Comparative Chemical and Biological Activity of Selective Herbal Extracts

Humulescu, Ioana

doi:10.31925/farmacia.2021.5.5

Cited by 5 publications

(9 citation statements)

References 22 publications

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“…The contents of luteolin and apigenin in the ME of air-dried T. montanum from Romania were 1.1775 and 0.6531 mg/g dry extract, respectively, while the content of caffeic acid was 1.6185 mg/g dry extract. No content of rosmarinic acid was detected in the studied extracts [33].…”

Section: The Content Of Phenol Compounds In Extracts Of T Montanummentioning

confidence: 78%

“…The total phenol content of ME of the plant from the northeastern region of Romania was 164.42 ± 0.03 mg GAE/g dry extract, while the total flavonoid content was 51.42 ± 0.15 mg GAE/g dry extract [33].…”

Section: The Content Of Phenol Compounds In Extracts Of T Montanummentioning

confidence: 90%

“…The antioxidant activity of ME of T. montanum from northeastern Romania tested by the DPPH method was IC 50 = 50.91 ± 0.21 µg/mL [33].…”

Section: Antioxidant Potential Of Essential Oil And/or Extracts Isola...mentioning

confidence: 97%

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Phytochemical Profile, Antioxidant Activity and Cholinesterase Inhibition Potential of Essential Oil and Extracts of Teucrium montanum from Bosnia and Herzegovina

et al. 2023

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Bioactive compounds from plants play an important role in slowing many neurodegenerative diseases, such as Alzheimer’s disease, by inhibiting cholinesterase enzymes. Studies have shown that oxidative stress is associated with the development of Alzheimer’s disease. In traditional medicine of Bosnia and Herzegovina, Teucrium montanum is used to treat numerous diseases. The chemical composition and biological activity of the essential oil (EO) and aqueous (AE) and methanol extract (ME) of this plant were studied. The chemical composition of EO was studied using GC-MS, while the composition of the extracts was studied using HPLC-DAD. Antioxidant activity was tested using the DPPH and FRAP methods. The protection of lipids and proteins from oxidation was tested using the ammonium thiocyanate and BSA oxidation methods. The ability to inhibit cholinesterases was tested by the Ellman method. The main identified EO compounds were α-cadinol, ß-selinene, δ-cadinene, epi-α-cadinol, germacrene D-4-ol, and α-pinene. The main phenolic compounds of the extracts were p-coumaric acid, ellagic acid and caffeic acid. The tested extracts showed good antioxidant radical scavenging and reducing potential and a very good ability to protect lipids and proteins from oxidation. The EO showed moderate AChE and BChE inhibition potential, while the extracts showed weak or no ability.

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Section: The Content Of Phenol Compounds In Extracts Of T Montanummentioning

confidence: 78%

Section: The Content Of Phenol Compounds In Extracts Of T Montanummentioning

confidence: 90%

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Phytochemical Profile, Antioxidant Activity and Cholinesterase Inhibition Potential of Essential Oil and Extracts of Teucrium montanum from Bosnia and Herzegovina

et al. 2023

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“…A review of the literature indicated that aqueous mixtures organic solvents are better solvents for extraction of polyphenolic compounds from plant material [18,29,30,32]. Humulescu et al [22] point out that in addition to solvents, other factors such as dryness, dimension of plant material, temperature, pressure for processing and human factors affect yield extraction. In plants, polyphenols perform a number of functions that have a great impact on the ecophysiology of plants: they act as antioxidants, antimicrobial agents, visual attractants of some insects important for flower pollination [19].…”

Section: Introductionmentioning

confidence: 99%

Influence of Solvent Type on the Phenolic Content and Antimicrobial and Antioxidant Properties of Echium Vulgare L. Extracts

