolution in the genus Cycloramphus (Anura, Cycloramphidae)

Cytogenetic analysis of two species in the Phyllomedusa hypochondrialis group (Anura, Hylidae)

Comparative cytogenetic analysis of four species of Dendropsophus (Hylinae) from the Brazilian Atlantic forest

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