BOŠKOVIĆ¹

2022

FARMACIA

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The aim of this study was to investigate influence of solvent type on the phenolic content and antimicrobial and antioxidant properties of Echium vulgare L. extracts. The following solvents were used in the study: ethanol, acetone, chloroform, petroleum and ethyl acetate. The content of phenols, flavonoids and tannins was determined by spectrophotometric methods, while the identification and quantification of polyphenolic compounds was performed by chromatographic method. Determination of antimicrobial activity was done by microdilution method, while several methods were used in the evaluation of antioxidant activity of plant extracts. The ethanolic extract had the highest content of phenols and flavonoids, while chloroform extract had the highest content of tannins. The ethanolic extract exhibited the best antioxidant potential compared to other extracts, and ethanolic and chloroform extracts manifested the best antimicrobial activity. RezumatScopul acestui studiu a fost de a investiga influența tipului de solvent asupra conținutului fenolic, a proprietăților antimicrobiene și antioxidante ale extractelor obținute de la specia Echium vulgare L. Au fost utilizați ca solvenți: etanol, acetonă, cloroform, petrol și acetat de etil, iar conținutul în fenoli, flavonoizi și taninuri a fost determinat prin metode spectrofotometrice. Identificarea și cuantificarea compușilor polifenolici s-a realizat prin metoda cromatografică. Determinarea activității antimicrobiene s-a realizat prin metoda microdiluției, în timp ce pentru evaluarea activității antioxidante a extractelor au fost utilizate mai multe metode. Extractul etanolic a avut cel mai mare conținut de fenoli și flavonoide, în timp ce extractul cloroformic a avut cel mai mare conținut de taninuri. Extractul etanolic a prezentat cel mai pronunțat potențial antioxidant, în timp ce extractele etanolice și cloroformice au manifestat cea mai bună activitate antimicrobiană.

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“…Starting from the method described by Giusti and Wrolstad in 2001, the total anthocyanin content was evaluated by using two dilutions of the same extract and changing the pH between 1.0 and 4.5. It is known that the chemical form of anthocyanin radicals (oxonium) has different absorption spectra when the acidic environment changes [19].…”

Section: Total Anthocyanin Quantificationmentioning

confidence: 99%

Assessing the Antioxidant Properties, In Vitro Cytotoxicity and Antitumoral Effects of Polyphenol-Rich Perilla leaves Extracts

Adam,

Cojocaru,

Verestiuc

et al. 2023

Antioxidants

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(1) Background: This study aimed to outline the antioxidant, antitumoral, and cytotoxic proprieties of various types of Perilla frutescens extracts obtained from the leaves of the species. (2) Methods: We determined total polyphenols, flavonoids and anthocyanins contents, as well as the in vitro antioxidant, antitumoral, and cytotoxic actions in three types of ethanolic extracts (E1, E2, E3) and in three types of acetone: ethanol extracts (A1, A2, A3) of Perilla frutescens according to standardized procedures. (3) Results: We found that Perilla frutescens ethanolic extracts had the highest total phenol and anthocyanins concentrations. The flavonoids concentration was not statistically different between the extracts. The iron chelating capacity, hydroxyl radical scavenging capacity, superoxide anion radical scavenging capacity, and lipoxygenase inhibition capacity showed a significant increase with higher concentrations of Perilla frutescens extracts, particularly the ethanolic extracts. Perillyl alcohol had greater cytotoxic capacity in the MG-63 cell line and E1 extract showed similar significant cytotoxic effects in the A431 cell line. (4) Conclusions: Both ethanolic and acetone–ethanol extracts from Perilla frutescens exhibited important antioxidant and antitumoral actions in vitro, which proportionally increased with concentration. The cytotoxic threshold determined in this study for various types of extracts could help determine the best dosage with the maximum antioxidant and antitumoral potential. Our results could serve as a basis for further studies that will investigate the cytotoxic effects of Perilla frutescens variants on various types of cancer cell lines.

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Comparative Chemical and Biological Activity of Selective Herbal Extracts

Cited by 5 publications

References 22 publications

Phytochemical Profile, Antioxidant Activity and Cholinesterase Inhibition Potential of Essential Oil and Extracts of Teucrium montanum from Bosnia and Herzegovina

Phytochemical Profile, Antioxidant Activity and Cholinesterase Inhibition Potential of Essential Oil and Extracts of Teucrium montanum from Bosnia and Herzegovina

Influence of Solvent Type on the Phenolic Content and Antimicrobial and Antioxidant Properties of Echium Vulgare L. Extracts

Assessing the Antioxidant Properties, In Vitro Cytotoxicity and Antitumoral Effects of Polyphenol-Rich Perilla leaves Extracts

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